本文選題：阿爾茨海默癥　切入點：電針　出處：《中國針灸》2017年06期 　論文類型：期刊論文

【摘要】：目的:觀察不同頻率電針"百會"與"腎俞"穴對阿爾茨海默病(AD)模型大鼠學習記憶能力和海馬組織糖原合酶激酶-3β(GSK-3β)、生長相關(guān)蛋白-43(GAP-43)的影響,探討不同頻率電針防治AD的作用機制。方法:將112只Wistar雄性大鼠按隨機數(shù)字表法分為正常組、假手術(shù)組、模型組、針刺組、2 Hz電針組、30 Hz電針組、50 Hz電針組,每組16只。正常組實驗室常規(guī)飼養(yǎng),不進行任何處理;假手術(shù)組于雙側(cè)海馬齒狀回注射0.9%Na Cl溶液;其他組采用雙側(cè)海馬齒狀回注射β-淀粉樣蛋白1-42(Aβ1-42)制備AD模型。造模成功15 d后,模型組、假手術(shù)組不進行任何處理,2 Hz、30 Hz、50 Hz電針組大鼠選"百會""腎俞"穴進行相應頻率電針治療,每日1次,7次為一療程,療程間休息1 d,共治療2個療程。針刺組選穴與電針組相同,但僅針刺不接電針。治療結(jié)束后立即進行水迷宮檢測,記錄大鼠逃避潛伏期、首次跨越平臺時間、跨越平臺次數(shù),并分別采用免疫組化法和Western blot檢測大鼠海馬組織GSK-3β、GAP-43的表達。結(jié)果:(1)水迷宮測試示,與正常組比較,模型組大鼠逃避潛伏期、首次跨越平臺時間延長(均P0.01),跨越平臺次數(shù)明顯減少(P0.01)。針刺組、3組電針組與模型組比較,逃避潛伏期、首次跨越平臺的時間縮短(均P0.01),跨越平臺次數(shù)明顯增加(P0.01)。50 Hz電針組與針刺組、2 Hz、30 Hz電針組比較,逃避潛伏期縮短(P0.01,P0.05)、首次跨越平臺時間縮短(均P0.01),跨越平臺次數(shù)明顯增加(P0.01,P0.05)。(2)與正常組比較,模型組GSK-3β、GAP-43表達明顯增多(均P0.01)。與模型組比較,針刺組和3組電針組GSK-3β表達明顯減少(均P0.01),GAP-43表達明顯增多(均P0.01)。與針刺組、2 Hz、30 Hz電針組比較,50 Hz電針組GSK-3β表達明顯減少,GAP-43表達增加(均P0.01)。結(jié)論:電針可能通過下調(diào)GSK-3β、上調(diào)GAP-43表達,從而促進突觸損傷修復,改善AD大鼠的學習記憶能力。50 Hz電針治療效果優(yōu)于30 Hz、2 Hz電針組及針刺組。
[Abstract]:Objective: to observe the effects of electroacupuncture of Baihui and Shenshu on learning and memory ability and glycogen synthase kinase-3 尾 -GSK-3 尾 -43 GAP-43 in hippocampus of rats with Alzheimer's disease (AD). Methods: 112 male Wistar rats were randomly divided into normal group, sham operation group, model group and acupuncture group. 16 rats in each group were fed in the normal laboratory without any treatment, and 0.9 NaCl solution was injected into the dentate gyrus of both sides in the sham operation group. The AD model was established by injecting 尾 -amyloid protein 1-42A 尾 1-42 into the dentate gyrus of the other groups. After 15 days of successful modeling, the model group was treated with 尾 -amyloid protein 1-42A 尾 1-42. Rats in the sham operation group were treated with "Baihui" and "Shenshu" at the corresponding frequency, 7 times a day as a course of treatment. The acupuncture group selected points the same as the electroacupuncture group, but only the acupuncture group did not receive electroacupuncture. After the treatment, the water maze test was carried out immediately, the escape latency of rats was recorded, the first time of crossing the platform, the times of crossing the platform, the time of crossing the platform, the time of crossing the platform for the first time, and the number of times of crossing the platform. Immunohistochemical method and Western blot were used to detect the expression of GSK-3 尾 -GAP-43 in hippocampus of rats. Results compared with the normal group, the escape latency of the model group was higher than that of the normal group. The time of crossing the platform was prolonged for the first time (all P0.01A, the times of crossing the platform were significantly decreased P0.01.The escape latency of EA group and model group was compared with that of model group. The time of crossing the platform for the first time was shortened (all P 0.01), and the times of crossing the platform were significantly increased compared with the acupuncture group and the acupuncture group. Compared with the normal group, the expression of GSK-3 尾 -GAP-43 in the model group was significantly higher than that in the normal group (P 0.01). The expression of GSK-3 尾 in acupuncture group and electroacupuncture group was significantly decreased (P 0.01) and the expression of GAP-43 was significantly increased (all P 0.01). Compared with acupuncture group, the expression of GSK-3 尾 in 50 Hz electroacupuncture group decreased significantly (all P 0.01). Conclusion: electroacupuncture may increase the expression of GSK-3 尾 (P 0.01). Regulation of GSK-3 尾, up-regulation of GAP-43 expression, The results showed that the effect of electroacupuncture on learning and memory ability of AD rats was better than that of 30 Hz / 2 Hz electroacupuncture group and 30 Hz / 2 Hz electroacupuncture group, so as to promote the repair of synaptic injury and improve the learning and memory ability of AD rats.
【作者單位】： 湖北中醫(yī)藥大學;湖北中醫(yī)藥大學針灸骨傷學院;
【基金】：國家自然科學基金資助項目:81373741
【分類號】：R245

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