本文選題：特發(fā)性肺纖維化 + 丹貝益肺方��； 參考：《黑龍江省中醫(yī)藥科學(xué)院》2016年碩士論文

【摘要】：目的:通過(guò)觀察丹貝益肺方作用于肺纖維化大鼠后,其肺組織中MMP-9和Egr-1表達(dá)的變化,及大鼠肺纖維化的改善情況,以驗(yàn)證丹貝益肺方的療效,并探討其作用機(jī)理。方法:取90只健康大鼠(Wistar),在清潔級(jí)實(shí)驗(yàn)室正常飼養(yǎng)一周。隨機(jī)平均分成5組,每組雌雄等量。其中1組用生理鹽水造模,標(biāo)記為空白組。其余4組均用博萊霉素造模,分別標(biāo)記為:模型組,潑尼松(陽(yáng)性對(duì)照)組,中藥(丹貝益肺)高、低劑量組。造模成功后,分別給與0.9%生理鹽水,潑尼松水溶液(濃度0.5mg/ml),丹貝益肺高劑量水煎液(濃度1.2g/ml),丹貝益肺低劑量水煎液(濃度0.3g/ml),均按體重比例(10ml/kg)灌胃。實(shí)驗(yàn)期間觀察大鼠一般狀態(tài),并在第7天、14天、28天時(shí),各組隨機(jī)處死6只大鼠并取材,測(cè)定大鼠肺系數(shù),觀察肺病理組織形態(tài)學(xué)變化,同時(shí)用相關(guān)分級(jí)評(píng)分標(biāo)準(zhǔn)對(duì)肺組織肺泡炎及纖維化程度進(jìn)行評(píng)分,并用免疫組織化學(xué)法檢測(cè)大鼠肺組織中MMP-9和Egr-1的表達(dá)水平。結(jié)果:中藥各劑量組及潑尼松組大鼠肺系數(shù)與模型組同期組間比較,均降低(P0.05),對(duì)照相應(yīng)病理組織切片觀察結(jié)果,提示潑尼松、丹貝益肺方高低計(jì)量均可減輕大鼠肺泡炎程度、減緩纖維化進(jìn)展。潑尼松組、中藥各劑量組大鼠的肺泡炎積分及纖維化積分低于同期模型組(P0.05),進(jìn)一步驗(yàn)證潑尼松和中藥高低劑量對(duì)大鼠肺泡炎及肺纖維化均有治療作用。與同期空白組相比,其余各組大鼠肺組織中MMP-9和Egr-1表達(dá)均增高(P0.05),提示肺纖維化的形成與MMP-9及Egr-1有關(guān);同期潑尼松組、中藥各劑量組肺組織中MMP-9和Egr-1表達(dá)均低于模型組,具有統(tǒng)計(jì)學(xué)差異性(P0.05),說(shuō)明丹貝益肺方對(duì)肺纖維化的治療機(jī)制可能與降低MMP-9及Egr-1的表達(dá)具有相關(guān)性。同期大鼠一般狀態(tài),潑尼松組及中藥各劑量組均優(yōu)于模型組,說(shuō)明潑尼松及丹貝益肺方可使肺纖維化大鼠的癥狀得到改善。結(jié)論:丹貝益肺方可改善肺纖維化大鼠癥狀表現(xiàn),降低大鼠肺系數(shù),減輕其肺泡炎及肺纖維化程度,減低肺組織中MMP-9和Egr-1的表達(dá),且該表達(dá)的減少可能是丹貝益肺對(duì)肺纖維化的治療機(jī)制之一。
[Abstract]:Aim: to observe the changes of MMP-9 and Egr-1 expression in lung tissue and the improvement of pulmonary fibrosis in rats after the action of Danbei Yifei recipe on pulmonary fibrosis in order to verify the curative effect of Danbei Yifei recipe and to explore its mechanism. Methods: 90 healthy rats were collected and fed in clean laboratory for one week. They were randomly divided into 5 groups with equal amount of both sexes in each group. The model was made with normal saline in group 1 and marked as blank group. The other four groups were made with bleomycin, which were labeled as model group, prednisone (positive control) group, high and low dose group of traditional Chinese medicine (Danbei Yifei). After the establishment of the model, 0.9% normal saline was given, prednisone solution (0.5 mg / ml), high dose Danbei Yifei decoction (1.2 g / ml) and low dose (0.3 g / ml 路ml ~ (-1) Danbei Yifei decoction (0.3 g / ml ~ (-1) 路kg ~ (-1) respectively. During the experiment, the general state of the rats was observed, and on the 7th day, the 14th day and the 28th day, 6 rats were killed randomly in each group, and the lung coefficient was measured, and the pathological changes of the lung were observed. At the same time, the degree of pulmonary alveolitis and fibrosis was evaluated by the correlation grading standard, and the expression of MMP-9 and Egr-1 in the lung tissue of rats was detected by immunohistochemical method. Results: compared with the model group, the lung coefficient of each dosage group and prednisone group decreased P0.05, and compared with the corresponding pathological tissue section observation, it suggested that prednisone and Danbei Yifei prescription could reduce the degree of alveolitis in rats. Slow the progression of fibrosis. The alveolitis score and fibrosis score of rats in prednisone group and traditional Chinese medicine group were lower than those in the model group (P 0.05). It was further proved that prednisone and high and low doses of traditional Chinese medicine had therapeutic effects on alveolitis and pulmonary fibrosis in rats. Compared with the blank group, the expression of MMP-9 and Egr-1 in the lung tissues of the other groups were all higher than that of the control group, indicating that the formation of pulmonary fibrosis was related to MMP-9 and Egr-1, and the expression of MMP-9 and Egr-1 in the lung tissues of the prednisone group and the traditional Chinese medicine group were all lower than those of the model group. There was statistical difference (P 0.05), which indicated that the therapeutic mechanism of Danbei Yifei recipe on pulmonary fibrosis might be related to the decrease of MMP-9 and Egr-1 expression. At the same time, the general state of rats, prednisone group and traditional Chinese medicine groups were better than the model group, indicating that prednisone and Danbei Yifei Fang could improve the symptoms of pulmonary fibrosis rats. Conclusion: danbei Yifei decoction can improve the symptoms of rats with pulmonary fibrosis, reduce the lung coefficient, reduce the degree of alveolitis and pulmonary fibrosis, and reduce the expression of MMP-9 and Egr-1 in lung tissue. The decrease of this expression may be one of the therapeutic mechanisms of lung fibrosis.
【學(xué)位授予單位】：黑龍江省中醫(yī)藥科學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R259
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本文編號(hào)：1835009