本文關(guān)鍵詞： 穴位注射中藥茶去勢大鼠 SIRT1 衰老　出處：《貴州醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:觀察穴位注射和中藥茶對(duì)去勢大鼠內(nèi)分泌和沉默信息調(diào)節(jié)因子2相關(guān)酶1(SIRT1)水平的影響,以探討其治療更年期綜合征、延緩更年期衰老的作用機(jī)制。方法:選取3月齡雌性未孕SD大鼠156只,體重(250g±20g),為了更貼切地模擬人類更年期卵巢漸衰的過程,將其隨機(jī)分為假手術(shù)組以及三大組群:次切組群、次全切組群和全切組群(每個(gè)組群里分別包含:模型組、中藥茶組、穴位注射組和中藥茶+穴位注射組四個(gè)亞組),共13組,每組各12只大鼠。運(yùn)用次切、次全切、全切三種去勢手術(shù)方法造模,次切:右側(cè)卵巢全部切除,左側(cè)卵巢只切除約50%;次全切:右側(cè)卵巢全部切除,左側(cè)卵巢只切除約80%;全切:雙側(cè)卵巢全部切除。成模后,中藥茶組自由飲藥,共飲用8周。穴位注射組選取關(guān)元、歸來(雙)、肝俞(雙)、腎俞(雙),穴位注射丹紅注射液0.02mL/穴,1周1次,共8次。末次治療結(jié)束后,采用ELISA法檢測各組大鼠血清雌二醇(E2)、卵泡刺激素(FSH)以及心臟組織SIRT1蛋白的水平;取大鼠子宮組織HE染色后觀察其形態(tài)結(jié)構(gòu)的變化。結(jié)果:與假手術(shù)組相比,各模型組大鼠血清E2水平和心臟組織SIRT1蛋白的水平均顯著降低,血清FSH的水平顯著升高,其差異均具有統(tǒng)計(jì)學(xué)意義(P0.05);與模型組相比,各治療組大鼠血清E2水平和心臟組織SIRT1蛋白的水平均顯著升高,血清FSH的水平均顯著降低,其差異均具有統(tǒng)計(jì)學(xué)意義(P0.05);其中,次切中藥茶+穴位注射組大鼠的E2水平和SIRT1蛋白水平均值最高,FSH水平均值最低。大鼠子宮內(nèi)膜形態(tài)學(xué)觀察結(jié)果提示:各治療組均能使大鼠子宮內(nèi)膜上皮增厚,使間質(zhì)致密、腺體增多、血管豐富,其中,以次切中藥茶+穴位注射組治療效果最佳。結(jié)論:(1)本實(shí)驗(yàn)運(yùn)用次切、次全切、全切三種去勢手術(shù)方法造模,成功地復(fù)制了更年期大鼠模型,并模擬了人類更年期卵巢漸衰的過程。(2)穴位注射和中藥茶能延緩去勢大鼠衰老進(jìn)程,其作用機(jī)制可能是穴位注射和中藥茶能提高去勢大鼠血清E2水平以及心臟組織SIRT1蛋白的水平,降低血清FSH水平。(3)各治療組間的數(shù)據(jù)結(jié)果提示:在大鼠卵巢漸衰的過程中,早期聯(lián)合干預(yù)治療療效更佳。
[Abstract]:Objective: to observe the effects of acupoint injection and traditional Chinese medicine tea on endocrine and silent information regulatory factor-2 related enzyme 1SIRT1 in ovariectomized rats, in order to explore the treatment of climacteric syndrome. Methods: 156 3-month-old female non-pregnant SD rats, weighing 250g 鹵20g / g, were selected to simulate the process of ovarian decline in human menopause. It was randomly divided into sham-operation group and three major groups: subtotal group, subtotal group and total group (each group included: model group, traditional Chinese medicine tea group). There were 12 rats in each of 13 groups of rats in the acupoint injection group and the Chinese herbal tea point injection group. The models were made by three castration methods: total ovariectomies and ovariectomies. The left ovary was excised only about 50 times. Subtotal excision: the right ovary was excised completely, the left ovary was removed only about 80%; Total resection: bilateral ovariectomies. After the model, the traditional Chinese medicine tea group drank freely for 8 weeks. The acupoint injection group selected Guan Yuan and returned (Shuangli, Ganshu (Shuangyou, Shenshu)). Point injection of Danhong injection 0.02mL / d once a week, a total of 8 times. After the end of the last treatment, the serum estradiol estradiol E2 was detected by ELISA method in each group. Follicle stimulating hormone (FSH) and the level of SIRT1 protein in heart tissue; Results: compared with the sham-operated group, the serum E2 level and the SIRT1 protein level in the heart tissue of the model group were significantly lower than those of the sham operation group. The level of serum FSH was significantly increased, and the difference was statistically significant (P 0.05). Compared with the model group, the levels of serum E2 and SIRT1 protein in heart tissue of each treatment group were significantly increased, and the serum FSH levels were significantly decreased. The differences were statistically significant (P 0.05). Among them, the level of E _ 2 and the average value of SIRT1 protein were the highest in the injection group. The average value of FSH water was the lowest. The results of morphological observation of rat endometrium showed that each treatment group could make endometrial epithelium thicker, make interstitial dense, increase glands and enrich blood vessels. Conclusion: in this experiment, we used three castration methods: secondary, subtotal and total resection to make models, and successfully duplicated the model of climacteric rats. And simulated the process of human menopausal ovarian decline. 2) Acupoint injection and traditional Chinese medicine tea can delay the aging process of ovariectomized rats. The mechanism may be that acupoint injection and Chinese herbal tea can increase the level of serum E2 and SIRT1 protein in cardiac tissue of ovariectomized rats. The results showed that the early combined intervention was more effective in the process of ovarian decline in rats.
【學(xué)位授予單位】：貴州醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R245

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張紅艷;岑加萍;黃堅(jiān);鄧妙;劉元偉;張治芬;;雌激素對(duì)去勢大鼠SIRT1表達(dá)的影響[J];國際生殖健康/計(jì)劃生育雜志;2015年02期

2 曾春燕;葉云;;激素替代療法治療更年期綜合征的研究進(jìn)展[J];中國藥房;2014年18期

3 劉姍姍;潘五九;王偉明;;中藥治療女性更年期綜合癥作用機(jī)理研究現(xiàn)狀[J];黑龍江中醫(yī)藥;2014年01期

4 李瑞奇;白明;苗明三;;更年期綜合征動(dòng)物模型的特點(diǎn)及研究展望[J];中醫(yī)學(xué)報(bào);2012年06期

5 于舒雁;劉會(huì)麗;苗明三;;更年期綜合征的分子機(jī)制及中醫(yī)藥治療更年期綜合征的特點(diǎn)[J];中醫(yī)學(xué)報(bào);2012年03期

6 高苒;;激素替代治療女性更年期綜合征[J];中國醫(yī)藥指南;2011年08期

7 王嵐;梁日欣;楊濱;楊艷;葉錦霞;殷小杰;楊慶;王彥禮;;黃芩及紅花水提物對(duì)快速老化模型小鼠的抗衰老作用研究[J];中國實(shí)驗(yàn)方劑學(xué)雜志;2010年13期

8 崔清志;田艷君;溫海霞;張國棟;韓蕊娜;張廣美;劉國藝;;益坤寧對(duì)圍絕經(jīng)期大鼠卵巢細(xì)胞凋亡率及caspase-3表達(dá)的影響[J];中國應(yīng)用生理學(xué)雜志;2010年03期

9 馬曉艷;白文佩;胡麗娜;;黑升麻治療絕經(jīng)綜合征的作用機(jī)制探討[J];實(shí)用婦產(chǎn)科雜志;2010年06期

10 宋曉琳;李曉泓;徐莉莉;王洪彬;;熱休克蛋白與圍絕經(jīng)期綜合征防治相關(guān)性研究[J];中華中醫(yī)藥雜志;2010年01期

相關(guān)博士學(xué)位論文 前1條

1 潘家怡;雷射針灸治療肝腎陰虛型更年期綜合征的臨床研究[D];南京中醫(yī)藥大學(xué);2011年

相關(guān)碩士學(xué)位論文 前6條

1 李鵬飛;鎖陽總黃酮干預(yù)大鼠圍絕經(jīng)期模型的分子機(jī)制[D];河南中醫(yī)學(xué)院;2015年

2 張紅艷;雌激素對(duì)去勢大鼠SIRT1影響的研究[D];南京醫(yī)科大學(xué);2015年

3 魏珍珍;肉蓯蓉苯乙醇苷對(duì)大、小鼠圍絕經(jīng)期模型的影響[D];河南中醫(yī)學(xué)院;2014年

4 汪怡新;針?biāo)幗Y(jié)合治療圍絕經(jīng)期綜合征的臨床觀察及實(shí)驗(yàn)研究[D];南京中醫(yī)藥大學(xué);2014年

5 李斯;電芒針治療女性更年期綜合征的臨床隨機(jī)對(duì)照研究[D];北京中醫(yī)藥大學(xué);2013年

6 彭娟;益婦寧延緩大鼠卵巢衰老的藥效與機(jī)制研究[D];南方醫(yī)科大學(xué);2013年

，

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