本文關(guān)鍵詞：香附炮制前后揮發(fā)油的GC-MS指紋圖譜對比研究，由筆耕文化傳播整理發(fā)布。

香附炮制前后揮發(fā)油的GC-MS指紋圖譜對比研究

Comparative study on GC-MS fingerprints of volatile oil in crude and processed Cyperi Rhizoma

[1] [2] [3] [4] [5] [6] [7]

SHENG Fei-ya, LU Jun-rong, PENG Wei, FU Chao-mei, ZHANG Le-le, LIU Fang, WANG Shi-yu State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of

成都中醫(yī)藥大學(xué)中藥材標準化教育部重點實驗室中藥資源系統(tǒng)研究與開發(fā)利用省部共建國家重點實驗室培育基地,四川成都611137

文章摘要：目的建立香附生品、參照品和醋制品的揮發(fā)油GC—MS指紋圖譜，擬從整體角度表征香附炮制前后化學(xué)信息的變化。方法采用GC—MS法，建立了3組香附揮發(fā)油的指紋圖譜，并采用中藥色譜指紋圖譜相似度評價軟件A版進行數(shù)據(jù)分析。結(jié)果香附各制品相似度良好，驗證了前期篩選的炮制工藝穩(wěn)定可行。得到了香附生品共有峰52個、參照品28個、醋制品53個，鑒定了其中46個峰。對比分析發(fā)現(xiàn)，，香附炮制后其揮發(fā)油成分種類、質(zhì)量分數(shù)均有所變化；以生品為參照，對比參照品和醋制品共有成分，結(jié)果醋制品揮發(fā)油成分種類保留更為完整。結(jié)論以醋作為炮制輔料科學(xué)合理，可起到穩(wěn)定保護香附揮發(fā)油成分的作用，為揭示香附醋制機制及其物質(zhì)基礎(chǔ)提供一定科學(xué)依據(jù)。

Abstr：Objective To establish the GC-MS fingerprints of volatile oil in crude, water-processed, and vinegar-processed Cyperi Rhizoma, and to capture the whole scope of the chemical constituents changing. Methods The fingerprints of volatile oil in crude, water-processed, and vinegar-processed Cyperi Rhizoma were established by GC-MS. And the data were analyzed by chromatographic fingerprint similarity evaluation software for Chinese materia medica （Version A）. Results The similarities of each group were good, which indicated that the technology was stable and feasible. The crude group gained 52 common peaks, 28 as the water-processed group, and 53 as the vinegar-processed group. Meanwhile, 46 chemical components among the peaks above were identified in total. Comparing with the three groups, the kinds and contents of many components in each group were changed after processing. With the crude group as reference, the differences between the water-processed group and vinegar-processed group were contrastively analyzed before and after processing, and the Cyperi Rhizoma processed with vinegar could remain the chemical constitutions in its volatile oil better. Conclusion Using vinegar as processing accessory is scientific and reasonable. Vinegar could play a role in protecting the compounds of volatile oil in Cyperi Rhizoma steadily.

文章關(guān)鍵詞：

Keyword：:Cyperi Rhizoma processing volatile oil GC-MS fingerprint

課題項目：國家自然科學(xué)基金資助項目（81303227）

  本文關(guān)鍵詞：香附炮制前后揮發(fā)油的GC-MS指紋圖譜對比研究，由筆耕文化傳播整理發(fā)布。