本文選題：潰瘍性結(jié)腸炎　切入點：EGF　出處：《遼寧中醫(yī)藥大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:從結(jié)腸粘膜屏障修復(fù)角度探討七及腸寧灌腸液治療大鼠潰瘍性結(jié)腸炎的療效。材料與方法:本實驗采用2,4,6-三硝基苯磺酸和乙醇混合來創(chuàng)造試驗?zāi)Ｐ?配好的溶液對大鼠進(jìn)行灌腸,組建潰瘍性結(jié)腸炎的實驗?zāi)Ｐ�。通過記錄各組大鼠身體體重的變化、大鼠行為學(xué)狀態(tài)、以及大鼠的糞便情況,椎脫臼處死大鼠。取實驗?zāi)Ｐ痛笫蟮慕Y(jié)腸組織8cm,結(jié)腸組織勻漿,測定結(jié)腸中EGF、BFGF、CD3和CD8的含量。通過制作組織切片來觀察分析;進(jìn)而探索七及腸寧灌腸液對潰瘍性結(jié)腸炎大鼠腸粘膜屏障的修護(hù)作用。結(jié)果:1.通過實驗發(fā)現(xiàn)X組動物整體狀態(tài)好轉(zhuǎn),病變部位治愈恢復(fù)相對好,并且直接表現(xiàn)在大鼠的體重增加上。2.模型組CD3和CD8細(xì)胞群明顯降低,七及腸寧灌腸液高劑量組能顯著改善這種情況。3.實驗群病變模型創(chuàng)造成功后,模型組EGF和BFGF的升高比其他組明顯。結(jié)論:1.七及腸寧灌腸液可以改善潰瘍性結(jié)腸炎模型大鼠膿血便癥狀,對其腸粘膜屏障有一定修復(fù)作用。2.七及腸寧灌腸液治療潰瘍性結(jié)腸炎的機(jī)理可能是通過下調(diào)CD3和CD8的含量,以糾正異常的免疫功能,從而達(dá)到消除炎癥,修復(fù)組織的作用。3.三硝基苯磺酸加乙醇灌腸法造模比較理想,既符合潰瘍性結(jié)腸炎發(fā)生的免疫學(xué)機(jī)制,又符合腸道炎癥發(fā)作期的特點,適合于觀察藥物療效比較和作用機(jī)理探討研究之用。
[Abstract]:Objective: to investigate the therapeutic effect of Qihehe Changning enema on ulcerative colitis in rats from the point of view of repairing colonic mucosal barrier. Materials and methods: the experimental model was created by mixing 2o 4N 6-trinitrobenzene sulfonic acid with ethanol. The rat model of ulcerative colitis was established by enema with a mixed solution. The changes of body weight, the behavioral state of rats and the feces of rats were recorded by recording the changes of body weight, the behavior of rats, and the feces of rats in each group. The rats were killed by dislocation of vertebrae. The colonic tissue was taken from the experimental model rats at 8 cm and the colonic tissue homogenate was used to determine the contents of CD3 and CD8 in the colon. The contents of CD3 and CD8 were observed and analyzed by making tissue sections. To explore the repairing effect of Qi and Changning enema liquid on intestinal mucosal barrier in rats with ulcerative colitis. Results: 1. It was found that the whole state of the animals in group X was improved and the lesion site was cured and recovered relatively well. The results showed directly in the weight gain of rats. 2. The CD3 and CD8 cell populations in the model group were significantly decreased, and those in the high dose group of 7 and Changning enema solution could significantly improve this situation. 3. After the establishment of the experimental group pathological model, the experimental group lesion model was established successfully. Conclusion the increase of EGF and BFGF in the model group is significantly higher than that in the other groups. Conclusion: 1. 7 and Changning enema can improve the symptoms of pus and stool in rats with ulcerative colitis. The mechanism of repairing intestinal mucosal barrier. 2.7and Changning enema solution in the treatment of ulcerative colitis may be that the abnormal immune function can be corrected by down-regulating the contents of CD3 and CD8 in order to eliminate inflammation. The effect of repairing tissue .3.The method of enema with trinitrobenzene sulfonic acid and ethanol is ideal, which not only accords with the immunological mechanism of ulcerative colitis, but also accords with the characteristics of intestinal inflammatory attack. It is suitable for observing the comparison of drug curative effect and exploring the mechanism of action.
【學(xué)位授予單位】：遼寧中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R259
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本文編號：1584664