本文選題：骨關(guān)節(jié)炎 + 板橋黨參多糖��； 參考：《湖北民族學(xué)院》2016年碩士論文

【摘要】：目的:體外培養(yǎng)軟骨細(xì)胞系A(chǔ)TDC-5細(xì)胞,觀察板橋黨參多糖的干預(yù)對ATDC-5細(xì)胞活性的影響及NO的釋放量、i NOs的活力、IL-1β、II型膠原的表達(dá)影響,為豐富益氣法治療OA提供理論依據(jù)。方法:采用水提醇沉法提取板橋黨參多糖;體外培養(yǎng)ATDC-5細(xì)胞,CCK-8法檢測板橋黨參多糖對ATDC-5細(xì)胞活性的影響;將ATDC-5細(xì)胞分為5組,分別為空白組、LPS組、板橋黨參多糖組(低濃度組、中濃度組、高濃度組)。硝酸還原酶法檢測NO的釋放量、ELISA法檢測IL-1β的表達(dá)情況、Western Blot法檢測II型膠原的表達(dá)情況。結(jié)果:1提取板橋黨參多糖4.53g;2不同濃度板橋黨參多糖作用于ATDC-5細(xì)胞,1000μg/ml的多糖溶液能在一定程度抑制ATDC-5細(xì)胞的增殖活性,與空白組相比較P0.05,差異有統(tǒng)計學(xué)意義;3在LPS的作用下,ATDC-5細(xì)胞i NOs的活力、NO的釋放量、IL-1β的量均有所增加,與空白組相比較P0.05,差異有統(tǒng)計學(xué)意義;而加入板橋黨參多糖后,i NOs的活力、NO的釋放量、IL-1β的量明顯減少;4在LPS作用下,ATDC-5細(xì)胞表達(dá)的II型膠原會明顯減少與空白組相比較P0.05,差異有統(tǒng)計學(xué)意義,而板橋黨參多糖能對抗LPS抑制ATDC-5細(xì)胞合成II型膠原的作用。結(jié)論:1 LPS能刺激ATDC-5細(xì)胞產(chǎn)生大量的NO;2板橋黨參多糖能一定程度抑制LPS誘導(dǎo)ATDC-5細(xì)胞NO的釋放(通過抑制i NOs活性);3板橋黨參多糖能一定程度抑制LPS誘導(dǎo)的ATDC-5細(xì)胞IL-1β的表達(dá);4板橋黨參多糖能對抗LPS抑制ATDC-5細(xì)胞合成II型膠原的作用,使ATDC-5細(xì)胞在受到炎性刺激的時候還能表達(dá)相對正常的II型膠原。
[Abstract]:Objective: to culture chondrocyte ATDC-5 cells in vitro, observe the effect of Codonopsis pilosula polysaccharide on the activity of ATDC-5 cells and the expression of IL-1 尾 type II collagen in the release of no and the activity of I NOs, and provide a theoretical basis for enriching the method of supplementing qi to treat OA. Methods: the polysaccharides of Codonopsis pilosula were extracted by water extraction and alcohol precipitation, the effect of polysaccharides of Codonopsis pilosula on the activity of ATDC-5 cells was detected by CCK-8 method in vitro cultured ATDC-5 cells, and the ATDC-5 cells were divided into five groups: blank group, low concentration group. Medium concentration group, high concentration group. No release was detected by nitrate reductase method and the expression of IL-1 尾 by Elisa and type II collagen by Western Blot. Results the polysaccharides of Panqiao Codonopsis pilosula with different concentrations (4.53g / g) could inhibit the proliferative activity of ATDC-5 cells in 1 000 渭 g/ml solution of 1 000 渭 g/ml in ATDC-5 cells. Compared with the control group (P0.05), the difference was statistically significant (P 0.05). The activity of NOs in ATDC-5 cells induced by LPS was increased and the amount of IL-1 尾 was increased. The difference was statistically significant compared with the control group (P0.05). However, the activity of NOs and the amount of IL-1 尾 after adding Panqiao Codonopsis pilosula polysaccharides decreased significantly the expression of type II collagen in ATDC-5 cells induced by LPS was significantly decreased compared with the control group (P 0.05), and the difference was statistically significant. The polysaccharide of Codonopsis pilosula could inhibit the synthesis of type II collagen by LPS. Conclusion 1 LPS can stimulate the production of a large amount of No2 Codonopsis pilosula polysaccharide from ATDC-5 cells. It can inhibit the release of no from ATDC-5 cells induced by LPS to some extent (by inhibiting the activity of I NOs), Codonopsis lanceolata polysaccharide can inhibit IL-1 尾 of ATDC-5 cells induced by LPS to some extent. Dangshen polysaccharide could antagonize the effect of LPS on the synthesis of type II collagen in ATDC-5 cells. ATDC-5 cells were also able to express type II collagen in response to inflammatory stimulation.
【學(xué)位授予單位】：湖北民族學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R274.9

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