本文選題：電針治療 + 顳下頜關(guān)節(jié)紊亂綜合征��； 參考：《廣州中醫(yī)藥大學(xué)》2016年碩士論文

【摘要】：研究目的：顳下頜關(guān)節(jié)紊亂綜合癥(temporomandibular joint dysfunction syndrome,TMJDS),又稱顳下頜功能紊亂病(temporomandibular disorders, TMD),是口腔科的常見疾病,臨床癥狀較重,對(duì)患者的生活影響較大,會(huì)引起咀嚼功能和語言障礙；TMD的病程較長(zhǎng),反復(fù)難愈,給患者心理上蒙上一層厚厚的陰影,該疾病給患者帶來精神方面和生活方面的負(fù)面影響較大。深入探討顳下頜關(guān)節(jié)紊亂綜合癥的病因病機(jī)和發(fā)病機(jī)制,尋找有效的治療方法,是現(xiàn)代醫(yī)學(xué)研究的重要任務(wù)。本實(shí)驗(yàn)通過拔出大鼠右上頜磨牙,使大鼠長(zhǎng)期偏側(cè)咀嚼而造成顳下頜關(guān)節(jié)紊亂綜合癥實(shí)驗(yàn)動(dòng)物模型,以炎性因子IL-1β TNF-α為觀察指標(biāo),觀察電針治療對(duì)顳下頜關(guān)節(jié)紊亂綜合征大鼠的血清和顳下頜關(guān)節(jié)及組織內(nèi)炎性因子TNFα、IL-1β的影響。從細(xì)胞分子水平探討電針療法治療顳下頜關(guān)節(jié)紊亂綜合癥的作用機(jī)理,為電針療法治療顳下頜關(guān)節(jié)紊亂綜合癥給予科學(xué)的理論支持。研究方法：SPF級(jí)SD大鼠共30只,雌雄各半,體重180-220g,實(shí)驗(yàn)分電針治療組、空白對(duì)照組、模型組3組,每組10只。通過拔除大鼠右上頜磨牙,人為造成大鼠偏側(cè)咀嚼,建立大鼠顳下頜關(guān)節(jié)紊亂綜合征(TMD)模型。造模2周后進(jìn)行電針治療,針刺取穴為“下關(guān)”、“太陽”穴,導(dǎo)線連接雙側(cè)“太陽”和“下關(guān)”穴,治療時(shí)間為兩周,模型組和空白組大鼠每天抓握5分鐘,抓握方式同電針組。持續(xù)2周治療完成后,在不同組別的大鼠中采血并提取血清運(yùn)用酶聯(lián)免疫吸附反應(yīng)(ELISA)法檢測(cè)各組TNFα、IL-1β水平,處死大鼠后提取顳下頜關(guān)節(jié)及相關(guān)組織,研磨勻漿后取上清液運(yùn)用酶聯(lián)免疫吸附反應(yīng)(ELISA)法檢測(cè)各組TNFα、IL-1β水平,分析它們之間的關(guān)系,并運(yùn)用統(tǒng)計(jì)學(xué)方法對(duì)比各組實(shí)驗(yàn)數(shù)據(jù),采用SPSS19.0統(tǒng)計(jì)軟件進(jìn)行統(tǒng)計(jì)學(xué)處理。結(jié)果：模型組與空白組對(duì)照比較,模型組大鼠顳下頜關(guān)節(jié)內(nèi)IL-1β、TNF-α水平大幅升高(p0.05)；與模型組對(duì)照比較,各電針組IL-1β、TNF-α含量明顯降低(p0.05)。電針治療對(duì)大鼠顳下頜關(guān)節(jié)內(nèi)的炎性因子IL-1β、TNF-α的影響密切相關(guān),差異有統(tǒng)計(jì)學(xué)意義(p0.05)。結(jié)論：1.電針能夠有效降低大鼠血清中IL-1β、TNF-α等促炎因子的表達(dá)水平,從而提高血清抗炎因子的表達(dá)水平。2.電針治療可以通過調(diào)節(jié)與顳下頜關(guān)節(jié)紊亂綜合癥有關(guān)的炎癥反應(yīng),抑制相關(guān)炎癥反應(yīng),控制顳下頜關(guān)節(jié)紊亂綜合癥的發(fā)作,進(jìn)而抑制顳下頜關(guān)節(jié)紊亂綜合癥的病變過程。3.電針治療可明顯抑制模型大鼠顳下頜關(guān)節(jié)炎的指數(shù),具有一定抗炎消腫鎮(zhèn)痛作用,達(dá)到治療的目的。
[Abstract]:Objective: temporomandibular joint dysfunction syndrome is a common disease in stomatology. TMD can cause masticatory function and language disorder, the course of TMD is longer, and it is difficult to heal repeatedly, and the patient is covered with a thick shadow on the psychology. The disease has a great negative effect on the mental and life aspects of the patients. It is an important task of modern medical research to explore the etiology, pathogenesis and pathogenesis of temporomandibular joint disorder syndrome and to find effective treatment methods. The experimental animal model of temporomandibular joint disorder syndrome was established by pulling out the right maxillary molars in rats, and the inflammatory factor IL-1 尾 TNF- 偽 was used as the observation index. To observe the effect of electroacupuncture on the inflammatory factor TNF 偽 and IL-1 尾 in serum, temporomandibular joint (TMJ) and temporomandibular joint (TMJ) in rats with temporomandibular disorder syndrome (TMJ). To explore the mechanism of electroacupuncture in the treatment of temporomandibular joint disorder syndrome from the cellular and molecular level, and to provide scientific theoretical support for electroacupuncture treatment of temporomandibular joint disorder syndrome. Methods Thirty Sprague-Dawley rats of SPF grade were divided into three groups: electroacupuncture group, blank control group and model group with 10 rats in each group. The rat model of temporomandibular joint disorder syndrome (TMJS) was established by pulling out the right maxillary molars and making partial mastication. After 2 weeks of model making, the rats were treated with electroacupuncture. The acupuncture points were "Xiaguan", "Sun" and "Sun" points. The treatment time was two weeks. The rats in the model group and blank group were clasped for 5 minutes every day. Grip mode is the same as electroacupuncture group. After 2 weeks of treatment, blood was collected from different groups of rats and serum was extracted to detect the level of TNF 偽 and IL-1 尾 by Elisa. The temporomandibular joint and related tissues were extracted after the rats were killed. After grinding homogenate, the supernatant was collected to detect the level of TNF 偽 and IL-1 尾 in each group by enzyme linked immunosorbent assay (Elisa), and the relationship between them was analyzed. The statistical method was used to compare the experimental data of each group and the statistical software SPSS19.0 was used to deal with them. Results: compared with the control group, the level of IL-1 尾 -TNF- 偽 in the temporomandibular joint in the model group was significantly higher than that in the control group, and the content of IL-1 尾 -TNF- 偽 in each electroacupuncture group was significantly lower than that in the model group. The effect of electroacupuncture on the inflammatory factor IL-1 尾 -TNF- 偽 in temporomandibular joint of rats was closely related, and the difference was statistically significant (P 0.05). Conclusion 1. Electroacupuncture can effectively reduce the expression of proinflammatory factors such as IL-1 尾 -TNF- 偽 in serum of rats, and thus increase the expression level of anti-inflammatory factors in serum. Electroacupuncture can control the attack of temporomandibular joint disorder syndrome by regulating the inflammatory response associated with temporomandibular joint disorder syndrome, and then inhibit the pathological process of temporomandibular joint disorder syndrome. Electroacupuncture can obviously inhibit the index of temporomandibular arthritis in model rats.
【學(xué)位授予單位】：廣州中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R245

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