本文選題：清腦益元湯 + 腦缺血損傷　； 參考：《廣西中醫(yī)藥大學(xué)》2016年碩士論文

【摘要】：目的:觀察清腦益元湯對大鼠腦缺血損傷缺血側(cè)皮層及海馬區(qū)VEGF、PDGF表達(dá)的影響,探討清腦益元湯對大鼠腦缺血損傷神經(jīng)元保護(hù)的作用機(jī)制。方法:將成年健康雄性SD大鼠192只,按隨機(jī)數(shù)字表分為兩大組,每組96只,繼續(xù)隨機(jī)分為4個亞組:空白組(A組6只)、假手術(shù)組(B組30只)、模型對照組(C組30只)、清腦益元組(D組30只)。假手術(shù)組、模型對照組及清腦益元組均按缺血損傷后1d、3d、7d、14d、28d五個處死時間點(diǎn)分為5個亞組(n=6),并分別作如下處理:空白正常飼養(yǎng),灌胃等體積蒸餾水;假手術(shù)組單純手術(shù)而未插線動物,術(shù)后正常飼養(yǎng),灌胃等體積蒸餾水;模型對照組灌胃等體積蒸餾水7d后,麻醉,行MACO術(shù),術(shù)后制成大鼠急性腦缺血損傷大鼠模型,待大鼠麻醉清醒后,繼續(xù)予生理鹽水灌胃至各處死時間點(diǎn)。清腦益元組灌胃等體積清腦益元7d后操作同模型對照組,待大鼠麻醉清醒后,繼續(xù)予中藥灌胃至大鼠各處死時間點(diǎn)。參考Longa的5分制法對大鼠進(jìn)行神經(jīng)功能缺損評分,并按各時間點(diǎn)進(jìn)行標(biāo)本采集。取材后,一組應(yīng)用實(shí)時定量逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(Real Time PCR)檢測大鼠缺血側(cè)皮層及海馬區(qū)VEGF的表達(dá),另一組應(yīng)用蛋白質(zhì)印跡法(Western Blot)檢測大鼠缺血側(cè)皮層及海馬區(qū)PDGF的表達(dá)。結(jié)果:1.RT-PCR檢測結(jié)果:與假手術(shù)組比較,模型組腦組織VEGF的mRNA表達(dá)在各時間點(diǎn)均有所升高,但統(tǒng)計(jì)學(xué)比較無顯著差異。與模型組比較,清腦益元組VEGF mRNA表達(dá)在各時間點(diǎn)均顯著增高。清腦益元組早期(第1天)可見VEGF表達(dá)增高,在早期(第3天)就明顯升高,并逐漸升高第14天達(dá)到高峰;在模型對照組VEGF表達(dá)成緩慢增長的趨勢,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。2.蛋白質(zhì)印跡法結(jié)果:與模型組比較,清腦益元組PDGF的表達(dá)在各時間點(diǎn)均顯著增高。清腦益元組早期(第1天)可見PDGF的明顯增高,在早期(第3天)呈稍放緩但仍很明顯的增高趨勢,并逐漸升高第14天達(dá)到高峰;在模型組PDGF表達(dá)成緩慢增長的趨勢,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:清腦益元湯對大鼠腦缺血損傷神經(jīng)元的保護(hù)作用可能與VEGF、PDGF表達(dá)的增加有關(guān)。
[Abstract]:Objective: to observe the effect of Qingnao Yiyuan decoction (QNYD) on the expression of VEGF PDGF in ischemic cortex and hippocampus of rats with cerebral ischemia injury, and to explore the protective mechanism of Qingnao Yiyuan decoction (QIYD) on neurons injured by cerebral ischemia in rats. Methods: 192 adult male SD rats were randomly divided into two groups, 96 rats in each group. They were randomly divided into four subgroups: group A (n = 6), group B (n = 30), group C (n = 30) and group D (n = 30). Sham-operation group, model control group and Qingnao Yiyuan group were divided into 5 subgroups according to five death time points: normal blank feeding and gastric distilled water. In the sham operation group, the rats were fed normally and fed with distilled water of the same volume after operation, and the rats in the model control group were anesthetized and treated with MACO for 7 days after the rats were fed with distilled water of the same volume. The model of acute cerebral ischemia injury in rats was made after operation. After the rats were anesthetized and awake, the rats were gavaged with normal saline until all the time points were put to death. The rats in the Qingnao Yiyuan group were treated with the same model control group after 7 days of oral administration of the same volume of Qingnao Yiyuan. After the rats were anesthetized and awake, the rats were continued to be gavaged with traditional Chinese medicine to the death time points of the rats. According to the 5-point method of Longa, the neurological impairment score of rats was evaluated, and the specimens were collected according to the time points. The expression of VEGF in ischemic cortex and hippocampus was detected by real time PCR and Western blot was used to detect the expression of PDGF in ischemic cortex and hippocampus of rats. Results 1. The results of RT-PCR: compared with sham operation group, the expression of VEGF mRNA in brain tissue of model group was increased at all time points, but there was no significant difference between the two groups. Compared with model group, VEGF mRNA expression in Qingnao Yiyuan group was significantly increased at all time points. In Qingnao Yiyuan group, the expression of VEGF increased at the early stage (on the first day), increased obviously at the early stage (on the third day), and gradually increased to the peak on the 14th day, while in the model control group, the expression of VEGF increased slowly, and the difference was statistically significant (P 0.05). Results of Western blotting: compared with model group, PDGF expression in Qingnao Yiyuan group was significantly higher than that in model group. In Qingnao Yiyuan group, PDGF increased significantly at the early stage (day 1), but decreased slightly at the early stage (day 3), and reached its peak on the 14th day, and the expression of PDGF increased slowly in the model group. The difference was statistically significant (P 0.05). Conclusion: the protective effect of Qingnao Yiyuan decoction on neurons injured by cerebral ischemia may be related to the increase of VEGF PDGF expression.
【學(xué)位授予單位】：廣西中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R277.7

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 肖周華;陳雪梅;劉柏炎;;淺析治痰三法在中風(fēng)痰證中的辨證應(yīng)用[J];湖南中醫(yī)雜志;2015年03期

2 仲愛芹;徐士欣;張軍平;;從瘀毒論治缺血性中風(fēng)的理論探析[J];中華中醫(yī)藥學(xué)刊;2015年03期

3 靳建宏;白冠鋒;;補(bǔ)腎化痰逐瘀法治療缺血性中風(fēng)60例[J];中醫(yī)研究;2014年09期

4 陳澈;陳穎;周莉;高永紅;史永梅;劉培燕;高樹彪;張根明;;腦病痰濁病機(jī)源流探討[J];中國中醫(yī)基礎(chǔ)醫(yī)學(xué)雜志;2014年08期

5 金潔婷;楊金祿;;培元通腦膠囊治療腎元虧虛瘀血阻絡(luò)型缺血性腦中風(fēng)102例[J];上海中醫(yī)藥雜志;2014年05期

6 祝美珍;朱愛;蘇春壽;肖苗;胡躍強(qiáng);胡玉英;;加用清腦益元湯治療痰熱腑實(shí)型缺血性中風(fēng)療效觀察[J];廣西中醫(yī)藥;2014年02期

7 祝美珍;蘇春壽;吳志敏;齊玉潔;曾石森;朱愛;肖艷芬;;清熱化瘀Ⅱ號方對大鼠腦缺血再灌注損傷MMP-2及MMP-9表達(dá)的影響[J];中華中醫(yī)藥雜志;2014年02期

8 楊國忠;;中風(fēng)顆粒治療肝腎陰虛型缺血性中風(fēng)90例觀察[J];內(nèi)蒙古中醫(yī)藥;2014年02期

9 徐洛邦;王宜健;彭秀芳;;肉桂粉聯(lián)合常規(guī)治療脾腎陽虛證中風(fēng)后認(rèn)知功能障礙隨機(jī)平行對照研究[J];實(shí)用中醫(yī)內(nèi)科雜志;2013年24期

10 祝美珍;曾石森;齊玉潔;蘇春壽;朱愛;;基于中醫(yī)辨證論治思維體系的蛋白質(zhì)組學(xué)深入研究的思考[J];時珍國醫(yī)國藥;2013年12期

相關(guān)會議論文 前4條

1 張微微;徐風(fēng);楊華;蔡少青;葉加;;補(bǔ)陽還五湯多成分多靶點(diǎn)抗缺血性腦中風(fēng)機(jī)制研究[A];2013年中國藥學(xué)大會暨第十三屆中國藥師周論文集[C];2013年

2 王華;陳澤濤;;腎虛與缺血性中風(fēng)關(guān)系探討[A];第十三次全國中西醫(yī)結(jié)合虛證與老年醫(yī)學(xué)學(xué)術(shù)研討會論文集[C];2013年

3 張波;方媛媛;江婷戰(zhàn);黃為;劉妮;曹仕建;陳永華;王時光;李亞軍;;溫陽通脈顆粒治療脾腎陽虛型缺血性中風(fēng)的臨床研究[A];2012中國醫(yī)師協(xié)會中西醫(yī)結(jié)合醫(yī)師分會神經(jīng)病學(xué)專家委員會學(xué)術(shù)年會論文匯編[C];2012年

4 陳學(xué)忠;尹方;蘇凱;馬界;;缺血性中風(fēng)防治的切入點(diǎn)[A];中國中西醫(yī)結(jié)合學(xué)會養(yǎng)生學(xué)與康復(fù)醫(yī)學(xué)專業(yè)委員會委員會議暨第七次學(xué)術(shù)研討會論文集[C];2011年

相關(guān)博士學(xué)位論文 前4條

1 白永生;Fibulin-5對大鼠缺血再灌注腦損傷后腦水腫和軸突再生的影響及機(jī)制研究[D];重慶醫(yī)科大學(xué);2014年

2 唐璐;從HPA軸水平初步探討缺血性中風(fēng)急性期補(bǔ)腎護(hù)腦法的生物學(xué)基礎(chǔ)[D];北京中醫(yī)藥大學(xué);2011年

3 陳國華;黃角顆粒抗缺氧缺血神經(jīng)元細(xì)胞凋亡作用的實(shí)驗(yàn)研究[D];湖北中醫(yī)學(xué)院;2008年

4 汪棟材;糖尿病性腦梗塞中醫(yī)辨證論治研究[D];廣州中醫(yī)藥大學(xué);2001年

相關(guān)碩士學(xué)位論文 前10條

1 徐偉;益氣活血法與補(bǔ)腎生髓法對腦缺血再灌注大鼠額頂葉皮質(zhì)Notch信號轉(zhuǎn)導(dǎo)通路基因及差異蛋白表達(dá)的影響[D];安徽中醫(yī)藥大學(xué);2014年

2 劉政偉;馬云枝教授治療缺血性中風(fēng)臨床經(jīng)驗(yàn)總結(jié)[D];河南中醫(yī)學(xué)院;2014年

3 彭科;宮洪濤教授治療缺血性中風(fēng)的學(xué)術(shù)經(jīng)驗(yàn)研究[D];河南中醫(yī)學(xué)院;2014年

4 趙福臣;溫腎益氣活血法治療缺血性中風(fēng)病痙攣性偏癱的臨床研究[D];山東中醫(yī)藥大學(xué);2013年

5 于海默;益腎活血化痰法預(yù)防缺血性中風(fēng)后認(rèn)知障礙的小樣本臨床研究[D];北京中醫(yī)藥大學(xué);2013年

6 胡玉Z，

本文編號：2035891