本文選題：環(huán)磷酰胺 + 骨髓抑制　； 參考：《河南中醫(yī)藥大學(xué)》2016年碩士論文

【摘要】：目的通過觀察在針刺和艾灸不同作用下,環(huán)磷酰胺(Cyclophosphamide,CTX)化療小鼠血清中造血細(xì)胞因子FIt3配體(flt3 ligand,FL)與巨噬細(xì)胞炎性蛋白-1α(macrophage inflammatory protein-1α,MIP-1α)的變化,闡釋針灸改善CTX化療小鼠骨髓造血微環(huán)境,保護(hù)造血干細(xì)胞,提升外周血白細(xì)胞的分子生物學(xué)機(jī)制,為針灸改善化療所致小鼠造血微環(huán)境損傷方面提供實(shí)驗(yàn)研究依據(jù)。方法選取SPF級(jí)雄性昆明種小鼠80只(7周齡、體重22±2g),根據(jù)治療天數(shù)不同依據(jù)體重分層隨機(jī)分兩大組,即3d組和5d組,每組40只。再按其體重分層隨機(jī)分成8小組:空白組(A3組、A5組)、模型組(B3組、B5組)、針刺組(C3組、C5組)、艾灸組(D3組、D5組),每亞組各10只。空白組根據(jù)小鼠體重按照濃度0.02ml/g給予生理鹽水腹腔注射,連續(xù)3d。其余4組腹腔注射CTX,根據(jù)小鼠體重0.02ml/g,注射CTX濃度為5mg/ml,即100mg/kg/d,連續(xù)3d,停藥后4小時(shí)骨髓抑制模型即成。治療時(shí)空白組、模型組陪同固定,不做治療。針刺組、艾灸組選取“大椎”、“膈俞”、“腎俞”、“足三里”四穴給予針刺和艾灸治療,連續(xù)治療3d和5d。實(shí)驗(yàn)期間每日定時(shí)專人割破鼠尾靜脈取血,鏡檢外周血白細(xì)胞計(jì)數(shù)。各組小鼠分別在治療3d、5d后的第2d摘眼球取血,離心,取上清液,取材后放入-80℃低溫冰箱保存、備檢。送檢材料采用ELISA法檢測(cè)小鼠血清中FL和MIP-1α的含量。運(yùn)用SPSS20.0 for Windows軟件進(jìn)行數(shù)據(jù)統(tǒng)計(jì)分析。結(jié)果1造模后,與空白組對(duì)照,剩余3組外周血白細(xì)胞數(shù)量大幅度降低,有統(tǒng)計(jì)學(xué)差異(P0.05),且3組之間降低幅度相差不多,說明骨髓抑制模型成功。治療3d后,與模型組比較,針刺組和艾灸組白細(xì)胞計(jì)數(shù)有升高趨勢(shì),但無統(tǒng)計(jì)學(xué)差異(P0.05)。治療5d后,針刺組和艾灸組白細(xì)胞計(jì)數(shù)已達(dá)到并超過空白組,有統(tǒng)計(jì)學(xué)差異(P0.05),與模型組比較,有統(tǒng)計(jì)學(xué)差異(P0.05)。2經(jīng)過3d的治療,與模型組比較,針刺組和艾灸組中血清中FL含量表達(dá)均降低,有統(tǒng)計(jì)學(xué)差異(P0.05);與針刺組比較,艾灸組中的含量表達(dá)偏低,但無統(tǒng)計(jì)學(xué)差異(P0.05)。治療5天后,針刺組與艾灸組中含量表達(dá)仍低于模型組,有統(tǒng)計(jì)學(xué)差異(P0.05);與針刺組比較,艾灸組含量表達(dá)依然偏低,仍無統(tǒng)計(jì)學(xué)差異(P0.05)。3經(jīng)過3d治療,與模型組相比,針刺組和艾灸組血清中MIP-1α含量表達(dá)均高于模型組,有統(tǒng)計(jì)學(xué)差異(P0.05);與針刺組對(duì)比,艾灸組表達(dá)含量偏高,但無統(tǒng)計(jì)學(xué)差異(P0.05)。治療5d后,與模型組比較,針刺組和艾灸組中的含量表達(dá)均高于模型組,有統(tǒng)計(jì)學(xué)差異(P0.05);與針刺組比較,艾灸組中含量表達(dá)仍偏高,但無統(tǒng)計(jì)學(xué)差異(P0.05)。結(jié)論1針灸改善化療后骨髓抑制,升高白細(xì)胞的療效是確切的,再次佐證導(dǎo)師組前期研究成果及規(guī)律,針刺和艾灸可使CTX化療小鼠白細(xì)胞較模型組提前1d回升并提前1d超過基礎(chǔ)水平。2化療后小鼠血清中FL的含量表達(dá)明顯升高,針刺、艾灸可以下調(diào)化療小鼠血清中FL的含量表達(dá),并且將FL水平維持在適當(dāng)濃度,以利于早期造血細(xì)胞的存活、增殖、分化,修復(fù)損傷的造血微環(huán)境,改善化療后骨髓抑制。3化療后小鼠血清中MIP-1α的表達(dá)含量降低。通過針刺、艾灸的作用,化療小鼠血清中MIP-1α的含量表達(dá)升高,既可以選擇性的保護(hù)造血干細(xì)胞,又能協(xié)同集落刺激因子促集落的形成,以促進(jìn)骨髓造血干/祖細(xì)胞的增殖和成熟,改善骨髓造血微環(huán)境,提升外周血白細(xì)胞計(jì)數(shù)。4針灸可促進(jìn)化療后骨髓抑制盡快得到改善,損傷的造血微環(huán)境能夠得到恢復(fù),且治療作用可隨治療天數(shù)的增加而增加,具有治療意義,艾灸作用優(yōu)于針刺。
[Abstract]:Objective To observe the changes of hematopoietic cell factor FIt3 ligand (Flt3 ligand, FL) and macrophage inflammatory protein -1 alpha (macrophage inflammatory protein-1 a, MIP-1 alpha) in the serum of mice treated with cyclophosphamide (Cyclophosphamide, CTX) under different effects of acupuncture and moxibustion, and to explain the improvement of hematopoietic microenvironment and protection of bone marrow in mice with chemotherapy by Acupuncture and moxibustion. Hematopoietic stem cells, promoting the molecular biological mechanism of peripheral blood leucocyte to provide experimental basis for the improvement of hematopoietic microenvironment injury induced by chemotherapy in mice. Methods 80 male Kunming mice (7 weeks of age, 22 + 2G) were selected and divided into two groups according to the number of days of treatment, that is, group 3D and 5D, each group. Group 40 were divided randomly into 8 groups according to their weight stratification: blank group (group A3, group A5), model group (group B3, B5), acupuncture group (group C3, C5), moxibustion group (group D3, D5), 10 in each subgroup. The blank group was given abdominal injection of normal saline on the weight of mice according to the concentration 0.02ml/g, and the rest of the other 4 groups were injected CTX, according to mice weight 0.02ml/g. The concentration of CTX was 5mg/ml, 100mg/kg/d, continuous 3D, and the bone marrow suppression model was formed 4 hours after the drug stopped. The time and space white group was treated. The model group was accompanied by fixed, no treatment. The acupuncture group, the moxibustion group selected the four points of "Da Zhui", "Shenshu", "Shenshu", "Zusanli", given acupuncture and moxibustion for the continuous treatment of 3D and 5d. experiments. During the treatment of 3D and 5D, the mice were removed from the rat's tail vein to take blood and take the blood from the 3D and 5D after the treatment. After the centrifugation, the supernatant was centrifuged, and then stored at -80 centigrade cryogenic refrigerator. The content of FL and MIP-1 alpha in the serum of mice was detected by ELISA. SPSS20.0 for Windows software was used. Data statistical analysis. Results after 1 models, compared with the blank group, the number of white blood cells in the remaining 3 groups decreased significantly (P0.05), and the difference between the 3 groups was not much, indicating that the bone marrow suppression model was successful. After the treatment of 3D, the white blood cell count of the acupuncture group and the moxibustion group was higher than that in the model group, but there was no statistical difference. Difference (P0.05). After the treatment of 5D, the white blood cell count of the acupuncture group and the moxibustion group had reached and exceeded the blank group. There was a statistical difference (P0.05). Compared with the model group, there was a statistical difference (P0.05).2 after 3D treatment. Compared with the model group, the expression of FL content in the serum of acupuncture group and moxibustion group decreased, with statistical difference (P0.05), compared with the acupuncture group, and compared with the acupuncture group, and compared with the acupuncture group. Compared with the moxibustion group, the expression of the content was low, but there was no statistical difference (P0.05). The contents of the acupuncture group and the moxibustion group were still lower than the model group (P0.05) after 5 days of treatment. Compared with the acupuncture group, the content of the moxibustion group was still low, and there was still no difference (P0.05).3 after 3D treatment. Compared with the model group, the acupuncture group and moxibustion were compared with the model group. The expression of MIP-1 alpha in the serum of the group was higher than that in the model group (P0.05). Compared with the acupuncture group, the expression level of the moxibustion group was high, but there was no statistical difference (P0.05). After the treatment of 5D, the content expression in the acupuncture group and moxibustion group was higher than that in the model group, with a statistical difference (P0.05); compared with the acupuncture group, the moxibustion group was compared with the moxibustion group. The expression of the content was still high, but there was no statistical difference (P0.05). Conclusion 1 the effect of acupuncture and moxibustion on the improvement of bone marrow suppression after chemotherapy and the increase of white blood cells is exact. Again, the results and rules of the early study of the tutor group are confirmed again. Acupuncture and moxibustion can make the leukocytes in the CTX chemotherapy mice advance 1D ahead of time in the model group and advance to the blood of the mice after the basic level of.2 chemotherapy. The content expression of FL in the Qing Dynasty increased obviously. Acupuncture and moxibustion could reduce the expression of FL in the serum of chemotherapy mice, and maintain the level of FL at the appropriate concentration to help the survival, proliferation, differentiation and repair of the hematopoietic microenvironment of the early hematopoietic cells, and improve the expression of MIP-1 alpha in the serum of mice after chemotherapy after chemotherapy, and to improve the expression of MIP-1 alpha in the serum of mice after.3 chemotherapy. Under the effect of acupuncture and moxibustion, the expression of MIP-1 alpha in serum of chemotherapy mice increased, which could selectively protect hematopoietic stem cells and synergy the formation of colony stimulating factors to promote the proliferation and maturation of bone marrow hematopoietic stem / progenitor cells, improve the microenvironment of bone marrow blood making and increase the peripheral blood white blood cell count.4 acupuncture and moxibustion After the promotion of chemotherapy, bone marrow suppression is improved as soon as possible. The hematopoietic microenvironment can be recovered, and the therapeutic effect can be increased with the increase of the number of days of treatment. It is of therapeutic significance. Moxibustion is superior to acupuncture.
【學(xué)位授予單位】：河南中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R245

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 柯長(zhǎng)鴻;艾灸治療熱病驗(yàn)案2則[J];江西中醫(yī)藥;2001年05期

2 張麗穎,王晶,尹麗曼;體針配合艾灸治療癃閉16例[J];針灸臨床雜志;2001年06期

3 徐宓宓;艾灸治療失眠79例[J];實(shí)用中醫(yī)藥雜志;2001年10期

4 王玉紅;艾灸治療小兒腸系膜淋巴結(jié)炎85例[J];中國(guó)社區(qū)醫(yī)師;2003年09期

5 王玉紅;艾灸治療小兒腸系膜淋巴結(jié)炎85例[J];中國(guó)民間療法;2003年03期

6 袁志明,徐清波;艾灸治療瘰疬65例[J];中國(guó)針灸;2004年09期

7 費(fèi)景蘭;劉姝;馮寶榮;;艾灸治療術(shù)后呃逆28例[J];中國(guó)臨床醫(yī)生;2006年02期

8 劉春華;孫春紅;馬保賢;;艾灸治療術(shù)后腹脹[J];浙江中醫(yī)雜志;2006年03期

9 朱靜;李俊霞;;電針加艾灸治療難治性面癱60例[J];醫(yī)學(xué)理論與實(shí)踐;2006年05期

10 張燕超;楊威;;艾灸過敏1例[J];北京中醫(yī);2006年06期

相關(guān)會(huì)議論文 前10條

1 肖艷冰;;實(shí)測(cè)經(jīng)絡(luò)電針配合艾灸治療慢性潰瘍性結(jié)腸炎16例[A];第8屆全國(guó)針灸科研與臨床研討會(huì)暨安徽省針灸學(xué)會(huì)2009年年會(huì)論文集[C];2009年

2 周志杰;張福會(huì);黃麗娜;姚益龍;;蘄艾灸治療產(chǎn)后排尿異常54例療效觀察[A];中國(guó)針灸學(xué)會(huì)2005年學(xué)術(shù)年會(huì)論文匯編[C];2005年

3 王慶雷;路聚更;李中堂;樊翠紅;王紅全;陳紅旗;陳書秀;;艾艾灸治療艾滋病效果分析[A];中華中醫(yī)藥學(xué)會(huì)防治艾滋病分會(huì)第八次年會(huì)論文集[C];2011年

4 陳鐵雷;;艾灸對(duì)人類健康的影響[A];中國(guó)針灸學(xué)會(huì)臨床分會(huì)全國(guó)第十九屆針灸臨床學(xué)術(shù)研討會(huì)論文集[C];2011年

5 王偉;劉梅;王立公;;多功能艾灸治療床的研制意義及臨床應(yīng)用[A];中國(guó)康復(fù)醫(yī)學(xué)會(huì)第22屆療養(yǎng)康復(fù)學(xué)術(shù)會(huì)議論文匯編[C];2011年

6 周春姣;楊麗明;李莉;胡瓊;;腧穴熱敏化艾灸早期干預(yù)術(shù)后病人便秘的療效觀察[A];第七屆全國(guó)中西醫(yī)結(jié)合圍手術(shù)期醫(yī)學(xué)專題研討會(huì)全國(guó)中西醫(yī)結(jié)合圍手術(shù)期快速康復(fù)新進(jìn)展培訓(xùn)班廣東省中醫(yī)藥學(xué)會(huì)外科學(xué)會(huì)會(huì)議論文集[C];2012年

7 王靜;黃穗平;鄺宇香;;中藥配合艾灸治療腹瀉型腸易激綜合征30例[A];中華中醫(yī)藥學(xué)會(huì)第二十一屆全國(guó)脾胃病學(xué)術(shù)交流會(huì)暨2009年脾胃病診療新進(jìn)展學(xué)習(xí)班論文匯編[C];2009年

8 成玉;勒怨悛;;艾灸治療白癜風(fēng)22例療效觀察[A];2011全國(guó)中西醫(yī)結(jié)合皮膚性病學(xué)術(shù)會(huì)議論文匯編[C];2011年

9 王思成;曹烈虎;李卓東;張前進(jìn);扶小明;蘇佳燦;張春才;;艾灸治療膝骨性關(guān)節(jié)炎臨床療效觀察[A];2009第十七屆全國(guó)中西醫(yī)結(jié)合骨傷科學(xué)術(shù)研討會(huì)論文匯編[C];2009年

10 李曉泓;韓毳;張露芬;郭順根;;艾灸抗應(yīng)激作用的實(shí)驗(yàn)研究[A];第六次全國(guó)中西醫(yī)結(jié)合實(shí)驗(yàn)醫(yī)學(xué)學(xué)術(shù)研討會(huì)會(huì)議論文集[C];2002年

相關(guān)重要報(bào)紙文章 前10條

1 本報(bào)記者 張星海;艾灸流行：新時(shí)代的迷信[N];北京科技報(bào);2010年

2 靳朝輝　王麗霞;開創(chuàng)艾灸新療法 拓展行業(yè)新坐標(biāo)[N];中國(guó)社會(huì)科學(xué)報(bào);2013年

3 本報(bào)記者 周穎;小艾灸 防大病[N];中國(guó)中醫(yī)藥報(bào);2010年

4 宋南昌;艾灸治療帶狀皰疹[N];家庭醫(yī)生報(bào);2007年

5 安徽省中醫(yī)院 秦黎虹;艾灸治療糖尿病足[N];健康報(bào);2009年

6 早報(bào)記者 肖婷;家庭保健艾灸將展開首輪推廣[N];東方早報(bào);2011年

7 宋南昌;艾灸治療急性尿潴留[N];家庭醫(yī)生報(bào);2008年

8 楊慎峭 劉旭光 余曙光;艾灸治療關(guān)節(jié)炎機(jī)制與細(xì)胞因子有關(guān)[N];中國(guó)醫(yī)藥報(bào);2005年

9 宋南昌;艾灸治療支氣管哮喘[N];家庭醫(yī)生報(bào);2007年

10 宋南昌;艾灸治療蕁麻疹[N];家庭醫(yī)生報(bào);2008年

相關(guān)博士學(xué)位論文 前6條

1 張耀;基于膽堿能抗炎通路的艾灸治療實(shí)驗(yàn)性RA作用機(jī)制研究[D];成都中醫(yī)藥大學(xué);2013年

2 羅榮;交感神經(jīng)在艾灸治療實(shí)驗(yàn)性RA中的作用機(jī)理研究[D];成都中醫(yī)藥大學(xué);2003年

3 鄭欣;膝三針配合艾灸治療老年膝關(guān)節(jié)退行性骨關(guān)節(jié)炎臨床研究[D];廣州中醫(yī)藥大學(xué);2009年

4 楊馨;艾灸調(diào)控RA滑膜細(xì)胞功能的JAK-STAT信號(hào)通路負(fù)反饋機(jī)制研究[D];成都中醫(yī)藥大學(xué);2014年

5 馬文彬;艾灸治療RA抗炎效應(yīng)的HPAA節(jié)律機(jī)制研究[D];成都中醫(yī)藥大學(xué);2014年

6 劉獻(xiàn)祥;針灸對(duì)絕經(jīng)后骨質(zhì)疏松癥影響的實(shí)驗(yàn)和臨床研究[D];福建中醫(yī)學(xué)院;2002年

相關(guān)碩士學(xué)位論文 前10條

1 高宇晨;艾灸對(duì)調(diào)控IL-17的佐劑型RA大鼠足趾腫脹度與形態(tài)學(xué)的影響變化[D];南京中醫(yī)藥大學(xué);2015年

2 黎海軍;艾灸治療實(shí)驗(yàn)性RA關(guān)節(jié)骨破壞的破骨細(xì)胞機(jī)制研究[D];成都中醫(yī)藥大學(xué);2015年

3 耿煜;艾灸對(duì)潰瘍性結(jié)腸炎模型小鼠的血漿HPLC-MS代謝組學(xué)研究[D];成都中醫(yī)藥大學(xué);2015年

4 何蛟龍;基于光聲斷層掃描成像系統(tǒng)的艾灸實(shí)驗(yàn)研究[D];電子科技大學(xué);2016年

5 徐驍;艾灸治療類風(fēng)濕關(guān)節(jié)炎的系統(tǒng)性評(píng)價(jià)及其治療機(jī)制的蛋白質(zhì)組學(xué)實(shí)驗(yàn)研究[D];南京中醫(yī)藥大學(xué);2016年

6 何道有;艾灸頭部組穴對(duì)VD模型大鼠認(rèn)知功能及神經(jīng)—血管共生影響的研究[D];安徽中醫(yī)藥大學(xué);2016年

7 周俊梅;艾灸對(duì)不同腫瘤模型的抑瘤機(jī)制研究[D];上海交通大學(xué);2015年

8 張欣;益君康聯(lián)合艾灸治療腹瀉型腸易激綜合征的臨床觀察[D];山東中醫(yī)藥大學(xué);2016年

9 王佳麗;針灸對(duì)環(huán)磷酰胺化療小鼠血清中FL與MIP-1α含量影響的研究[D];河南中醫(yī)藥大學(xué);2016年

10 馮淑儀;艾灸治療術(shù)后疲勞綜合征的臨床研究[D];廣州中醫(yī)藥大學(xué);2012年

，

本文編號(hào)：2097919