本文選題：對(duì)葉大戟 + 質(zhì)量評(píng)價(jià)　； 參考：《新疆師范大學(xué)》2016年碩士論文

【摘要】：目的:對(duì)葉大戟植物記載于《中國(guó)植物志》第44(3)卷,其中只收錄了對(duì)葉大戟的名稱、性狀、產(chǎn)地、來(lái)源、鑒別。衛(wèi)生部頒標(biāo)準(zhǔn)《維藥分冊(cè)》(31)收錄了對(duì)葉大戟果性狀、鑒別、雜質(zhì)和水分檢查、功能與主治。對(duì)葉大戟現(xiàn)階段未有相應(yīng)的質(zhì)量標(biāo)準(zhǔn)要求,通過對(duì)葉大戟藥材化學(xué)成分及質(zhì)量標(biāo)準(zhǔn)進(jìn)行科學(xué)研究,能更好的開發(fā)這一種新疆特色維吾爾醫(yī)藥材。方法:化學(xué)成分系統(tǒng)預(yù)試法初步判斷對(duì)葉大戟藥材中的化學(xué)成分種類;10批對(duì)葉大戟藥材中水分、浸出物、總灰分、酸不溶性物質(zhì)進(jìn)行測(cè)定。根據(jù)2015版《中國(guó)藥典》要求,采用ICP-AES法測(cè)定Fe、Cu、Zn、Cr;石墨爐原子吸收法測(cè)定Pb和Cd.;原子熒光法測(cè)定As和Hg進(jìn)行含量測(cè)定。利用紫外可見分光光度儀測(cè)定總多酚、總黃酮的含量。采用紅外光譜和化學(xué)計(jì)量學(xué)分析方法對(duì)10批對(duì)葉大戟藥材及其提取物的紅外光譜規(guī)律進(jìn)行探究。HPTLC法對(duì)10批對(duì)葉大戟藥材中3種有效成分山奈酚、莨菪亭、香草酸進(jìn)行定性研究;采用HPLC測(cè)定對(duì)葉大戟中三種有效成分含量。結(jié)果:化學(xué)成分系統(tǒng)預(yù)試結(jié)果表明對(duì)葉大戟化學(xué)成分主要含有黃酮類、酚類、糖類、生物堿等.10批對(duì)葉大戟藥材水分、總灰分、水溶性浸出物和醇溶性浸出物平均值分別為:5.74%、11.53%、17.68%、14.82%;采用ICP-AES、石墨爐原子吸收、原子熒光測(cè)定10批對(duì)葉大戟中8種規(guī)定元素(Fe、Cu、Zn、Cr、Pb、Cd、As、Cr)的含量,藥材中8種元素含量均符合2015版《中國(guó)藥典》元素含量要求;通過對(duì)10批對(duì)葉大戟提取液中總多酚、總黃酮進(jìn)行提取工藝優(yōu)化,得到總多酚和總黃酮最佳提取工藝條件分別是:乙醇濃度70%、料液比1:40、溫度45℃;超聲時(shí)間30 min和乙醇濃度70%、料液比1:35、溫度35℃、超聲時(shí)間40 min;含量分別在9.89-14.33和18.01-23.14 mg/g之間。10批對(duì)葉大戟中紅外吸收峰的峰數(shù)、峰位、峰形、峰強(qiáng)度存在一定的差異,通過對(duì)樣品紅外光譜的比較,峰:3391cm-1、2927cm-1、1324cm-1、1240cm-1可作為對(duì)葉大戟藥材共有特征峰,采用主成分分析和聚類分析法將10批對(duì)葉大戟進(jìn)行分類;10批對(duì)葉大戟中山奈酚、莨菪亭和香草酸成分薄層分離效果較好,可作為對(duì)葉大戟質(zhì)量標(biāo)準(zhǔn)評(píng)價(jià)的一個(gè)指標(biāo)。建立了HPLC法測(cè)定對(duì)葉大戟中山奈酚、莨菪亭、香草酸含量,為對(duì)葉大戟藥用提供了理論依據(jù)。結(jié)論:本研究初步探索了維藥對(duì)葉大戟的質(zhì)量評(píng)價(jià)方法,建立了對(duì)葉大戟中香草酸、莨菪亭、山奈酚的含量測(cè)定方法,測(cè)定了對(duì)葉大戟中不同的元素含量,為維藥對(duì)葉大戟質(zhì)量評(píng)價(jià)研究提供科學(xué)依據(jù)。
[Abstract]:Objective: halberd was recorded in the Chinese Flora 443, which contains only the name, character, origin, origin and identification of Euphorbia. The standard issued by the Ministry of Health, "Veterinary Medicine Partition", includes the characters, identification, impurity and moisture examination, function and main treatment of Euphorbia esculentum. There is no corresponding quality standard for Euphorbia esculentata at the present stage. Through the scientific research on the chemical composition and quality standard of Euphorbia esculentata, this kind of Uygur medical material with Xinjiang characteristics can be better developed. Methods: 10 batches of chemical constituents of Euphorbia esculentata were determined by the method of chemical composition system pretest. The contents of water, extract, total ash and acid insoluble substances in Euphorbia esculentata were determined in 10 batches. According to the requirements of Chinese Pharmacopoeia 2015 edition, ICP-AES method was used to determine the content of FebCuCuznzncr, graphite furnace atomic absorption spectrometry (GFAAS) to determine Pb and Cd.and atomic fluorescence spectrometry (AFS) to determine the contents of as and Hg. The contents of total polyphenols and total flavonoids were determined by UV-vis spectrophotometer. Infrared spectroscopy and chemometrics were used to study the infrared spectra of 10 batches of Euphorbia esculentata and its extracts. HPTLC was used to qualitatively study the three active components of Euphorbia mongolicum, scopoletin and vanillic acid in 10 batches of Euphorbia officinalis. The content of three active components in Euphorbia davidiana was determined by HPLC. Results: the chemical composition system showed that the chemical constituents of Euphorbia esculentum mainly contained flavonoids, phenols, carbohydrates, alkaloids and so on. The average values of water-soluble extracts and alcohol-soluble extracts were 11.53 and 17.68, respectively. ICP-AES, graphite furnace atomic absorption spectrometry and atomic fluorescence spectrometry were used to determine the contents of 8 specified elements in 10 batches of Euphorbia trifoliata. The total polyphenols and flavonoids in 10 batches of Euphorbia lobata extract were optimized by the content of 8 elements in the 2015 edition of Chinese Pharmacopoeia, and the contents of 8 elements in the medicinal materials met the requirements of the 2015 edition of the Chinese Pharmacopoeia. The optimum extraction conditions of total polyphenols and total flavonoids were as follows: ethanol concentration 70, ratio of material to liquid 1: 40, temperature 45 鈩，

本文編號(hào)：1902116