本文選題：針刺 + 哮喘��； 參考：《遼寧中醫(yī)雜志》2017年10期

【摘要】：目的:探討針刺對(duì)哮喘大鼠肺組織中P-AKT蛋白表達(dá)的影響。方法:將40只SPF級(jí)雄性SD大鼠隨機(jī)分為空白組、哮喘模型組、哮喘模型針刺組和哮喘模型霧化阻斷劑組,每組10只。各組分別給予相應(yīng)干預(yù)后,用HE染色觀察大鼠肺組織形態(tài)學(xué)改變,Western Blot法和免疫組織化學(xué)法檢測(cè)大鼠肺組織P-AKT蛋白的表達(dá)。結(jié)果:HE染色顯示哮喘模型組大鼠氣道周圍有多種炎癥細(xì)胞的浸潤和聚集,支氣管平滑肌痙攣,官腔狹窄;針刺組和霧化阻斷劑組有少量炎癥細(xì)胞,官腔狹窄管壁增厚程度較模型組減輕。Western Blot和免疫組化法檢測(cè)示哮喘模型組P-AKT蛋白表達(dá)較空白組、針刺組、霧化阻斷劑組升高(P0.05);針刺后P-AKT蛋白表達(dá)降低(P0.05),霧化阻斷劑組與空白組無差異(P0.05)。結(jié)論:針刺可降低大鼠肺組織中P-AKT蛋白表達(dá)以減輕炎癥反應(yīng)和氣道重塑。
[Abstract]:Objective: to investigate the effect of acupuncture on the expression of P-AKT protein in lung tissue of asthmatic rats. Methods: forty SPF male Sprague-Dawley rats were randomly divided into blank group, asthma model group, asthma model acupuncture group and asthma model atomization blocker group with 10 rats in each group. The morphological changes of lung tissue were observed by HE staining and the expression of P-AKT protein in rat lung tissue was detected by immunohistochemical method and Western Blot method. Results in the asthmatic model group, there were many inflammatory cells infiltrating and aggregating around the airway, bronchial smooth muscle spasm and stenosis of the functional cavity, and a small number of inflammatory cells in the acupuncture group and atomization blocker group. Compared with the model group, the thickening degree of the stenosis wall of the functional cavity decreased. Western Blot and immunohistochemical method showed that the expression of P-AKT protein in the asthmatic model group was higher than that in the blank group and the acupuncture group. The expression of P-AKT protein decreased after acupuncture, but there was no difference between the atomization blocker group and the blank group. Conclusion: acupuncture can reduce the expression of P-AKT protein in rat lung tissue and alleviate inflammation and airway remodeling.
【作者單位】： 華北理工大學(xué)中醫(yī)學(xué)院;
【分類號(hào)】：R245

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