本文選題：電針 + 依托咪酯�。� 參考：《臨床麻醉學(xué)雜志》2017年06期

【摘要】：目的探討電針足三里穴對依托咪酯麻醉大鼠應(yīng)激反應(yīng)的影響。方法選擇雄性SD大鼠60只,體重280~310g,隨機分為六組:空白對照組(C組)、模型組(M組)、依托咪酯60mg/kg組(E1組)、依托咪酯30 mg/kg組(E2組)、依托咪酯60 mg/kg聯(lián)合電針組(EA1組)和依托咪酯30mg/kg復(fù)合電針組(EA2組),每組10只。C組大鼠不進行任何麻醉手術(shù)操作,其余五組均建立低位尾干切斷模型。建模后,E1組和EA1組大鼠腹腔注射依托咪酯60mg/kg,E2組和EA2組大鼠腹腔注射依托咪酯30mg/kg。EA1組和EA2組大鼠于麻醉狀態(tài)行雙側(cè)足三里穴治療30min(頻率2/100Hz,疏密波,電流強度1mA)。采用ELISA法檢測血清ACTH和Cor的濃度,Western blot法檢測下丘腦室旁核組織c-fos蛋白的含量。結(jié)果 M組ACTH、Cor濃度和c-fos蛋白含量明顯高于C組(P0.05);E1組、E2組、EA1組、EA2組ACTH、Cor濃度和c-fos蛋白含量明顯低于M組(P0.05);E2組、EA1組ACTH、Cor濃度和c-fos蛋白含量明顯高于E1組(P0.05);EA2組ACTH濃度和c-fos蛋白含量明顯低于E2組(P0.05)。結(jié)論電針大鼠足三里穴可雙向調(diào)節(jié)依托咪酯對急性應(yīng)激反應(yīng)的影響,其機制可能與其調(diào)節(jié)下丘腦c-fos蛋白水平有關(guān)。
[Abstract]:Objective to investigate the effect of electroacupuncture at Zusanli point on the stress response of etomidate anesthetized rats.Methods 60 male SD rats were selected.Body weight 280 ~ 310g, randomly divided into six groups: blank control group (C), model group (M), etomidate 60mg/kg group (E _ 1), etomidate 30 mg/kg group (E _ 2), etomidate group (60 mg/kg combined with EA _ (1)) and etomidate 30mg/kg combined electroacupuncture group (EA _ 2 group).Each group of 10 rats in group C. did not perform any anesthetic operation.The other five groups established the model of low tail trunk transection.After modeling, Etomidate 60 mg / kg E2 group and EA2 group were injected intraperitoneally with etomidate 60 mg / kg E _ 2 and EA2 group. The rats of 30mg/kg.EA1 group and EA2 group were treated with bilateral Zusanli acupoint for 30 min (frequency 2 / 100 Hz, density wave, current intensity 1 Ma / L) in anaesthesia state.The concentration of serum ACTH and Cor were detected by ELISA and the content of c-fos in paraventricular nucleus of hypothalamus was detected by Western blot.Results the concentration of Cor and the content of c-fos protein in group M were significantly higher than those in group C (P 0.05) and group E _ 2 (group E _ 2) and the content of c-fos protein were significantly lower than those in group M (P 0.05) and c-fos protein in E _ 2 group than those in E _ 1 group (P 0.05) and c-fos egg group E _ 2 group (P < 0.05). The content of ACTHH Cor and c-fos protein in E _ 2 group were significantly higher than those in E _ 1 group and E _ 2 group, and the content of c-fos protein in E _ 2 group was significantly higher than that in E _ 2 group and E _ 2 group.The white content was significantly lower than that in E2 group (P 0.05).Conclusion electroacupuncture at Zusanli point can regulate the effect of etomidate on acute stress response in both directions, and its mechanism may be related to the regulation of c-fos protein level in hypothalamus.
【作者單位】： 桂林醫(yī)學(xué)院附屬醫(yī)院麻醉科;桂林醫(yī)學(xué)院附屬醫(yī)院中醫(yī)科;
【基金】：2012年廣西高校學(xué)�？蒲许椖�(201204LX243) 2014年桂林市科學(xué)研究與技術(shù)開發(fā)計劃課題(20140310-2-3)
【分類號】：R245
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本文編號：1757962