本文選題：形神共養(yǎng)　切入點：豐富生存環(huán)境　出處：《中國康復(fù)醫(yī)學(xué)雜志》2017年04期 　論文類型：期刊論文

【摘要】：目的:探討"形神共養(yǎng)"的豐富生存環(huán)境對正常大鼠認知功能、突觸素(SYN)、微管相關(guān)蛋白-2(MAP-2)的表達及大腦m PFC區(qū)錐體細胞的影響。方法:健康雄性SD大鼠48只,隨機分為"形養(yǎng)"組(EE1),"神養(yǎng)"組(EE2),"形神共養(yǎng)"組(EE3)和標準養(yǎng)組(SE),每組12只,分別進行形養(yǎng)、神養(yǎng)、形神共養(yǎng)的豐富生存環(huán)境訓(xùn)練及標準養(yǎng)。訓(xùn)練結(jié)束后進行水迷宮實驗,水迷宮結(jié)束后用免疫組織化學(xué)方法檢測海馬區(qū)SYN、MAP-2的表達;用Golgi-Cox染色觀察大腦內(nèi)側(cè)前額葉區(qū)錐體細胞樹突的變化。結(jié)果:水迷宮測試的第5天,EE3組大鼠的潛伏期明顯短于SE組(P0.05);4組大鼠在目標象限停留的時間無明顯差異(P0.05)。SE組的SYN表達最低,而EE1組和EE3組之間的表達無明顯差異且均高于EE2組。SE組的MAP-2表達最低,而EE3組的表達高于EE1組和EE2組。m PFC區(qū)錐體細胞樹突交點總數(shù)4組組間比較差異無顯著性意義(P0.05)。棘密度EE3組最大,其次是EE1組,SE組與EE2組之間無區(qū)別且較EE1和EE3組低。結(jié)論:"形神共養(yǎng)"的豐富生存環(huán)境可提高正常大鼠的認知功能,并促進SYN及MAP-2免疫表達及錐體細胞樹突生長。
[Abstract]:Objective: to investigate the effects of the rich living environment of "form and spirit co-nourishment" on cognitive function, synaptophysin and microtubulosis-associated protein (-2mMAP-2) expression in normal rats and the pyramidal cells in the m PFC region of the brain. Methods: 48 healthy male Sprague-Dawley rats were included in this study. They were randomly divided into three groups: "form nourishing" group, "spirit nourishing" group, "form and spirit common nourishing" group, "form and spirit raising together" group, and standard group, 12 rats in each group were trained in rich living environment and standard culture. After training, water labyrinth experiment was carried out. After the water maze was finished, the expression of SYN-MAP-2 in hippocampal area was detected by immunohistochemical method. The changes of dendritic cells of pyramidal cells in the medial prefrontal lobe of the brain were observed by Golgi-Cox staining. Results: the latency of rats in the EE3 group on the 5th day of water maze test was significantly shorter than that of the rats in the target quadrant of the SE group P0.05 + 4. There was no significant difference in the retention time of the rats in the target quadrant. The expression of SYN was the lowest in the group, However, there was no significant difference between EE1 group and EE3 group and the lowest expression of MAP-2 was found in EE2 group and SE group. However, the expression of dendritic cells in EE3 group was higher than that in EE1 group and EE2 group. There was no significant difference in the total number of dendritic cells in pyramidal cell in EE2 group. There was no significant difference between four groups (P 0.05). The spike density was the highest in EE3 group. Secondly, there was no difference between SE group and EE2 group in EE1 group, which was lower than that in EE1 and EE3 group. Conclusion: the rich living environment of "form and spirit co-nourishing" can improve the cognitive function of normal rats, and promote the expression of SYN and MAP-2 and the dendritic growth of pyramidal cells.
【作者單位】： 武漢大學(xué)中南醫(yī)院康復(fù)醫(yī)學(xué)科;湖北省中西醫(yī)結(jié)合醫(yī)院康復(fù)醫(yī)學(xué)科;
【基金】：國家自然科學(xué)基金項目(81173315)
【分類號】：R212

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