本文選題：出血性絡(luò)風(fēng)內(nèi)動證 + 動物模型��； 參考：《中醫(yī)雜志》2017年11期

【摘要】：目的建立符合臨床、具有易行性與廣泛適用性的出血性絡(luò)風(fēng)內(nèi)動證動物模型。方法 Apo E(-/-)基因敲除小鼠高脂飼料喂養(yǎng)并于12周齡行右側(cè)頸總動脈套管術(shù),術(shù)后隨機分為對照組、應(yīng)激組、Compound組、應(yīng)激+Compound組。術(shù)后4周后對所有小鼠腹腔注射脂多糖1mg/kg,每周2次,共8周;術(shù)后8周應(yīng)激組、應(yīng)激+Compound組給予限制性精神刺激,每天4h,共4周;術(shù)后10周Compound組、應(yīng)激+Compound組小鼠腹腔注射Compound 48/80 0.5 mg/kg,隔日1次,共4次。術(shù)后12周進行血脂檢測和斑塊病理形態(tài)學(xué)檢測,計算斑塊面積、血管管腔狹窄率,比較斑塊內(nèi)脂質(zhì)、膠原含量、平滑肌細胞和單核/巨噬細胞含量并計算斑塊易損指數(shù)。結(jié)果各組小鼠血脂水平差異均無統(tǒng)計學(xué)意義(P0.05)。與對照組比較,Coumpound組斑塊內(nèi)出血情況明顯升高,應(yīng)激+Compound組總斑塊病理形態(tài)情況差異有統(tǒng)計學(xué)意義(P0.05)。各組斑塊面積、血管管腔狹窄率及斑塊中脂質(zhì)、平滑肌細胞含量比較差異均無統(tǒng)計學(xué)意義(P0.05),而應(yīng)激+Compound組和Coumpound組的單核/巨噬細胞含量則較對照組明顯升高(P0.05)。應(yīng)激+Compound組斑塊內(nèi)膠原含量較其余各組減少,而易損指數(shù)較其余各組升高(P0.05)。結(jié)論采用脂多糖+應(yīng)激+Compound 48/80三種因素聯(lián)合刺激進行出血性絡(luò)風(fēng)內(nèi)動證小鼠模型構(gòu)建具有較高的斑塊易損性和斑塊內(nèi)出血率,可作為出血性絡(luò)風(fēng)內(nèi)動證動物模型的最佳造模方法。
[Abstract]:Objective to establish an animal model of hemorrhagic collateral wind syndrome with clinical, easy and wide applicability. Methods Apo E- / R) knockout mice were fed with high fat diet and were treated with right common carotid artery cannula at the age of 12 weeks. After the operation, they were randomly divided into control group, stress group and stress Compound group. Four weeks after operation, all mice were intraperitoneally injected with lipopolysaccharide 1 mg / kg, twice a week for 8 weeks; in the stress group, the stress Compound group was given restricted mental stimulation for 4 weeks, 4 hours per day, and 10 weeks after the operation, in the Compound group, Mice in stress Compound group were injected Compound 48 / 80 0.5 mg / kg intraperitoneally once every other day for 4 times. The plaque area, vascular stenosis rate, lipid content, collagen content, smooth muscle cell and mononuclear / macrophage content were compared and plaque vulnerability index was calculated 12 weeks after operation. Results there was no significant difference in blood lipid level in each group (P 0.05). Compared with the control group, the plaque hemorrhage in the Coumpound group was significantly increased, and the pathological morphology of the total plaque in the stress Compound group was significantly higher than that in the control group (P 0.05). There was no significant difference in plaque area, vascular lumen stenosis rate, lipid content and smooth muscle cell content in each group, but the monocyte / macrophage content in stress Compound group and Coumpound group was significantly higher than that in control group. The content of collagen in plaque in stress Compound group was lower than that in other groups, while the vulnerability index was higher than that in other groups (P 0.05). Conclusion the model of hemorrhagic collateral wind induced by lipopolysaccharide stress (Compound 48 / 80) combined with stimulation has high plaque vulnerability and intraplaque bleeding rate. It can be used as the best modeling method for the animal model of hemorrhagic collateral wind internal movement syndrome.
【作者單位】： 北京中醫(yī)藥大學(xué)東直門醫(yī)院;
【基金】：國家自然科學(xué)基金(81273913) 北京中醫(yī)藥大學(xué)重點學(xué)科開放課題(2013-ZDXKKF-27)
【分類號】：R259;R-332

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