本文選題：野菊花 + 濕疹��； 參考：《成都中醫(yī)藥大學》2016年碩士論文

【摘要】：研究目的：觀察野菊花不同提取物對于濕疹小鼠皮膚的干預(yù)作用,初探野菊花藥物治療濕疹的藥效及相關(guān)免疫機制。研究方法：將56只清潔級KM小鼠隨機平均分為空白組、模型組、陽性對照組(兒膚康搽劑組)、野菊花水提物組、95%、70%及50%濃度乙醇提物組,每組8只,采用2,4一二硝基氯苯(DNCB)腹部致敏,右耳部激發(fā)方法建立濕疹小鼠模型,同時于致敏第一天(d1)開始給予各組相應(yīng)藥物外用。末次激發(fā)后24h(d12)處死小鼠,收集小鼠血清及右耳耳片組織,測量小鼠右耳耳片腫脹度并在光鏡下觀察組織病理變化：采用高效液相色譜指紋圖譜(HPLC)建立野菊花四種提取物指紋圖譜,最后對空白組、模型組、陽性對照組及野菊花四組提取物中兩藥效及化合物成分優(yōu)異的野菊花水提物組和70%醇提取物組采用酶聯(lián)免疫吸附測定(Elisa)檢測小鼠血清免疫球蛋白E (IgE)水平,免疫組化檢測小鼠激發(fā)耳廓組織中白細胞介素-4(IL-4)、干擾素γ(IFN-γ)平均光密度值。研究結(jié)果：1、與空白組相較,模型組右耳部激發(fā)處出現(xiàn)明顯紅斑,水腫,少數(shù)皮膚可見糜爛、滲出結(jié)痂,同時光鏡下符合濕疹病理表現(xiàn)；組織切片腫脹度明顯增加,具有顯著差異(P0.01),陽性對照組(兒膚康)與野菊花四種提取物組小鼠耳片腫脹值結(jié)果均小于模型組,其結(jié)果具有顯著的統(tǒng)計學差異(P0.01),各組均能不同程度地改善激發(fā)右耳組織肉眼及病理學改變；野菊花水提取物組優(yōu)于野菊花不同比例醇提取物(P0.01),與陽性藥物療效作用相當(P0.05)；2、成功建立野菊花提取物高效液相指紋圖譜,圖譜顯示野菊花水提物主成分相對于醇提取物更為豐富,兩者內(nèi)均具有特殊波峰呈現(xiàn),70%組醇提取物在各醇提物主峰表現(xiàn)中波峰最為全面；3、與空白組相較,濕疹模型小鼠激發(fā)右耳組織IL-4平均光密度值明顯升高(P0.01),IFN-γ水平表達明顯降低(P0.01),模型血中IgE水平明顯升高(P0.01)；較模型組而言野菊花水提物可降低小鼠血清IgE水平(P0.05),顯著降低皮損中IL-4因子的表達(P0.01),同時能夠上調(diào)IFN-γ含量(P0.05)；而野菊花70%醇提取物組較模型組而言能顯著升高IFN-γ(P0.01),下調(diào)小鼠濕疹皮損中的IL-4水平(P0.05),具有降低血清IgE的趨勢(P0.05)。研究結(jié)論：1、野菊花藥物具有治療濕疹的作用,有效成分可能多存在于水提物及70%醇提取物的主要組分之中；2、野菊花能通過免疫調(diào)節(jié)改善濕疹小鼠TH1/TH2免疫功能失衡現(xiàn)象,其機制與抑制IL-4表達及提升IFN-γ的分泌相關(guān),不同提取物間存在著一定的作用差異；水提物能有效抑制IgE水平。
[Abstract]:Objective: To observe the effect of different extracts of chrysanthemum chrysanthemum on the skin of eczema in mice and explore the efficacy and immune mechanism of Chrysanthemum in the treatment of eczema. Methods: 56 clean KM mice were randomly divided into blank group, model group, positive control group (ER Fukang liniment group), water extracts of Chrysanthemum chrysanthemum, 95%, 70% and 50%. In the ethanol extract group, 8 rats in each group were treated with 2,4 one or two nitrochlorobenzene (DNCB) abdominal sensitization, the right ear excitation method was used to establish the eczema mouse model. At the same time, the first day (D1) was used to give the corresponding drug external use. After the last excitation, 24h (D12) was executed in mice, the mice were collected and the right ear ear slices were collected to measure the swelling degree of the right ear ear slices in mice. The histopathological changes were observed under light microscope: the fingerprint of four extracts of chrysanthemum Chrysanthemi was established by high performance liquid chromatography (HPLC). Finally, the enzyme linked immunosorbent assay was used in the blank group, model group, positive control group and four group of chrysanthemum chrysanthemum extract, which had two effective and excellent compounds in chrysanthemum and 70% alcohol extract group. The level of serum immunoglobulin E (IgE) in mice was detected by adsorption (Elisa). The average optical density of interleukin -4 (IL-4) and interferon gamma (IFN- gamma) in the auricle tissue of mice was detected by immunohistochemistry. The results were as follows: 1, compared with the blank group, there was obvious erythema, edema, erosion and crusting in the right ear of the model group. At the same time, the pathological features of eczema were conformed to the pathological features of the eczema, and the swelling degree of tissue sections increased significantly (P0.01). The results of the ear swelling of the four extracts of the positive control group (ER Fukang) and the chrysanthemum chrysanthemum were less than the model group. The results showed significant statistical difference (P0.01). All the groups could improve the excite right ear in varying degrees. The water extract group of chrysanthemum chrysanthemum was better than the alcohol extract of wild chrysanthemum (P0.01), and the effect was similar to that of the positive drug (P0.05). 2, the HPLC fingerprint of the extract of chrysanthemum chrysanthemum was successfully established. The map showed that the main component of the aqueous extract of Chrysanthemum Chrysanthemum was more abundant than the alcohol extract. Both of them had special characteristics. The peak of the 70% groups of alcohol extracts in the main peak of each alcohol extract was the most comprehensive. 3, compared with the blank group, the average light density of IL-4 in the right ear tissue of the eczema model mice increased significantly (P0.01), the expression of IFN- gamma decreased significantly (P0.01), and the level of IgE in the model blood was significantly increased (P0.01); compared with the model group, the water extraction of wild chrysanthemum was more than that of the model group. The serum IgE level (P0.05) was reduced and the expression of IL-4 factor in skin lesions (P0.01) was significantly reduced, and the content of IFN- gamma (P0.05) was up-regulated, while the 70% alcohol extract group of Chrysanthemum Chrysanthemum could significantly increase IFN- gamma (P0.01) and reduce the IL-4 level (P0.05) in the skin lesions of mice (P0.05), and had a tendency to reduce IgE in the serum (P0.05). Conclusion: 1, the drug of chrysanthemum chrysanthemum has the effect of treating eczema. The effective components may exist in the main components of water extract and 70% alcohol extract. 2, wild chrysanthemum can improve the immune function imbalance of TH1/TH2 in the eczema mice by immunoregulation. The mechanism is related to the inhibition of the expression of IL-4 and the secretion of IFN- gamma. Water extracts can effectively inhibit the level of IgE.
【學位授予單位】：成都中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R275.9

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