本文選題：針刺 + 高尿酸血癥�。� 參考：《北京中醫(yī)藥大學》2017年碩士論文

【摘要】：研究目的:通過分別針刺大鼠肝、脾和腎的俞穴、原穴以及大、小腸的募穴這3組穴位,觀察不同的穴位對于痛風早期高尿酸血癥模型大鼠體內(nèi)的SUA、XOD、ALP含量和腎臟指數(shù)的影響,以及對于腎臟的大體形態(tài)和腎臟細胞組織結(jié)構(gòu)形態(tài)的影響,明確這3組針刺穴位對于痛風高尿酸血癥的形成是否具有干預預防的作用,篩選針刺防治痛風高尿酸血癥的最佳穴位,為臨床治療提供選穴依據(jù)。研究方法:將50只健康的、成年的雄性Wistar大鼠采取隨機分組的方法,平均分成5組:空白組、模型組、俞穴組、原穴組、募穴組,每組10只。造模采用灌胃給藥的方法制備高尿酸血癥大鼠模型,造模劑為腺嘌呤(100mg/kg)加乙胺丁醇(250mg/kg)。5組同時于實驗第1天開始造模,前21天每日灌胃一次造模劑,21天之后改為隔日灌胃一次,直到第90天實驗結(jié)束。其中的俞穴組、原穴組和募穴組3組針刺干預組于造模的同時予以針刺治療,隔日針刺1次,每次針刺15min,針刺10次作為1個療程,每療程結(jié)束后停止針刺10天,之后再進行下一療程的干預,如此循環(huán)往復,到實驗第90天結(jié)束,共3個療程。實驗結(jié)束后,5組大鼠統(tǒng)一取材,對于指標的檢測,實驗采用全自動生化分析儀檢測SUA及ALP,免疫熒光法檢測XOD,將大鼠腎臟做成石蠟切片,用HE染色法染色,再置于普通光學顯微鏡下觀察其細胞組織結(jié)構(gòu)。實驗結(jié)果:與空白組相比較,模型組的SUA和XOD含量明顯升高,腎臟指數(shù)也明顯增大,肉眼觀察腎臟體積增大,鏡下觀察腎臟切片腎小管擴張,炎性細胞浸潤;與模型組相比較,俞穴組、原穴組和募穴組3組針刺干預組的SUA、XOD含量均有不同程度的降低,腎臟指數(shù)和腎臟體積也均有減小,鏡下觀察腎臟HE染色切片腎小管擴張程度降低,炎性細胞數(shù)量減少,胞漿空泡減少;3組針刺干預組之間相互比較,腎臟指數(shù)方面組間并無明顯的差異性,募穴組、原穴組降低SUA、XOD含量的效果較俞穴組顯著,其中募穴組在降低SUA含量方面的干預效果最明顯;5組實驗組之間ALP含量均互無明顯的差異性。實驗結(jié)論:(1)3組針刺干預組均能在不同程度上降低SUA和XOD的含量,并且均能有效地降低對腎臟的損害,減緩造模劑對腎臟細胞的正常形態(tài)和功能的侵蝕過程,在一定程度上成功地預防了高尿酸血癥的形成,防止了腎損害,預防了痛風;(2)在降低SUA含量方面,干預效果的顯著程度以募穴組為最佳,原穴組的效果次之,俞穴組的效果在3組中相對最不明顯;在抑制X0D活性方面,募穴組和原穴組的干預效果要好于俞穴組;(3)5組實驗組的ALP含量互無差異性,說明體內(nèi)ALP含量的影響因素可能不僅是高尿酸血癥,同時也是體內(nèi)其他復雜變化綜合作用的結(jié)果,具體原因還需做進一步的研究。
[Abstract]:Objective: to observe the effects of different acupoints on the content of SUAXODN ALP and renal index in rats with early gout hyperuricemia by acupuncture the points of liver, spleen and kidney at Shu point, original point, large point and small intestine acupoint, respectively, in order to observe the effect of different acupoints on the content of SUAXODN ALP in rats with early gout hyperuricemia. The effects of acupuncture on the gross morphology of kidney and the tissue structure of renal cells were also discussed. Whether the acupuncture points of these three groups could interfere with and prevent the formation of hyperuricemia in gout was determined. To select the best acupuncture points for prevention and treatment of hyperuricemia of gout. Methods: 50 healthy adult male Wistar rats were randomly divided into 5 groups: blank group, model group, Shu group, original acupoint group and mu-point group, with 10 rats in each group. The rat model of hyperuricemia was established by intragastric administration of adenine (100 mg / kg) and ethylamine butanol (250 mg / kg). The model was established on the first day of the experiment. After 21 days of oral administration of the model agent once a day, the rat model was given once every other day. Until the end of the 90 th day. Among them, the group of Shu, the group of original acupoint and the group of acupoint raising were treated with acupuncture at the same time when the model was made. The acupuncture was treated once every other day for 15 mins each time, 10 times as a course of treatment, and 10 days after the end of each course of treatment, the acupuncture was stopped for 10 days. Then the next course of intervention, so cycle, to the end of the experiment 90 days, a total of 3 courses. SUA and ALP were detected by automatic biochemical analyzer, XODs were detected by immunofluorescence, and the kidneys were made into paraffin sections and stained by HE staining. The tissue structure of the cells was observed under the ordinary optical microscope. Results: compared with the blank group, the contents of SUA and XOD in the model group were significantly increased, the renal index was also significantly increased, the size of the kidney was increased with the naked eye, the renal tubular dilatation and inflammatory cell infiltration were observed under microscope, and compared with the model group, The content of SUAXOD, renal index and renal volume in the three groups of acupuncture intervention group were all decreased, and the renal tubule dilatation degree was decreased and the number of inflammatory cells was decreased in HE stained sections of kidney under microscope. There was no significant difference in renal index between the three acupuncture intervention groups. The effect of reducing the XOD content in the acupoint group and the original acupoint group was significantly higher than that in the Shu group, but there was no significant difference in the renal index between the acupuncture intervention groups and the original acupoint group, but there was no significant difference in the renal index between the three groups. There was no significant difference in ALP content among the five experimental groups. Conclusion Acupuncture intervention group can decrease the content of SUA and XOD in different degree, and can effectively reduce the damage to kidney and the erosion process of normal morphology and function of kidney cells. To a certain extent, the formation of hyperuricemia was prevented, kidney damage was prevented, and gout was prevented. In the aspect of reducing SUA content, the intervention effect was the best in the control group, followed by the original point group. In inhibiting X0D activity, the intervention effect of the acupoint raising group and the original point group was better than that of the control group, and there was no difference in the ALP content between the two groups. It is suggested that the influencing factors of ALP content in vivo may be not only hyperuricemia, but also the comprehensive effect of other complex changes in vivo. The specific reasons need to be further studied.
【學位授予單位】：北京中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R245

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