本文選題：逼尿肌反射亢進(jìn)　切入點：隔鹽隔姜灸　出處：《中國針灸》2017年04期 　論文類型：期刊論文

【摘要】：目的:探討隔鹽隔姜灸"神闕"穴對逼尿肌反射亢進(jìn)的效應(yīng)及其作用機(jī)制。方法:成年雌性SD大鼠30只,其中20只大鼠采用T9完全脊髓橫斷方法建立逼尿肌反射亢進(jìn)模型后隨機(jī)分為模型組(10只)和艾灸組(10只),余10只作為假手術(shù)組行假脊髓橫斷手術(shù)。艾灸組大鼠出現(xiàn)尿失禁(約造模2周)后,給以隔鹽隔姜灸"神闕"穴治療,每次灸3壯,每日1次,連續(xù)治療7d。各組在干預(yù)結(jié)束后,進(jìn)行尿流動力學(xué)檢測,之后取膀胱逼尿肌,采用免疫蛋白印跡法檢測M2、M3受體蛋白表達(dá)情況。結(jié)果:與假手術(shù)組大鼠相比,模型組大鼠排尿間期明顯較短(P0.01),最大膀胱內(nèi)壓明顯較高(P0.01),膀胱逼尿肌M2受體蛋白表達(dá)顯著升高(P0.05),M3受體蛋白表達(dá)無明顯變化(P0.05);與模型組相比,艾灸組大鼠排尿間期明顯較長(P0.01),最大膀胱內(nèi)壓明顯降低(P0.01),膀胱逼尿肌M2受體蛋白表達(dá)顯著下降(P0.05),而M3受體蛋白表達(dá)無明顯變化(P0.05);與假手術(shù)組相比,艾灸組以上指標(biāo)差異均無統(tǒng)計學(xué)意義(均P0.05)。結(jié)論:隔鹽隔姜灸"神闕"穴能抑制脊髓橫斷大鼠的膀胱過度活動狀態(tài),其作用機(jī)制可能與減少膀胱逼尿肌M2受體蛋白的表達(dá)、抑制逼尿肌的過度收縮有關(guān)。
[Abstract]:Objective: to investigate the effect and mechanism of salt-separated ginger-separated moxibustion on detrusor hyperreactivity in 30 adult female SD rats. Twenty rats were randomly divided into model group (n = 10) and moxibustion group (n = 10). The remaining 10 rats were used as sham operation group for pseudo spinal cord transection. After incontinence (about 2 weeks), The ginger-separated moxibustion was treated with "Shenque" point, 3 strong moxibustion every time, once a day, continuously for 7 days. After the intervention, the urinary fluid dynamics was measured, and the bladder detrusor muscle was taken out. The expression of M2M3 receptor protein was detected by Western blot. Results: compared with sham-operated rats, the expression of M2M3 receptor protein was detected by Western blot. In the model group, the urinary interval was significantly shorter, the maximal intravesical pressure was significantly higher than that in the control group, and the expression of M2 receptor protein in the bladder detrusor muscle was significantly higher than that in the model group, and there was no significant change in the expression of P0.05M3 receptor protein in the bladder detrusor muscle compared with the model group. In the moxibustion group, the urination interval was significantly longer than that in the control group, the maximal intravesical pressure decreased significantly, and the expression of M2 receptor protein in the bladder detrusor muscle decreased significantly, while the expression of M3 receptor protein did not change significantly in the moxibustion group, compared with the sham-operated group, the expression of M2 receptor protein in the bladder detrusor muscle decreased significantly, and the expression of M3 receptor protein did not change in the moxibustion group. Conclusion: moxibustion with ginger-separated moxibustion on "Shenque" can inhibit the overactive state of bladder in rats with spinal cord transection, and its mechanism may be related to reducing the expression of M2 receptor protein in bladder detrusor muscle. Inhibition of detrusor overcontraction is associated with.
【作者單位】： 天津中醫(yī)藥大學(xué)研究生院;首都醫(yī)科大學(xué)附屬北京中醫(yī)醫(yī)院針灸中心;
【基金】：國家自然科學(xué)基金資助項目:8140150462 北京市自然科學(xué)基金資助項目:7154206 北京市醫(yī)院管理局青年人才培養(yǎng)“青苗”計劃項目:QML20150904
【分類號】：R245

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