本文選題：創(chuàng)傷后應激障礙 + 胎損��； 參考：《成都中醫(yī)藥大學》2016年碩士論文

【摘要】：[目的]1.通過觀測創(chuàng)傷后應激心理障礙孕鼠“胎損”子代出生情況、生長發(fā)育、行為表現(xiàn)、血清神經(jīng)遞質,研究“胎損”對SD孕鼠子代的行為遺傳及神經(jīng)生物學損害；2.正確評估補腎中藥金匱腎氣丸聯(lián)合優(yōu)裕環(huán)境情志調護措施對創(chuàng)傷后應激心理障礙孕鼠“胎損”致子代生長發(fā)育遲緩、行為表現(xiàn)異常及血清神經(jīng)遞質紊亂的干預效果,科學闡釋“母病及子”、“恐傷腎”、“腎主生殖”、“腎主骨生髓”等經(jīng)典中醫(yī)理論內涵。[方法]將初次受孕的SD孕鼠(36只)采用隨機數(shù)字表分為三組：對照組、PTSD組以及情志+中藥組(以下簡稱情中組),每組12只。PTSD組大鼠和情中組大鼠于孕中期(第7-13d)采用單程長時應激法進行創(chuàng)傷應激造模。妊娠14天至分娩,PTSD組和對照組孕鼠正常實驗條件下普通飼養(yǎng)籠一般飼料常規(guī)喂養(yǎng),情中組孕鼠給予補腎中藥金匱腎氣丸及優(yōu)裕環(huán)境情志調護措施。記錄各組孕鼠妊娠并觀察有無流產及早產狀況；重復測量各組子鼠體重、身長、尾長(出生第0、5、15、25d)、張耳(出生第5d)等生長發(fā)育指標；在不同時間段對各組子鼠進行行為能力測試,包括翻身測試(出生60s內)、亮盒排便測試(子鼠出生第20d、30d)、糖水消耗試驗(子鼠出生第26d)、高架十字迷宮實驗(子鼠出生第30d)。股動脈采血(子鼠出生第30d),采用酶聯(lián)免疫吸附法對子代鼠的皮質酮、5-羥色胺、多巴胺等血清神經(jīng)遞質水平進行檢測。[結果]1.生長發(fā)育指標各組孕鼠都正常分娩,均無早產及流產情況。PTSD組出生體重、身長、尾長均低于對照組和情中組,差異有統(tǒng)計學意義(P0.05)；情中組和對照組出生體重比較差異有統(tǒng)計學意義(P0.05),身長、尾長比較差異無統(tǒng)計學意義(P0.05)對子鼠第5、15、25天體重、身長、尾長動態(tài)連續(xù)測量,各組之間體重、身長、尾長差異均有統(tǒng)計學意義(P組間0.05)。體重、身長、尾長受時間因素影響,不同時間點上體重、身長、尾長比較,差異有統(tǒng)計學意義(P時間0.05)；時間因素和分組因素的交互作用具有統(tǒng)計學意義(P交互0.05),即體重、身長、尾長隨時間變化的趨勢因分組不同而不同。通過各時間點各個指標比較得出：PTSD組生長發(fā)育最慢,而情中組生長發(fā)育較快且優(yōu)于對照組。PTSD組和對照組張耳情況比較,差異無統(tǒng)計學意義(P0.05)；情中組張耳發(fā)育優(yōu)于對照組和PTSD組,差異有統(tǒng)計學意義(P0.05)2.行為能力測試翻身測試中,PTSD組翻身能力低于對照組和情中組,差異有統(tǒng)計學意義(P0.01)；情中組和對照組翻身能力比較,差異無統(tǒng)計學意義(P0.05)。亮盒排便測試中,PTSD組大小便粒／次均高于其余兩組,差異有統(tǒng)計學意義(P0.05)；情中組與對照組比較,大便粒數(shù)差異有統(tǒng)計學意義(P0.05),小便次數(shù)差異無統(tǒng)計學意義(P0.05)。重復測量時間比較,時間差異有統(tǒng)計學意義(P0.05)。糖水消耗試驗中,PTSD組糖水偏嗜度低于情中組,差異有統(tǒng)計學意義(P0.01)；情中組和對照組糖水偏嗜度比較,差異無統(tǒng)計學意義(P0.05)高架十字迷宮實驗中,PTSD組開臂進入次數(shù)百分比和開臂停留時間百分比低于情中組和對照組,差異有統(tǒng)計學意義(P0.05)；情中組開臂進入次數(shù)百分比和開臂停留時間百分比均高于對照組,差異有統(tǒng)計學意義(P0.05)。3.血清神經(jīng)遞質PTSD組血清皮質酮水平高于情中組和對照組,差異有統(tǒng)計學意義(P0.05)；情中組和對照組皮質酮比較,差異無統(tǒng)計學意義(P0.05)。PTSD組血清5-羥色胺水平低于情中組和對照組,差異有統(tǒng)計學意義(P0.05)；情中組5-羥色胺水平高于對照組,差異有統(tǒng)計學意義(P0.05)。PTSD組血清多巴胺水平低于對照組,差異有統(tǒng)計學意義(P0.05)；情中組和對照組多巴胺水平比較,差異無統(tǒng)計學意義(P0.05)。[結論]1.母鼠孕期遭受創(chuàng)傷恐懼應激的危害會遺傳到子代,導致子代低出生體重(身長尾長)、生長發(fā)育遲緩、行為表現(xiàn)及血清神經(jīng)遞質異常等。2.中醫(yī)調護措施可調節(jié)并糾正PTSD'‘胎損”致子代生長發(fā)育遲緩、行為能力下降及神經(jīng)內分泌紊亂等。3.“恐傷腎”是PTSD“胎損”的病機之一。對“胎損”子代神經(jīng)生物學調節(jié)可通過先天補腎精、溫腎陽及后天疏肝理氣,達到氣血生化充足實現(xiàn)。
[Abstract]:Objective : To study the genetic and neurobiological damage of the offspring of SD pregnant rats by observing the birth , development , behavior and neurotransmitters in the offspring of pregnant rats with stress after trauma .
2 . To evaluate the effect of the traditional Chinese medicine ( Jingui Shenqi Wan ) combined with excellent environment emotion regulation on the growth retardation , abnormal behavior and serum neurotransmitters in the pregnant rats with post - traumatic stress after trauma .
The growth and development indexes such as body weight , body length , tail length ( 0 , 5 , 15 , 25 d ) and ear ( day 5 ) of each group were measured repeatedly .
The levels of serum neurotransmitters such as cortical ketone , 5 - HT and dopamine were measured by enzyme - linked immunosorbent assay . The results showed that the body weight , body length and tail length were lower than those in the control group and the case group ( P0.05 ) .
Body weight , body length and tail length were statistically significant ( P < 0.05 ) . Body weight , body length , tail length were affected by time factors , body weight , body length and tail length were statistically significant ( P < 0.05 ) .
The time factor and the interaction of grouping factors had statistical significance ( P < 0.05 ) , that is , the trend of body weight , body length and tail length changed from time to time .
There was significant difference ( P 0.05 ) 2 . In the test of behavioral ability test , the ability to turn over was lower than that in the control group and the case group ( P0.01 ) .
Compared with the control group , the difference was not statistically significant ( P0.05 ) .
Compared with the control group , the difference of stool number was significant ( P0.05 ) . There was no statistical significance ( P0.05 ) . The time difference was statistically significant ( P0.05 ) .
Compared with the control group , there was no statistical significance ( P0.05 ) . In the elevated cross maze test , the percentage of open arms and the percentage of staying time of open arms were lower than those in the control group and the control group ( P0.05 ) .
The percentage of open arms and the percentage of staying time of open arms were higher than those in the control group ( P0.05 ) .
Compared with the control group , the difference was not significant ( P0.05 ) . The levels of serum 5 - HT were lower than those in the control group and the control group ( P0.05 ) .
The levels of 5 - HT in the patients were higher than those in the control group ( P0.05 ) .
The results showed that the harm of the stress caused by traumatic stress in the pregnant period of the female rats could be inherited to the progeny , which led to the low birth weight ( long tail length ) , the growth and growth retardation , the behavior and the abnormal serum neurotransmitter .

【學位授予單位】：成都中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R248

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