本文選題：環(huán)磷酰胺 + 骨髓抑制��； 參考：《河南中醫(yī)藥大學》2016年碩士論文

【摘要】：目的通過觀察針灸對環(huán)磷酰胺(Cyclophosphamide,CTX)所致骨髓抑制模型小鼠骨髓組織中基質(zhì)細胞衍生因子-l(SDF-1)及淋巴細胞相關(guān)抗原-1(LFA-1)含量的影響,從分子生物學水平揭示針灸修復和保護化療后骨髓造血微環(huán)境的作用機理,從而為臨床應(yīng)用針灸保護化療患者的造血功能,提升外周血白細胞提供分子生物學的實驗室依據(jù)。方法將80只SPF級雄性昆明種小鼠按治療天數(shù)不同依其體重隨機分為3天組和5天組,每組再按其體重隨機分成4組:空白組、模型組、針刺組、艾灸組;每亞組各10只。用CTX建立骨髓抑制模型。造模成功后,每天定時專人對針刺組、艾灸組小鼠“大椎”、“膈俞”、“腎俞”、“足三里”給予針刺、艾灸治療,根據(jù)所分組不同,分別連續(xù)治療3或5天�？瞻捉M、模型組每日陪同抓取固定,不治療。各組小鼠每日定時專人尾靜脈采血,鏡檢外周血白細胞計數(shù);酶聯(lián)免疫吸附試驗檢測各組小鼠骨髓細胞SDF-1和LFA-1含量,采用SPSS20.0 for Windows統(tǒng)計軟件進行數(shù)據(jù)統(tǒng)計分析。結(jié)果1造模后,3天組和5天組接受CTX化療的各組小鼠與空白組比白細胞數(shù)明顯下降(P0.05),提示2組均造模成功。治療3天后,模型組、針刺組、艾灸組白細胞數(shù)均低于空白組(P0.05)。治療5天后,模型組白細胞數(shù)低于空白組(P0.05),而針刺組、艾灸組白細胞數(shù)均高于模型組和空白組(P0.05)。模型組造模前白細胞最高,然后呈下降趨勢,在治療3天后白細胞降至最低,然后呈上升趨勢。針刺組、艾灸組造模前白細胞最高,然后呈下降趨勢,在治療第2天后白細胞最低,然后呈上升趨勢。2造模后,接受CTX化療的各組小鼠SDF-1含量與空白組比明顯下降(P0.05),提示CTX抑制骨髓趨化因子SDF-1的表達。治療3天與5天后SDF-1含量變化規(guī)律非常相近。與空白組比,模型組、針刺組、艾灸組SDF-1含量有所下降(P0.05)。但針刺組、艾灸組SDF-1的含量明顯高于模型組(P0.05),針刺組與艾灸組比,艾灸組略高于針刺組但差異無統(tǒng)計學意義(P0.05)。3造模后,接受CTX化療的各組小鼠LFA-1含量與空白組比明顯升高(P0.05),提示CTX促進骨髓黏附分子LFA-1的表達。治療3天后與治療5天后LFA-1含量變化規(guī)律大致相同:與空白組比,模型組、針刺組、艾灸組LFA-1含量有所上升(P0.05),但針刺組、艾灸組LFA-1含量明顯低于模型組(P0.05)。治療3天后與治療5天后兩者的差別在于:治療3天后針刺組與艾灸組比,艾灸組LFA-1略低于針刺組但差異無統(tǒng)計學意義(P0.05),治療5天后艾灸組LFA-1低于針刺組且差異有統(tǒng)計學意義(P0.05)。4治療天數(shù)不同對SDF-1、LFA-1含量的影響。針刺3天或5天對小鼠骨髓中SDF-1、LFA-1含量的影響,差異均無統(tǒng)計學意義(P0.05)。艾灸3天或5天對小鼠骨髓中SDF-1表達含量的差異亦無統(tǒng)計學意義(P0.05),但艾灸5天組小鼠骨髓中LFA-1含量低于艾灸3天組小鼠骨髓中LFA-1含量(P0.05)。結(jié)論1針灸治療可以拮抗CTX化療所致的骨髓抑制,改善骨髓造血功能,提高外周血白細胞計數(shù)。2針刺和艾灸可以通過上調(diào)CTX化療小鼠骨髓中趨化因子SDF-1的表達,增強造血干/祖細胞的趨化作用,調(diào)節(jié)骨髓中干/祖細胞的遷移,促進早期血細胞的生成和增殖,起到修復化療后骨髓造血微環(huán)境損傷的作用。3針刺和艾灸可以通過下調(diào)CTX化療小鼠骨髓中黏附因子LFA-1的表達,降低造血干細胞與骨髓基質(zhì)的牢固黏附,促進造血祖細胞增殖和分化,保護骨髓造血微環(huán)境,維護化療后骨髓造血功能。4針刺和艾灸相比,艾灸更有利于通過下調(diào)CTX化療小鼠骨髓中黏附因子LFA-1含量的途徑降低造血祖細胞與基質(zhì)的黏附,拮抗抑制性調(diào)節(jié)因子對造血祖細胞的損傷。
[Abstract]:Objective To observe the effect of acupuncture on the content of matrix cell derived factor -l (SDF-1) and lymphocyte associated antigen -1 (LFA-1) in bone marrow tissue of mice induced by Cyclophosphamide (CTX), and to reveal the mechanism of the action of acupuncture and moxibustion and protecting hematopoietic microenvironment after chemotherapy and chemotherapy. Clinical use of acupuncture and moxibustion to protect the hematopoietic function of chemotherapy patients and improve the molecular biology of peripheral blood leukocytes. Methods 80 SPF grade male Kunming mice were randomly divided into 3 days and 5 days according to their weight, and each group was divided into 4 groups according to their weight: blank group, model group, acupuncture group and moxibustion group. 10 rats in each subgroup were used to establish a bone marrow suppression model with CTX. After the success of the model, every day was given to the acupuncture group, the moxibustion group "Da Zhui", "Zushu", "Shenshu", "Zusanli", and moxibustion treatment. According to the different groups, the group was treated for 3 or 5 days. The blank group, the model group was accompanied daily and fixed, and the treatment was not treated. Group mice were given blood for collecting blood by tail vein every day, the white blood cell count of peripheral blood was examined by microscope, the content of SDF-1 and LFA-1 in bone marrow cells of each group were detected by enzyme linked immunosorbent assay, and the data were analyzed by SPSS20.0 for Windows software. The results of 1 models were compared with white blood cells in the 3 day group and 5 day group which received CTX chemotherapy. After 3 days, the number of white blood cells in model group, acupuncture group and moxibustion group were lower than that of blank group (P0.05). The number of white blood cells in model group was lower than that of blank group (P0.05) after 5 days treatment. The number of white blood cells in acupuncture group and moxibustion group were higher than that of model group and blank group (P0.05). The highest white blood cells in model group were before model building. After 3 days of treatment, the white blood cells decreased to the lowest, and then showed an upward trend. In the acupuncture group, the moxibustion group had the highest white blood cells before making the model, and then showed a downward trend. After second days of treatment, the white blood cells were the lowest, and then the.2 model was on the rise. The SDF-1 content in each group of rats received CTX chemotherapy was significantly lower than that in the blank group (P0.05), suggesting CTX Inhibition of the expression of bone marrow chemotactic factor SDF-1. The changes of SDF-1 content in 3 days and 5 days were very similar. Compared with the blank group, the content of SDF-1 in the model group, the acupuncture group and the moxibustion group decreased (P0.05). However, the content of SDF-1 in the acupuncture group and moxibustion group was significantly higher than that of the model group (P0.05), the ratio of the acupuncture group to the moxibustion group and the moxibustion group were slightly higher than the acupuncture group, but the difference was slightly higher than that in the acupuncture group. Without statistical significance (P0.05).3, the content of LFA-1 in each group of mice received CTX chemotherapy was significantly higher than that in the blank group (P0.05), suggesting that CTX promoted the expression of LFA-1 of bone marrow adhesion molecule. The changes of LFA-1 content in 3 days after treatment and 5 days after treatment were roughly the same as that in the blank group, and the LFA-1 content in the model group, the acupuncture group and the moxibustion group increased (P0.0). 5), but in the acupuncture group, the content of LFA-1 in the moxibustion group was significantly lower than that of the model group (P0.05). The difference between the 3 days after treatment and the treatment for 5 days was that the LFA-1 of the acupuncture group and the moxibustion group was slightly lower than the acupuncture group for 3 days, but the difference was not statistically significant (P0.05), and the LFA-1 in the moxibustion group was lower than the acupuncture group for 5 days, and the difference was statistically significant (P0.05).4. The effect of different treatment days on the content of SDF-1 and LFA-1. The effect of 3 days or 5 days on the content of SDF-1 and LFA-1 in the bone marrow of mice was not statistically significant (P0.05). The difference of SDF-1 expression in the bone marrow of mice was not statistically significant (P0.05) at 3 days or 5 days, but the content of LFA-1 in the bone marrow of the 5 day moxibustion group was lower than that of the 3 day moxibustion group. Conclusion the content of LFA-1 in rat bone marrow (P0.05). Conclusion 1 acupuncture and moxibustion therapy can antagonize the bone marrow suppression caused by CTX chemotherapy, improve the hematopoietic function of bone marrow, improve the peripheral blood leukocyte count,.2 acupuncture and moxibustion can increase the expression of chemokine SDF-1 in the bone marrow of the mice with CTX chemotherapy, enhance the chemotactic effect of the blood stem / progenitor cells and regulate the stem / progenitor in the bone marrow. Cell migration promotes the formation and proliferation of early blood cells, and plays the role of repairing hematopoietic microenvironment injury after chemotherapy.3 acupuncture and moxibustion can reduce the expression of adhesion factor LFA-1 in the bone marrow of mice with CTX chemotherapy, reduce the strong adhesion of hematopoietic stem cells and bone marrow stroma, promote the proliferation and differentiation of hematopoietic progenitor cells and protect the bone. Compared with.4 acupuncture and moxibustion, moxibustion is more beneficial to reduce the adhesion of the hematopoietic progenitor cells to the matrix by lowering the content of the adhesion factor LFA-1 in the bone marrow of the mice treated with CTX, and antagonize the damage of the inhibitory regulatory factors to the hematopoietic progenitor cells.

【學位授予單位】：河南中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R245

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