本文關(guān)鍵詞： 電針 體外受精-胚胎移植 VEGF VEGFR　出處：《南京中醫(yī)藥大學學報》2017年06期 　論文類型：期刊論文

【摘要】：目的觀察電針刺激對激素促排卵大鼠子宮組織中VEGF、VEGFR2表達的影響,并探討其作用機制。方法 10周齡SD雌性大鼠30只,隨機分為對照組、模型組及治療組,每組各10只。模型組和治療組腹腔注射孕馬血清促性腺激素(PMSG)20U,48h后注射同等劑量的人絨毛膜促性腺激素(hCG),與種鼠合籠,次晨分籠造模,治療組在與種鼠合籠前1周連續(xù)電針治療7d(刺激強度1mA,疏密波2/15Hz,持續(xù)刺激15min)。合籠后第3.5天處死實驗動物,留取子宮組織,觀察形態(tài)改變;采用免疫組化方法檢測VEGF、VEGFR2蛋白的表達;采用qPCR技術(shù)測定子宮組織中VEGF、VEGFR2mRNA的表達,采用2~(-△△Ct)方法計算。結(jié)果肉眼觀察對照組子宮形態(tài)規(guī)整,表面紅潤光滑;模型組子宮形態(tài)腫大扭曲,表面色澤暗紅充血;治療組子宮形態(tài)略微腫脹,表面紅潤,無充血現(xiàn)象。免疫組化可見VEGF、VEGFR2蛋白在3組子宮內(nèi)膜上均有表達,平均光密度沒有明顯差異,但表達分布部位有差異。模型組相比于對照組,在基質(zhì)細胞和腺體上的表達分布較為廣泛。VEGFR2在子宮的肌層組織中表達有明顯差異:對照組幾乎沒有表達,模型組表達則明顯增高(P0.05),治療組比模型組表達有明顯下降(P0.05)。模型組子宮內(nèi)膜組織VEGF mRNA的表達是對照組的0.68倍,下降趨勢明顯;治療組是對照組的0.88倍,有上升趨勢。模型組子宮內(nèi)膜組織VEGFR2mRNA的表達是對照組的0.46倍,下降趨勢明顯;治療組是對照組的0.82倍,有比較明顯的上升趨勢。結(jié)論電針可以調(diào)整促排卵大鼠子宮組織中VEGF及其受體的表達,從而調(diào)整激素促排卵導致的子宮內(nèi)膜種植窗口期的同步性。
[Abstract]:Objective to observe the effect of electroacupuncture on the expression of VEGFR2 in uterine tissues of ovulatory rats induced by hormone, and to explore its mechanism. Methods Thirty female SD rats aged 10 weeks were randomly divided into three groups: control group, model group and treatment group. The model group and treatment group were injected with the same dose of human chorionic gonadotropin (hCGG) for 48 h after intraperitoneal injection of serum gonadotropin PMSG (PMSG) 20 U, and the mice were caged together with the mice. The experimental animals in the treatment group were continuously treated with electroacupuncture for 7 days (stimulation intensity 1 Ma, density wave 2 / 15 Hz) for 7 days (stimulation intensity 1 Ma, density wave 2 / 15 Hz). The experimental animals were killed on the 3.5 day after cage closure, and the uterine tissues were retained to observe the morphological changes, and the expression of VEGFFR2 protein was detected by immunohistochemical method. The expression of VEGFN VEGFR2 mRNA in uterine tissue was measured by qPCR technique. Results the uterine morphology of the control group was regular, the surface was ruddy and smooth, the uterine shape of the model group was enlarged and distorted, and the surface color was dark red and congestive. In the treatment group, the uterine shape was slightly swollen, the surface was ruddy, and there was no hyperemia. The expression of VEGF VEGFR2 protein was observed in the endometrium of the three groups with no significant difference in average optical density. Compared with the control group, the expression of VEGFR2 in stromal cells and glands in the model group was significantly different in the myometrium of uterus: there was almost no expression in the control group. The expression of VEGF mRNA in the model group was 0.68 times higher than that in the control group, and in the treatment group was 0.88 times higher than that in the control group, while the expression of VEGF mRNA in the model group was 0.68 times higher than that in the control group, while that in the treatment group was 0.88 times higher than that in the control group. The expression of VEGFR2mRNA in endometrium of the model group was 0.46 times of that of the control group, and decreased obviously, and the expression of VEGFR2mRNA in the treatment group was 0.82 times of that in the control group. Conclusion electroacupuncture can regulate the expression of VEGF and its receptors in uterine tissues of ovulatory rats, thus regulating the synchronism of endometrium implantation window induced by hormone ovulation.
【作者單位】： 南京中醫(yī)藥大學針藥結(jié)合重點實驗室;南京醫(yī)科大學第一臨床醫(yī)學院;
【基金】：國家自然科學基金(81403481,81473767) 江蘇省高校自然科學基金面上項目(14KJB360003)
【分類號】：R245

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本文編號：1523542