本文關(guān)鍵詞： 針灸預(yù)處理 衰老 脊髓 一氧化氮(NO) c-fos　出處：《錦州醫(yī)科大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

【摘要】：目的比較成年和老年大鼠對炎癥性疼痛反應(yīng)的差別,及針灸預(yù)處理對成年和老年福爾馬林致痛大鼠脊髓一氧化氮(Nitric oxide,NO)分布和c-fos表達(dá)的影響。方法將4月齡和21月齡雄性Sprague Dawley(SD)大鼠,分別隨機(jī)分為對照組(不做任何處理)、福爾馬林組(僅注射福爾馬林)、針灸足三里穴+福爾馬林組(針灸足三里穴后注射福爾馬林)、針灸非穴位+福爾馬林組(針灸非穴位后注射福爾馬林),每組6只大鼠。針灸處理后,除對照組外,其余各組于針灸位點(diǎn)同側(cè)足底注射福爾馬林。觀察大鼠行為學(xué)反應(yīng),記錄福爾馬林注射后1小時(shí)內(nèi)的縮足次數(shù)和舔/咬足時(shí)間(秒),1.5小時(shí)后灌流取脊髓第4腰髓(4th Lumbar spinal cord,L4)至第5腰髓(5th Lumbar spinal cord,L5)節(jié)段,采用酶組織化學(xué)染色和免疫組織化學(xué)染色法檢測脊髓NO的分布和c-fos的表達(dá)。結(jié)果1、對照組:NO數(shù)量較低,且老年大鼠脊髓背角NO數(shù)量低于成年大鼠(P0.01);Fos免疫陽性神經(jīng)元基礎(chǔ)水平較低,且在成年和老年大鼠間無差異(P0.05);成年和老年大鼠脊髓未發(fā)現(xiàn)NO和Fos雙標(biāo)陽性神經(jīng)元。2、福爾馬林組:老年大鼠繼發(fā)疼痛時(shí)相的縮足次數(shù)高于成年大鼠(P0.05);與對照組比較,NO陽性神經(jīng)元數(shù)、Fos免疫陽性神經(jīng)元數(shù)和二者雙標(biāo)陽性神經(jīng)元數(shù)在成年和老年大鼠脊髓均增加(P0.01),且老年大鼠的NO陽性神經(jīng)元數(shù)(P0.05,脊髓背角;P0.01,中央管)和Fos免疫陽性神經(jīng)元數(shù)(P0.01)高于成年大鼠。3、針灸足三里穴+福爾馬林組:成年和老年大鼠繼發(fā)疼痛時(shí)相的縮足次數(shù)(P0.01)和舔/咬足時(shí)間(成年,P0.05;老年,P0.01)低于福爾馬林組;成年和老年大鼠脊髓NO陽性神經(jīng)元數(shù)、Fos免疫陽性神經(jīng)元數(shù)和二者雙標(biāo)陽性神經(jīng)元數(shù)均低于福爾馬林組(P0.01);且老年組NO陽性神經(jīng)元數(shù)和Fos免疫陽性神經(jīng)元較福爾馬林組下降的幅度高于成年組大鼠(P0.01)。4、針灸非穴位+福爾馬林組:成年和老年大鼠繼發(fā)疼痛時(shí)相的縮足次數(shù)、舔/咬足時(shí)間、NO陽性神經(jīng)元數(shù)、Fos免疫陽性神經(jīng)元數(shù)和二者雙標(biāo)陽性神經(jīng)元數(shù)與福爾馬林組比較無差異(P0.05)。結(jié)論老年大鼠對炎癥性疼痛的反應(yīng)強(qiáng)于成年大鼠,針灸預(yù)處理足三里穴可以提高大鼠痛閾,減輕疼痛反應(yīng),降低脊髓NO的數(shù)量和c-fos的表達(dá),且對老年大鼠的作用優(yōu)于成年大鼠。
[Abstract]:Objective to compare the response of adult and aged rats to inflammatory pain. The effects of acupuncture and moxibustion preconditioning on the distribution of nitric oxide nitric oxide (no) and the expression of c-fos in spinal cord of adult and aged formalin-induced pain rats were studied. They were randomly divided into the control group (without any treatment), the formalin group (only injected with formalin, the acupuncture and moxibustion Zusanli point formalin group (acupuncture and moxibustion at Zusanli point after injection of formalin), the acupuncture and moxibustion non-acupoint formalin group (Acupuncture and moxibustion non-acupoint formalin group). After acupuncture and moxibustion, 6 rats in each group were injected with formalin after acupuncture and moxibustion. After acupuncture and moxibustion treatment, Except for the control group, the other groups were injected with formalin on the ipsilateral plantar of the acupuncture and moxibustion site. The number of foot contractions and the time of licking / biting were recorded within 1 hour after formalin injection (the 4th Lumbar spinal cordlordant L4 of the 4th Lumbar spinal of the spinal cord was perfused after 1.5 hours) to the 5th Lumbar spinal cordlord-L5 segment of the spinal cord. Enzyme histochemical staining and immunohistochemical staining were used to detect the distribution of no and c-fos expression in spinal cord. The basic level of Fos immunoreactive neurons in the spinal dorsal horn of aged rats was lower than that in adult rats. There was no difference between adult rats and old rats (P 0.05); no and Fos double labeled positive neurons were found in the spinal cord of adult and old rats. In formalin group, the number of foot contraction in the secondary pain phase of the aged rats was higher than that in the adult rats, and compared with the control group. The number of Fos immunoreactive neurons and the number of double labeled neurons increased in the spinal cord of adult and aged rats, and the number of no positive neurons in aged rats was P0.05, P0.01 in dorsal horn of spinal cord, central canal) and Fos immunized positive neurons. The number of sex neurons (P0.01) was higher than that of adult rats (P0.01), and the number of feet contraction (P0.01) and the time of licking / biting (P0.05; P0.01) were lower in the acupuncture and moxibustion Zusanli (Zusanli) group than in the formalin group. The number of Fos immunoreactive neurons and the number of double labeled neurons in spinal cord of adult and aged rats were lower than those of formalin group (P 0.01), and the number of no positive neurons and Fos immunoreactive neurons in the aged group was higher than that in formalin group. The decrease in the group was higher than that in the adult group (P 0.01), and that in the acupuncture and moxibustion non-acupoint formalin group: the number of foot contractions in the stage of secondary pain in adult and aged rats, There was no difference in the number of Fos immunoreactive neurons and the number of double labeled neurons between the two groups compared with formalin group. Conclusion the response to inflammatory pain in aged rats is stronger than that in adult rats. Acupuncture and moxibustion pretreatment at Zusanli point could increase the pain threshold, alleviate the pain response, decrease the number of no and c-fos expression in spinal cord, and the effect on aged rats was better than that of adult rats.
【學(xué)位授予單位】：錦州醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R245

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