本文選題：腫瘤 + 肺瘤平膏�。� 參考：《中醫(yī)雜志》2017年01期

【摘要】：目的探討肺瘤平膏抗腫瘤的可能免疫調節(jié)機制。方法肺瘤平膏拆方分為解毒方、益氣方、益氣解毒方,分別制備含藥血清。應用掃描電鏡及激光共聚焦顯微鏡觀察成熟樹突狀細胞與T細胞體外相互作用動態(tài)過程。樹突狀細胞培養(yǎng)設為空白組,將負載腫瘤抗原樹突狀細胞分為負載組、解毒組、益氣組、益氣解毒組、全方組和地米低、中、高劑量組,每組設定106/106、106/105、105/106、105/105(樹突狀細胞/T細胞)4個濃度配比。細胞培養(yǎng)第2天,解毒組、益氣組、益氣解毒組、全方組吸取相應中藥血清1 ml加入細胞中,地米低、中、高劑量組分別加入濃度為37.5、75.0、150.0 mg/ml地塞米松磷酸鈉注射液1 ml,空白組、負載組加入不完全RPMI-1640培養(yǎng)液1 ml。于培養(yǎng)第8天,比較各組在混合培養(yǎng)3、6 h時T細胞數和免疫突觸數。結果體外負載腫瘤抗原樹突狀細胞與T細胞體外混合培養(yǎng)30 min時觀察到免疫突觸形成。3 h時T細胞發(fā)生了明顯增殖,6 h后隨著免疫突觸的減少,細胞分布逐漸趨于均勻。全方組在3、6 h時T細胞數和免疫突觸數均明顯高于其余各組(P0.05)。各給藥組在106/106配比下免疫突觸數均明顯高于其他配比(P0.01)。各組細胞在不同配比下培養(yǎng)6 h與本組3 h時免疫突觸數比較差異均無統(tǒng)計學意義(P0.05)。結論肺瘤平膏具有一定調節(jié)免疫突觸形成及促進負載腫瘤抗原樹突狀細胞刺激T細胞增殖作用,且全方效果優(yōu)于其拆方。
[Abstract]:Objective to explore the possible immunomodulatory mechanism of Feiyangping ointment in anti-tumor. Methods Feiyangping ointment was divided into jiedu prescription and Yiqi jiedu prescription. The dynamic process of interaction between mature dendritic cells and T cells in vitro was observed by scanning electron microscope and laser confocal microscope. Dendritic cells were divided into three groups: loading group, detoxifying group, supplementing qi group, whole prescription group and low, medium and high dose groups. Each set a ratio of 106 / 106106 / 105105 / 106105 (dendritic cell / T cell). On the second day of cell culture, the detoxification group, Yiqi detoxification group, and the whole prescription group absorbed 1ml of serum from traditional Chinese medicine to be added into the cells. The concentration of dexamethasone sodium phosphate injection was 37.5 mg/ml 75.0150.0 mg/ml in the low, medium and high dose groups, respectively, and the blank group was blank group. The loading group added 1 ml of incomplete RPMI-1640 medium. On the 8th day, the number of T cells and the number of immunosynaptic synapses were compared. Results after 30 min of mixed culture of dendritic cells and T cells loaded with tumor antigen in vitro, it was observed that T cells proliferated significantly at 3. 3 h after immunosynaptic formation. After 6 h, the distribution of T cells became more and more uniform with the decrease of immune synapses. The number of T cells and the number of immunosynaptic synapses in the whole prescription group were significantly higher than those in the other groups at 3h. The number of immunosynaptic synapses in each group at 106 / 106 was significantly higher than that in other groups (P 0.01). There was no significant difference in the number of immunosynaptic synapses between the cells cultured in different proportions for 6 h and 3 h in this group (P 0.05). Conclusion HYP can regulate immune synapse formation and promote T cell proliferation stimulated by dendritic cells loaded with tumor antigen.
【作者單位】： 中國人民解放軍第三0六醫(yī)院;中國科學院國家納米中心;中國中醫(yī)科學院廣安門醫(yī)院;
【基金】：北京市自然科學基金(7122156)
【分類號】：R273

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