本文選題：失眠 + 下丘腦-垂體-腎上腺軸��； 參考：《成都中醫(yī)藥大學(xué)》2016年碩士論文

【摘要】：目的：觀(guān)察電針“神門(mén)”、“三陰交”對(duì)CRH注射失眠模型大鼠睡眠-覺(jué)醒、情緒狀態(tài)以及HPAA功能的影響,揭示電針治療失眠的中樞機(jī)制和關(guān)鍵性神經(jīng)遞質(zhì)。方法：將健康清潔級(jí)SD大鼠根據(jù)體重及曠場(chǎng)實(shí)驗(yàn)檢測(cè)篩選出合格的實(shí)驗(yàn)大鼠,并將合格的實(shí)驗(yàn)大鼠隨機(jī)分為空白組、假注射組、CRH模型組、CRH電針組。對(duì)CRH模型組、CRH電針組大鼠采取側(cè)腦室注射CRH法建立失眠模型,假注射組只在側(cè)腦室插入注射針頭但不給藥。側(cè)腦室注射后進(jìn)行24小時(shí)晝夜自發(fā)活動(dòng)及曠場(chǎng)實(shí)驗(yàn)檢測(cè)。檢測(cè)完成后對(duì)CRH電針組進(jìn)行電針“神門(mén)”、“三陰交”治療,每晚20：00開(kāi)始治療,1次/天,連續(xù)治療4天。治療后對(duì)各組大鼠進(jìn)行24小時(shí)晝夜自發(fā)活動(dòng)、曠場(chǎng)實(shí)驗(yàn)檢測(cè)。行為學(xué)檢測(cè)結(jié)束后,取股動(dòng)脈血以及丘腦、腦干,采用酶聯(lián)免疫法檢測(cè)各組大鼠外周血漿ACTH、CORT、5-HT和中樞CRH、5-HT含量。結(jié)果：1.24小時(shí)晝夜自發(fā)活動(dòng)：①經(jīng)側(cè)腦室注射后：白天活動(dòng)量：CRH模型組、CRH電針組大鼠24小時(shí)晝夜自發(fā)活動(dòng)白天活動(dòng)量與空白組相比明顯增多,均具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRH模型組、CRH電針組大鼠24小時(shí)晝夜自發(fā)活動(dòng)白天活動(dòng)量與假注射組相比明顯增多,均具有統(tǒng)計(jì)學(xué)意義(P0.05)；夜晚活動(dòng)量：假注射組大鼠24小時(shí)晝夜自發(fā)活動(dòng)夜晚活動(dòng)量與空白組相比略有減少,具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRH模型組、CRH電針組大鼠24小時(shí)晝夜自發(fā)活動(dòng)夜晚活動(dòng)量與空白組相比明顯減少,均具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRH模型組、CRH電針組大鼠24小時(shí)晝夜自發(fā)活動(dòng)夜晚活動(dòng)量與假注射組相比明顯減少,均具有統(tǒng)計(jì)學(xué)意義(P0.05)；②治療后：白天活動(dòng)量：CRH模型組大鼠白天活動(dòng)量與空白組相比明顯增多,CRH電針組大鼠電針治療后白天活動(dòng)量與空白組相比略有增多,均具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRH模型組大鼠白天活動(dòng)量與假注射組相比明顯增多,CRH電針組大鼠電針治療后白天活動(dòng)量與假注射組相比略有增多,均具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRH電針組大鼠電針治療后白天活動(dòng)量與CRH模型組相比明顯減少,具有統(tǒng)計(jì)學(xué)意義(P0.05)；夜晚活動(dòng)量：CRH模型組大鼠夜晚活動(dòng)量與空白組相比明顯減少,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRH模型組大鼠夜晚活動(dòng)量與假注射組相比明顯減少,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)：CRH電針組大鼠電針治療后夜晚活動(dòng)量與CRH模型組相比明顯增多,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。2.曠場(chǎng)實(shí)驗(yàn)：①基線(xiàn)：各組曠場(chǎng)實(shí)驗(yàn)外周格得分、中央格得分、垂直得分均采用單因素方差分析,差異沒(méi)有統(tǒng)計(jì)學(xué)意義,說(shuō)明基線(xiàn)曠場(chǎng)實(shí)驗(yàn)檢測(cè)指標(biāo)結(jié)果均衡,具有可比較性。②經(jīng)側(cè)腦室注射后：外周格得分：CRH模型組、CRH電針組外周格得分與空白組相比明顯減少,均具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRH模型組、CRH電針組外周格得分與假注射組相比明顯減少,均具有統(tǒng)計(jì)學(xué)意義(P0.05)；中央格得分：CRH模型組中央格得分與空白組相比增多,CRH電針組中央格得分與空白組相比明顯增多,均具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRH電針組中央格得分與假注射組相比略有增多,具有統(tǒng)計(jì)學(xué)意義(P0.05)；垂直得分：CRH模型組、CRH電針組垂直得分與空白組相比明顯增多,均具有統(tǒng)計(jì)學(xué)意義(P0.05)：CRH模型組、CRH電針組垂直得分與假注射組相比明顯增多,均具有統(tǒng)計(jì)學(xué)意義(P0.05)：③治療后：外周格得分：CRH模型組大鼠曠場(chǎng)外周格得分與空白組相比明顯減少,CRH電針組大鼠電針治療后曠場(chǎng)外周格得分與空白組相比略有減少,均具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRH模型組大鼠曠場(chǎng)外周格得分與假注射組相比明顯減少,CRH電針組大鼠電針治療后曠場(chǎng)外周格得分與假注射組相比略有減少,均具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRH電針組大鼠電針治療后外周格得分與CRH模型組相比明顯增多,具有統(tǒng)計(jì)學(xué)意義(P0.05)；中央格得分：CRH模型組大鼠中央格得分與空白組相比明顯增多,CRH電針組大鼠電針治療后中央格得分與空白組相比略有增多,均具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRH模型組大鼠中央格得分與假注射組相比明顯增多,CRH電針組大鼠電針治療后中央格得分與假注射組相比略有增多,均具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRH電針組大鼠電針治療后中央格得分與CRH模型組相比明顯減少,具有統(tǒng)計(jì)學(xué)意義(P0.05)；垂直得分：CRH模型組大鼠曠場(chǎng)垂直得分與空白組相比明顯增多,CRH電針組大鼠電針治療后曠場(chǎng)垂直得分與空白組相比略有增多,均具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRH模型組大鼠曠場(chǎng)垂直得分與假注射組相比明顯增多,CRH電針組大鼠電針治療后曠場(chǎng)垂直得分與假注射組相比略有增多,均具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRH電針組大鼠電針治療后垂直得分與CRH模型組相比明顯減少,具有統(tǒng)計(jì)學(xué)意義(P0.05)：3.血漿神經(jīng)遞質(zhì)含量：①CRH模型組大鼠外周血漿中5-HT、ACTH、CORT量與空白組、假注射組相比均明顯增多,差異均具有統(tǒng)計(jì)學(xué)意義(P0.05)；②CRH電針組大鼠電針治療后外周血漿中5-HT、ACTH、CORT量與CRH模型組相比均明顯減少,差異均具有統(tǒng)計(jì)學(xué)意義(P0.05)4.中樞神經(jīng)遞質(zhì)含量：①CRH模型組大鼠丘腦、腦干神經(jīng)遞質(zhì)CRH含量與空白組、假注射組相比均明顯增多,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRH電針組大鼠電針治療后丘腦、腦干神經(jīng)遞質(zhì)CRH含量與CRH模型組相比明顯減少,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)；②CRH模型組大鼠丘腦、腦干神經(jīng)遞質(zhì)5-HT含量較空白組、假注射組相比均明顯減少,差異均具有統(tǒng)計(jì)學(xué)意義(P0.05)；CRH電針組大鼠電針治療后丘腦、腦干神經(jīng)遞質(zhì)5-HT含量較CRH模型組明顯增多,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論：側(cè)腦室注射CRH可造成失眠模型,失眠模型大鼠下丘腦-垂體-腎上腺軸興奮性增高；電針“神門(mén)”、“三陰交”通過(guò)調(diào)整下丘腦-垂體-腎上腺軸的過(guò)度興奮治療失眠,下丘腦室旁核CRH是電針治療失眠癥的關(guān)鍵物質(zhì)。
[Abstract]:Objective: To observe the effects of "Shen men" and "Sanyinjiao" on sleep - Awakening, emotional state and HPAA function of CRH rats injected with insomnia model, and to reveal the central mechanism and key neurotransmitters of electroacupuncture in the treatment of insomnia. Methods: the healthy and clean SD rats were screened out of the qualified experimental rats according to the test of body weight and open field test. The qualified experimental rats were randomly divided into blank group, false injection group, CRH model group and CRH electroacupuncture group. In the CRH model group, the rats in the CRH electroacupuncture group were injected with the lateral ventricle with CRH to establish the insomnia model. The false injection group only inserted the injection needle in the lateral ventricle, but did not give the drug. The lateral ventricle was injected after 24 hours of spontaneous activity and open field test. After the test was completed, the CRH electroacupuncture group was treated by Electroacupuncture of "Shen gate" and "Three Yin cross" treatment. The treatment was started at 20:00 every night, 1 times a day and 4 days. After the treatment, the spontaneous activities of 24 hours and the open field test were carried out for the rats in each group. After the test of behavior, the blood of the femoral artery and the thalamus, brain stem were taken and the enzyme linked immunosorbent assay was used to detect each group. Rat peripheral plasma ACTH, CORT, 5-HT and central CRH, 5-HT content. Results: 1.24 hours of day and night spontaneous activity: (1) after the lateral ventricle injection: daytime activity: CRH model group, CRH electroacupuncture group 24 hours day and night spontaneous activity in day and night activity increased significantly compared with the blank group, all with statistical significance (P0.05); CRH model group, CRH electroacupuncture. The day and night activity of the rats in the 24 hours was significantly higher than that in the false injection group (P0.05), and the night activity of the rats in the 24 hour day and night decreased slightly compared with the blank group (P0.05), and the CRH model group and the CRH electroacupuncture group had 24 hours of day. The night activity of spontaneous night activity decreased significantly compared with that of the blank group (P0.05). CRH model group, the night activity of spontaneous activity in the 24 hour day and night of rats in the CRH electroacupuncture group was significantly lower than that of the false injection group (P0.05). (2) the daytime activity after treatment: the daytime activity of the CRH model group rats Compared with the blank group, the amount of daytime activity in the CRH electroacupuncture group increased slightly compared with that of the blank group (P0.05), and the amount of daytime activity in the CRH model group was significantly higher than that in the false injection group. The daytime activity of the electroacupuncture group of the CRH electroacupuncture group was slightly more than that of the false injection group. There was statistical significance (P0.05); in the CRH electroacupuncture group, the daytime activity after electroacupuncture was significantly lower than that of the CRH model group, with statistical significance (P0.05); the night activity in the CRH model group decreased significantly compared with the blank group in the CRH model group, and the difference had the statistical significance (P0.05); the activity of night activity in the CRH model group and the rats in the CRH model group were significantly higher than those in the CRH model group. Compared with the false injection group, the difference was statistically significant (P0.05) in the:CRH electroacupuncture group, the activity of electroacupuncture at night was significantly higher than that of the CRH model group. The difference was statistically significant (P0.05).2. open field experiment: (1) baseline: the score of the outer circumference, the score of the central lattice and the vertical score were all single factor. Difference analysis, the difference was not statistically significant, indicating that the results of the baseline field test were balanced and comparable. After the injection of the lateral ventricle, the score of peripheral blood in the CRH model group, the peripheral peripheral score of the CRH electroacupuncture group was significantly lower than that in the blank group, and all had statistical meaning (P0.05); the CRH model group, the peripheral peripheral score of CRH electroacupuncture group were scored and Compared with the false injection group, the score of the central lattice was statistically significant (P0.05). The score of central grid in the CRH model group was more than that in the blank group. The score of the central grid in the CRH electroacupuncture group was significantly higher than that in the blank group. All the scores were statistically significant (P0.05). The score of the central grid in the CRH electroacupuncture group was slightly more than that of the false injection group. Study significance (P0.05); vertical score: CRH model group, the vertical score of CRH electroacupuncture group was significantly increased compared with the blank group, all had statistical significance (P0.05):CRH model group, the vertical score of CRH electroacupuncture group was significantly increased compared with the false injection group (P0.05): after treatment: the peripheral score: CRH model group rats open. Compared with the blank group, the score of the OTC week in the CRH electroacupuncture group was significantly lower than that in the blank group (P0.05). The score of the open field week in the CRH model group was significantly lower than that in the false injection group. The score of the open field week after the electroacupuncture group of the CRH electroacupuncture group was compared with that of the false injection group. Compared with the CRH model group, the score of the rats in the CRH electroacupuncture group was significantly higher than that of the CRH model group (P0.05), and the central grid score of the CRH model group was significantly higher than that in the blank group, and the central grid in the CRH model group was significantly higher than that in the blank group, and the center of the CRH electroacupuncture group was in the center of the electroacupuncture group after the treatment. The score of the grid was a slight increase compared with that in the blank group (P0.05); the score of the central grid in the CRH model group was significantly higher than that in the false injection group. The score of the central grid in the electroacupuncture group of the CRH electroacupuncture group was slightly higher than that in the false injection group, and all had statistical meaning (P0.05), and the central lattice of the electroacupuncture group of the CRH electroacupuncture group after the electroacupuncture treatment. The score was significantly lower than that of the CRH model group (P0.05), and the vertical score of the rats in the CRH model group was significantly higher than that in the blank group. The vertical score of the open field after the electroacupuncture group of the CRH electroacupuncture group was slightly increased compared with the blank group (P0.05), and the open field of the CRH model group was perpendicular to the open field. The scores of the rats in the CRH electroacupuncture group increased slightly compared with those in the false injection group, and there was a statistical significance (P0.05). The vertical score of the electroacupuncture group in the CRH electroacupuncture group was significantly lower than that in the CRH model group, with a statistically significant (P0.05):3. plasma neurotransmitter content: (1) the amount of 5-HT, ACTH, CORT in the peripheral plasma of the CRH model group was significantly increased with the blank group, and the difference was statistically significant (P0.05). (2) the plasma 5-HT, ACTH, CORT in the peripheral plasma of the CRH electroacupuncture group were significantly decreased compared with the CRH model group, and the difference was statistically significant (P0.05) 4. central nerve delivery. Content: (1) CRH model group rats thalamus, brain stem neurotransmitter CRH content and blank group, compared with the false injection group was significantly increased, the difference was statistically significant (P0.05); CRH electroacupuncture group rats after electroacupuncture treatment of thalamus, brainstem neurotransmitter CRH content compared with the CRH model group significantly decreased, the difference was statistically significant (P0.05); (2) CRH model The content of 5-HT in the thalamus and brainstem neurotransmitter in the rat model group was significantly lower than that in the blank group, and the difference was statistically significant (P0.05). The content of 5-HT in the thalamus and brainstem neurotransmitter in the CRH electroacupuncture group was significantly higher than that in the CRH model group, and the difference was statistically significant (P0.05). Conclusion: the lateral ventricle injection of CRH can be made. As a model of insomnia, the hypothalamus pituitary adrenal axis excitability of the rat model of insomnia is increased; Electroacupuncture "Shen gate" and "Three Yin" are used to treat insomnia by adjusting the hyperexcitation of the hypothalamus pituitary adrenal axis. The paraventricular nucleus CRH of the inferior colliculus is the key substance of electroacupuncture in the treatment of insomnia.
【學(xué)位授予單位】：成都中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類(lèi)號(hào)】：R245.97;R-332

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