本文選題：舌苔微生物 + 肺癌��； 參考：《皖南醫(yī)學(xué)院》2016年碩士論文

【摘要】：背景:舌診是傳統(tǒng)中醫(yī)獨(dú)特的診斷方法,通過辨析觀察舌苔的顏色、腐膩、厚薄等不同中醫(yī)舌苔類型來判斷人體生理功能及病理情況。據(jù)現(xiàn)階段研究結(jié)果顯示,舌苔的形成機(jī)制目前尚未明確,而舌苔微生物是影響舌苔形成的重要原因。傳統(tǒng)微生物研究的分子生物學(xué)方法如熒光定量PCR、變性梯度凝膠電泳DGGE等不僅工作量大、耗時(shí)長,且檢測(cè)出的微生物豐度有限。Illumina Miseq測(cè)序技術(shù)彌補(bǔ)了傳統(tǒng)分子生物技術(shù)的缺陷,對(duì)舌苔微生物豐度和多樣性進(jìn)行了全面分析,滿足對(duì)舌苔微生物的全面認(rèn)識(shí)。肺癌是我國癌癥發(fā)病率和死亡率最高的腫瘤之一。目的:本課題采用Illumina Miseq測(cè)序平臺(tái)對(duì)肺癌患者不同中醫(yī)舌苔類型的舌苔微生物進(jìn)行分析,目的為探討肺癌患者不同中醫(yī)舌苔類型和其舌苔微生物結(jié)構(gòu)之間的關(guān)系及規(guī)律,進(jìn)一步揭示舌苔形成原理,促進(jìn)中醫(yī)舌診的客觀化和標(biāo)準(zhǔn)化。方法:收集2013年12月至2014年12月2皖南醫(yī)學(xué)院附屬弋磯山醫(yī)院腫瘤防治中心收治肺癌患者(均經(jīng)病理確診為肺癌者)41例,體檢中心招募健康正常人43例。所有入選對(duì)象均簽訂知情同意書并獲皖南醫(yī)學(xué)院倫理道德委員會(huì)同意。研究對(duì)象端坐舌象儀(DS01-B)前進(jìn)行舌象采集和分析后,用拭子采集舌苔并保存在EP管中留用。提取14名肺癌患者和7名健康人的舌苔樣本微生物的總DNA,16S r RNA基因V3-V4高變區(qū)經(jīng)PCR擴(kuò)增后產(chǎn)物進(jìn)行Illumina測(cè)序,對(duì)舌苔微生物的組成,豐度和多樣性進(jìn)行全面的分析。結(jié)果:1.經(jīng)舌象信息采集系統(tǒng)分析,41名肺癌患者中常見舌苔類型有薄白苔21例,白膩苔10例,黃膩苔8例,無苔2例;43例健康人中42人為薄白苔,1人為薄黃苔。肺癌患者舌苔厚度明顯高于健康人(314.86±222.47 vs.80.00±75.84,p0.01)。2.41例肺癌患者有8例厚苔和6例薄苔,43例健康人中7例,共21份樣本進(jìn)行后續(xù)舌苔微生物測(cè)序分析。對(duì)21份舌苔微生物樣本進(jìn)行Illumina測(cè)序共獲得508,003個(gè)有效序列,總堿基數(shù)為224,746,932bp,平均444.26bp,其中99.99%的序列長度為401-500bp。3.稀釋性曲線表明本次測(cè)序覆蓋了舌苔微生物97%的菌種。多樣性分析提示厚苔組舌苔微生物豐度和多樣性較其他兩組均低。4.門水平,厚苔組檢測(cè)出12個(gè)菌門,薄苔組檢測(cè)出14個(gè)菌門,對(duì)照組19個(gè)菌門,與多樣性分析相符。三組中主要菌門為厚壁菌門Firmicutes、擬桿菌門Bacteriodetes、變形菌門Proteobateria、放線菌門Actinobacteria、梭桿菌Fusobacteria,占所有門類的95%以上。屬水平檢測(cè)厚苔組、薄苔組和健康對(duì)照組分別有132,129和123個(gè)菌屬。三組中主要菌屬有普氏菌Prevotella、鏈球菌Streptococcus、韋榮菌Veillonella、奈瑟菌Neisseria、乳酸球菌Lactococcus、流感嗜血桿菌Haemophilus、顆粒鏈菌Granulicatella and梭桿菌Fusobaterium。厚苔組優(yōu)勢(shì)菌有鏈球菌Streptococcus、乳酸球菌Lactococcus和放線菌Actinomyces。結(jié)論:1.舌苔微生物的組成可能與中醫(yī)舌苔類型有關(guān);2.肺癌患者舌苔微生物的多樣性和差異性可能是引起患者舌苔增厚的原因之一;3.特異性的舌苔微生物檢測(cè)可能成為肺癌早期診斷的特異性生物標(biāo)志物。
[Abstract]:Background: tongue diagnosis is a unique diagnostic method of traditional Chinese medicine. By distinguishing and observing the color of tongue coating, greasy, thick and thin tongue coating types to judge the physiological functions and pathological conditions of the human body. According to the results of the present stage, the mechanism of the formation of tongue fur is not yet clear, and the tongue coating microorganism is the important reason for the formation of tongue coating. Tradition The molecular biology methods, such as fluorescence quantitative PCR, denaturing gradient gel electrophoresis (DGGE) and so on, are not only large and time-consuming, but also the detected microbial abundance limited.Illumina Miseq sequencing technology makes up for the defects of traditional molecular biological technology, and makes a comprehensive analysis on the abundance and diversity of the tongue coating microorganism and satisfies the tongue fur. Lung cancer is one of the most common cancer incidence and mortality in China. Objective: the purpose of this study was to analyze the tongue coating microbes of different tongue coating types of lung cancer patients by Illumina Miseq sequencing platform, in order to explore the relationship between tongue coating types of different TCM and the structure of tongue coating microorganism in lung cancer patients. To further reveal the principle of the formation of tongue coating and to promote the objectification and standardization of tongue diagnosis in traditional Chinese medicine. Methods: 41 cases of lung cancer patients (all patients with lung cancer confirmed by pathology) were collected from December 2013 to December 2014, 2 of the cancer prevention and control center of Yi La Shan Hospital Affiliated to Wangnan Medical College, and 43 healthy people were recruited in the medical center. All the selected subjects were selected. They all signed the informed consent and received the consent of the ethics and moral Committee of Wangnan Medical College. After the study of tongue image acquisition and analysis, the object of the study was collected and analyzed by the tongue image instrument (DS01-B). The tongue coating was collected with swabs and retained in the EP tube. The total DNA of the tongue fur samples of 14 lung cancer patients and 7 healthy people was extracted, and the 16S R RNA gene V3-V4 high variation area was PCR. The amplified products were sequenced by Illumina and analyzed the composition, abundance and diversity of the tongue coating microorganism. Results: 1. the common tongue coating types in 41 lung cancer patients were thin white moss, 10 white and greasy fur, 8 yellow and greasy fur, 2 cases without moss, 42 thin white moss in 43 healthy people and 1 people with thin yellow moss. The thickness of tongue coating in cancer patients was significantly higher than that of healthy people (314.86 + 222.47 vs.80.00 + 75.84, P0.01). There were 8 thick moss and 6 thin moss in.2.41 patients and 7 cases in 43 healthy people. 21 samples were sequenced in the following tongue coating microorganism. 508003 effective sequences were obtained by Illumina sequencing for 21 tongue coated microorganism samples. The total base number of the total base was the base number 224746932bp, with an average 444.26bp, 99.99% of the sequence length was 401-500bp.3. dilution curve, indicating that the sequence covered 97% of the tongue coating microorganism. The diversity analysis suggested that the abundance and diversity of the tongue fur of the thick moss group were lower than those of the other two groups, and 12 fungi were detected in the thick moss group, and 14 bacteria were detected in the thin moss group. The 19 bacteria gates in the control group were in accordance with the diversity analysis. The main bacteria in the three groups were Firmicutes, Bacteriodetes, Proteobateria, actinomycetes Actinobacteria, and Clostridium Fusobacteria, which accounted for more than 95% of all categories. The main bacteria in the three groups are Prevotella, Streptococcus Streptococcus, Wei Rongjun Veillonella, Neisseria Neisseria, Lactococcus lactis Lactococcus, Haemophilus influenzae Haemophilus, Streptococcus Fusobaterium. moss of Granulicatella and Clostridium of granular Streptococcus, Streptococcus lactis Lactococcus and actinomycetes. Actinomyces. conclusion: the composition of 1. tongue coating microbes may be related to the type of tongue coating of traditional Chinese medicine; 2. the diversity and difference of the tongue coating microorganism in the patients with lung cancer may be one of the reasons for the thickening of the tongue fur of the patients; the 3. specific tongue coating microorganism detection may be a specific biomarker for the early diagnosis of lung cancer.

【學(xué)位授予單位】：皖南醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R273

【相似文獻(xiàn)】

相關(guān)期刊論文 前2條

1 ;利用石油培養(yǎng)細(xì)菌制造蛋白可以滿足全人類蛋白營養(yǎng)的不足[J];骨科;1964年02期

2 ;[J];;年期

相關(guān)會(huì)議論文 前5條

1 莊鐵城;張瑜斌;;紅樹林微生物研究三十年[A];2008年中國微生物學(xué)會(huì)學(xué)術(shù)年會(huì)論文摘要集[C];2008年

2 趙立平;;微生物群落結(jié)構(gòu)多樣性的分子解析與種群動(dòng)態(tài)分子監(jiān)控研究進(jìn)展[A];2006中國微生物學(xué)會(huì)第九次全國會(huì)員代表大會(huì)暨學(xué)術(shù)年會(huì)論文摘要集[C];2006年

3 高小朋;任桂梅;張欠欠;;分子生物學(xué)技術(shù)在環(huán)境微生物研究中的應(yīng)用[A];第十次全國環(huán)境微生物學(xué)術(shù)研討會(huì)論文摘要集[C];2007年

4 毛元圣;曹曉清;王建華;吳瑞華;;烏梢蛇的寄生蟲和致病微生物研究[A];動(dòng)物學(xué)專輯——上海市動(dòng)物學(xué)會(huì)2002年年會(huì)論文集[C];2002年

5 黃小蘭;陳建耀;謝麗純;付叢生;周世寧;;小流域地下水流微生物空間差異分析[A];第八屆博士生學(xué)術(shù)年會(huì)論文摘要集[C];2010年

相關(guān)重要報(bào)紙文章 前10條

1 記者 付毅飛;中科院應(yīng)用微生物研究網(wǎng)絡(luò)啟動(dòng)[N];科技日?qǐng)?bào);2010年

2 任玉嶺;微生物：生態(tài)環(huán)境建設(shè)的主角[N];西安日?qǐng)?bào);2002年

3 辛偉強(qiáng);大慶局承擔(dān)國家863計(jì)劃項(xiàng)目獲重大突破[N];中國石油報(bào);2005年

4 本報(bào)記者 熊燕;微生物里的大千世界[N];云南日?qǐng)?bào);2004年

5 記者 翟穎娜　實(shí)習(xí)生 李卡;我市成立微生物協(xié)會(huì)[N];保定日?qǐng)?bào);2010年

6 記者 尚靜;我區(qū)發(fā)現(xiàn)世界首個(gè)耐高輻射放線菌和真菌[N];新疆科技報(bào)(漢);2010年

7 本報(bào)駐莫斯科記者 王江;和平站的另一種危險(xiǎn)[N];科技日?qǐng)?bào);2000年

8 本報(bào)特派記者 劉春曉;探索大洋微生物的“新邊疆”[N];中國海洋報(bào);2011年

9 林岳夫;海洋生物科研結(jié)碩果[N];中國海洋報(bào);2004年

10 記者 李斌;我科學(xué)家在地下2000米深處巖石里發(fā)現(xiàn)生命[N];新華每日電訊;2004年

相關(guān)碩士學(xué)位論文 前5條

1 陳妍;肺癌患者不同舌苔類型的舌苔微生物研究[D];皖南醫(yī)學(xué)院;2016年

2 王寧;污損早期微生物群落結(jié)構(gòu)的動(dòng)態(tài)變化的初步研究[D];哈爾濱工業(yè)大學(xué);2012年

3 趙s閿，

本文編號(hào)：1882043