發(fā)布時(shí)間：2018-02-09 23:26

  本文關(guān)鍵詞： 針康法 慢性低灌注 認(rèn)知功能 少突膠質(zhì)細(xì)胞　出處：《黑龍江中醫(yī)藥大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:通過觀察針康法對(duì)慢性低灌注大鼠認(rèn)知功能、腦白質(zhì)組織染色情況、胼胝體部位OLGs表達(dá)的影響,擬闡明針康法改善慢性低灌注導(dǎo)致的認(rèn)知功能障礙的可能機(jī)制。方法:30只健康Wistar雄性大鼠隨機(jī)分為假手術(shù)組(Sha),模型組(Mod),康復(fù)組(Reh),針刺組(Acu)及針康組(A+R),每組6只。除假手術(shù)組外,其它各組均采用雙側(cè)頸總動(dòng)脈永久結(jié)扎法(2-V0)制備慢性低灌注模型;假手術(shù)組僅給予分離雙側(cè)頸總動(dòng)脈(common carotid artery,CCA),既不結(jié)扎又不剪斷。經(jīng)過4周的頭穴叢刺結(jié)合康復(fù)訓(xùn)練后,Morris水迷宮檢測(cè)大鼠的認(rèn)知功能,HE染色觀察大鼠腦白質(zhì)組織的形態(tài)學(xué)變化,免疫組化法檢測(cè)大鼠胼胝體部位少突膠質(zhì)細(xì)胞(OLGs)陽性數(shù)目的表達(dá)。結(jié)果:1.學(xué)習(xí)記憶能力改變:慢性低灌注造模后,Morris水迷宮評(píng)測(cè)大鼠逃避潛伏期延長(zhǎng)、原平臺(tái)搜索次數(shù)減少,與Sha組相比差異有統(tǒng)計(jì)學(xué)意義(P0.05)。經(jīng)過4周干預(yù)后,Reh、Acu及A+R三組逃避潛伏期縮短、原平臺(tái)搜索次數(shù)增多,與Mod組相比差異有統(tǒng)計(jì)學(xué)意義(P0.05),且A+R組的Morris水迷宮評(píng)測(cè)成績(jī)優(yōu)于Reh、Acu組。2.HE染色觀察:Sha組大鼠的腦白質(zhì)無明顯的病理學(xué)改變;Mod組大鼠腦白質(zhì)出現(xiàn)不同程度的病理學(xué)改變:呈現(xiàn)皮層下、胼胝體、腦室旁白質(zhì)疏松,纖維排列凌亂,胼胝體出現(xiàn)空泡樣改變,皮質(zhì)下可見較大的軟化灶等;Reh、Acu及A+R三組大鼠腦白質(zhì)病理學(xué)改變較輕,神經(jīng)纖維排列緊密,胼胝體空泡面積較少,與Mod組相比差異明顯,且A+R組胼胝體空泡面積明顯減少,排列更加緊密規(guī)則。3.免疫組化檢測(cè)結(jié)果:頭穴叢刺治療和跑臺(tái)訓(xùn)練干預(yù)均能夠升高慢性低灌注大鼠胼胝體組織中OLGs的表達(dá),Reh、Acu及A+R組與Mod組相比,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。A+R組OLGs的表達(dá)高于Reh組及Acu組,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:1.針康法能改善慢性低灌注大鼠的認(rèn)知功能,且優(yōu)于單純針刺治療與單純康復(fù)治療。2.針康法可以明顯改善慢性低灌注大鼠腦白質(zhì)的病理學(xué)改變。3.針康法可以增加慢性低灌注大鼠腦白質(zhì)OLGs的表達(dá)。
[Abstract]:Objective: to observe the effects of acupuncture therapy on cognitive function, white matter staining and OLGs expression in corpus callosum of chronic hypoperfusion rats. Objective to elucidate the possible mechanism of acupuncture therapy in improving cognitive dysfunction induced by chronic hypoperfusion. Methods 30 healthy male Wistar rats were randomly divided into sham-operated group, model group, Rehor group, acupuncture group (Acua) and acupuncture group (6 rats in each group). Except for the sham-operation group, The chronic hypoperfusion model was established by permanent ligation of bilateral common carotid artery (2-V0) in all other groups. In the sham operation group, the common carotid artery of bilateral common carotid artery was isolated and neither ligated nor cut off. After 4 weeks of scalp acupuncture combined with rehabilitation training, Morris water maze was used to detect the cognitive function and HE staining of rat brain white matter tissue. Immunohistochemical method was used to detect the expression of oligodendrocyte oligodendrocytes (OLGs) in corpus callosum of rats. Results: 1. The ability of learning and memory: the latency of escape was prolonged and the search times of the original platform were decreased after Morris water maze was used to evaluate the rats' ability of learning and memory. After 4 weeks of intervention, the escape latency of the three groups was shortened, and the search times of the original platform increased, compared with that in the Sha group. Compared with Mod group, the difference was statistically significant (P 0.05), and the results of Morris water maze evaluation in group A and R were better than those in group A and R. 2. Observation of white matter in white matter of rats in group A and R with HE staining. There were no obvious pathological changes in the white matter of brain in rats in group A and R. There were pathological changes in the white matter of brain of rats in group A and R. Change: subcortical, In the corpus callosum, the white matter of the ventricle was loose, the fibers were disorganized, the corpus callosum appeared vacuolar changes, the subcortical soft foci were large, and the pathological changes of the white matter in the brain were mild and the nerve fibers were closely arranged in the three groups. The area of corpus callosum vacuole was less than that of Mod group, and the vacuolar area of corpus callosum was significantly decreased in AR group. The results of immunohistochemical examination showed that the expression of OLGs in the corpus callosum of rats with chronic hypoperfusion was increased by the treatment of scalp acupuncture and treadmill training. The expression of OLGs in the corpus callosum of rats with chronic hypoperfusion was higher than that in group A and R, as compared with that in group Mod. The expression of OLGs in P0.05A / R group was significantly higher than that in Reh group and Acu group, and the difference was statistically significant (P 0.05). Conclusion the acupuncture therapy can improve the cognitive function of rats with chronic hypoperfusion. Acupuncture therapy can significantly improve the pathological changes of the white matter of chronic hypoperfusion rats. Acupuncture Kang method can increase the expression of OLGs in the white matter of chronic hypoperfusion rats.
【學(xué)位授予單位】：黑龍江中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R245

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