本文選題：針刺 + p38MAPK�。� 參考：《山東中醫(yī)藥大學》2016年碩士論文

【摘要】：目的:制備大腦中動脈栓塞(MCAO)大鼠模型,通過針刺其內關穴,檢測p38MA PK,p-p38MAPK,GLT-1在腦組織中的表達情況,初步探討p38MAPK,p-p38MAPK,GLT-1對谷氨酸-谷氨酰胺循環(huán)的調控作用,為針刺治療缺血性腦中風的作用機制研究提供新的研究思路和實驗依據(jù)。方法:采用線栓法建立大腦中脈栓塞(MCAO)大鼠模型,將大鼠分為模型組、內關組和地機組,另取假手術組為實驗對照組,內關組、地機組大鼠分別連續(xù)針刺內關穴、地機穴7d,對各組大鼠進行神經(jīng)體征評分,評估神經(jīng)功能恢復情況。多聚甲醛灌注后取腦,通過觀察腦組織石蠟切片HE染色檢測神經(jīng)元梗死面積,免疫組織化學檢測p38MAPK、p-p38MAPK、GLT-1表達變化;腦組織勻漿,利用Western Blot檢測方法,觀察針刺對MCAO大鼠腦組織p38MAPK、p-p38MAPK、GLT-1表達的影響。結果:1.連續(xù)針刺內關穴7d可顯著改善MCAO大鼠神經(jīng)功能,能夠促進其運動功能的恢復,內關穴的效果明顯優(yōu)于地機穴。2.針刺內關穴顯著上調MCAO大鼠腦組織中GLT-1的表達,下調p-p38MAPK、p38MAPK的表達,內關穴在影響p-p38MAPK、p38MAPK、GLT-1表達方面明顯優(yōu)于地機穴。結論:針刺內關穴能夠促進GLT-1的表達,減少p-p38MAPK、p38MAPK的表達,從而能夠顯著降低缺血腦組織中過度堆積的谷氨酸濃度,減輕其神經(jīng)毒性作用,有利于缺血腦組織神經(jīng)功能的恢復。
[Abstract]:Objective: to establish a rat model of middle cerebral artery embolization (MCAO) and to detect the expression of p38 MAPK-1 GLT-1 in brain tissue by acupuncture at its Neiguan point, and to explore the regulatory effect of p38 MAPK- p-p38 MAPK- GLT-1 on glutamic acid-glutamine circulation.To provide new ideas and experimental basis for the study of the mechanism of acupuncture in the treatment of ischemic stroke.Methods: the MCAO rat model was established by thread embolization. The rats were divided into three groups: model group, Neiguan group and ground unit. The sham operation group was used as the experimental control group, the Neiguan group and the ground unit group were used as the experimental control group, the Neiguan group and the ground unit group respectively.After 7 days, the nerve signs were scored to assess the recovery of nerve function.The infarct size of neurons was detected by HE staining in paraffin sections of brain tissue, the expression of p38MAPKnp-p38MAPKngt GLT-1 was detected by immunohistochemistry, and the brain homogenate was detected by Western Blot method.To observe the effect of acupuncture on the expression of GLT-1 in brain tissue of MCAO rats.The result is 1: 1.Continuous acupuncture at Neiguan acupoint for 7 days could significantly improve the nerve function and promote the recovery of motor function in MCAO rats. The effect of Neiguan acupoint was better than that of Diji acupoint. 2.Acupuncture at Neiguan significantly upregulated the expression of GLT-1 and down-regulated the expression of p-p38 MAPK p38 MAPK in the brain of MCAO rats. Neiguan acupoint was superior to Diji acupoint in influencing the expression of p-p38 MAPK- p38 MAPK- GLT-1.Conclusion: acupuncture at Neiguan point can promote the expression of GLT-1 and decrease the expression of p-p38 MAPK- p38 MAPK, which can significantly reduce the excessive accumulation of glutamate in ischemic brain tissue, alleviate its neurotoxic effect, and be beneficial to the recovery of neural function in ischemic brain tissue.
【學位授予單位】：山東中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R245.3

【相似文獻】

相關碩士學位論文 前1條

1 張旭生;針刺對MCAO大鼠腦功能恢復及p38MAPK，p-p38MAPK，GLT-1表達影響的研究[D];山東中醫(yī)藥大學;2016年

，

本文編號：1744254