本文選題：自然衰老　切入點(diǎn)：卵巢去勢(shì)　出處：《中華中醫(yī)藥學(xué)刊》2017年03期 　論文類型：期刊論文

【摘要】：目的:比較自然衰老法與卵巢去勢(shì)法建立的ICR小鼠圍絕經(jīng)期模型,并對(duì)模型進(jìn)行評(píng)價(jià)。方法:通過(guò)觀察陰道脫落細(xì)胞,比較自然衰老組及卵巢去勢(shì)組小鼠的動(dòng)情周期情況;觀察小鼠懸尾活動(dòng),比較自然衰老組及卵巢去勢(shì)組小鼠的情緒狀態(tài);測(cè)定血清性激素,比較血清卵泡刺激素(FSH)、促黃體激素(LH)、雌二醇(E2)的含量;測(cè)定小鼠下丘腦神經(jīng)肽,比較下丘腦Kiss-1及其受體GPR54 mRNA的表達(dá)。結(jié)果:自然衰老組及卵巢去勢(shì)組小鼠動(dòng)情周期均紊亂,動(dòng)情頻率均減少;兩組小鼠懸尾活動(dòng)比均顯著提高;兩組小鼠血清的FSH、LH水平顯著上升,E2水平明顯降低;兩組小鼠的下丘腦Kiss-1及受體GPR54 mRNA的表達(dá)顯著升高。相比卵巢去勢(shì)小鼠,自然衰老小鼠動(dòng)情周期相對(duì)延長(zhǎng),動(dòng)情頻率明顯減少,血清的FSH、LH水平上升更顯著,E2水平降低更顯著,Ki SS-1/GPR54 mRNA的表達(dá)升高更明顯。結(jié)論:自然衰老法與卵巢去勢(shì)法均能建立小鼠圍絕經(jīng)期模型,而自然衰老法優(yōu)于卵巢去勢(shì)法。
[Abstract]:Objective: to compare the peri-menopausal model of ICR mice established by natural aging method and ovariectomized method, and to evaluate the model. Methods: by observing vaginal exfoliated cells, the estrous cycle of natural aging group and ovariectomized group was compared. To observe the activity of tail suspension in mice, to compare the emotional state of mice in natural aging group and ovariectomized group, to measure serum sex hormone, to compare the contents of serum follicle stimulating hormone FSHN, luteinizing hormone LHN, estradiol E _ 2, and to determine the neuropeptide of hypothalamus of mice. Results: the oestrous cycle of mice in natural aging group and ovariectomized group was disorder, and the frequency of estrus was decreased. The levels of Kiss-1 and GPR54 mRNA in hypothalamus of the two groups increased significantly. Compared with ovariectomized mice, the oestrous cycle and the frequency of estrus decreased significantly in the naturally aging mice. The serum level of FSH LH increased significantly and the level of E2 decreased significantly. The expression of Ki SS-1/GPR54 mRNA increased more obviously. Conclusion: both natural senescence and ovariectomization can establish the model of mouse peri-menopausal period, and the natural aging method is better than the ovariectomized method.
【作者單位】： 杭州市中醫(yī)院;湖州市中心醫(yī)院;杭州市蕭山區(qū)第一人民醫(yī)院;
【基金】：全國(guó)名老中醫(yī)藥專家傅萍傳承工作室建設(shè)項(xiàng)目([2014]20號(hào)) 浙江省科技廳項(xiàng)目(2013C33211) 浙江省中醫(yī)藥管理局項(xiàng)目(2013ZQ025) 浙江省衛(wèi)生廳項(xiàng)目(2013KYA168) 杭州市科技局項(xiàng)目(20140733Q31) 浙江省名老中醫(yī)專家傅萍傳承工作室研究項(xiàng)目(GZS2012023)
【分類號(hào)】：R271.116;R-332

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