本文關(guān)鍵詞： 復(fù)方地萸中空栓 圍絕經(jīng)期綜合征 性激素 β-內(nèi)啡肽　出處：《山西省中醫(yī)藥研究院》2016年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:通過制備中藥中空栓(復(fù)方地萸中空栓)作用于圍絕經(jīng)期綜合征大鼠模型后檢測(cè)相關(guān)指標(biāo),從分子水平探討復(fù)方地萸中空栓對(duì)圍絕經(jīng)期綜合征的影響及作用機(jī)制,為進(jìn)一步研究及臨床使用奠定基礎(chǔ)。方法:1取復(fù)方中熟地、山茱萸、黃連、丹參、鉤藤、香附、山藥、茯神、遠(yuǎn)志、百合、葛根、浮小麥、甘草共13味藥的免煎顆粒放入研缽內(nèi)進(jìn)行研磨,過120目篩,得復(fù)方免煎顆粒藥粉,通過文獻(xiàn)、導(dǎo)師前期研究成果及相關(guān)實(shí)驗(yàn)確定的具體制備方法,制備出大鼠用中藥中空栓每粒約重1.905g,含復(fù)方免煎顆粒藥量0.333g。1選48只生育齡雌性未孕SD大鼠,隨機(jī)分成假手術(shù)組、模型組、陽性組、中藥灌胃組、中藥中空栓高劑量組、中藥中空栓低劑量組。除假手術(shù)組背部切口切除少量脂肪組織后立即縫合切口皮膚外,其余5組大鼠手術(shù)切除雙側(cè)卵巢,制備圍絕經(jīng)期綜合征大鼠模型。各組大鼠術(shù)后恢復(fù)性喂養(yǎng)一周,并予前三天連續(xù)肌肉注射青霉素4萬個(gè)單位,1次/天,以防感染。一周后,各組大鼠進(jìn)行陰道細(xì)胞學(xué)涂片觀察,1次/天,連續(xù)五天,剔除去勢(shì)不徹底(造模不成功)的大鼠。1陰道涂片檢查后,假手術(shù)組及模型組給予空白中空栓,陽性組給予戊酸雌二醇中空栓,中藥灌胃組灌服復(fù)方地萸混懸液,中藥中空栓高劑量組與低劑量組分別給予相應(yīng)劑量的復(fù)方地萸中空栓。連續(xù)給藥14天,末次給藥后24小時(shí)準(zhǔn)備殺檢,殺檢前12個(gè)小時(shí)禁食不禁水,腹主動(dòng)脈取血,迅速斷頭處死大鼠,摘取下丘腦。用Elisa試劑盒測(cè)定血清性激素雌二醇(E_2)、卵泡刺激素(FSH)、黃體生成素(LH)及下丘腦β-內(nèi)啡肽(β-EP)。結(jié)果:1復(fù)方地萸中空栓能夠提高大鼠血清E_2水平的同時(shí)降低FSH、LH的水平,較模型組有顯著差異(P0.05),較陽性組、中藥灌胃組無顯著差異(P0.05);1復(fù)方地萸中空栓能夠提高下丘腦β-EP含量,較模型組有顯著差異(P0.05),較陽性組、中藥灌胃組無顯著差異(P0.05)。結(jié)論:復(fù)方地萸中空栓能調(diào)節(jié)圍絕經(jīng)綜合征大鼠模型血清性激素水平及下丘腦β-EP的分泌,使E_2、β-EP升高,FSH、LH降低,從而改善圍絕經(jīng)期綜合征的臨床癥狀。其機(jī)制可能是通過調(diào)節(jié)這些指標(biāo)直接或間接參與調(diào)節(jié)圍絕經(jīng)期綜合征神經(jīng)內(nèi)分泌系統(tǒng)功能。
[Abstract]:Objective: to detect the related indexes after the preparation of traditional Chinese medicine hollow suppository (compound Diyu hollow suppository) on the model of peri-menopausal syndrome in rats. To explore the effect and mechanism of compound Diyu hollow suppository on peri-menopausal syndrome from molecular level, and to lay a foundation for further study and clinical application. Methods: 1. Take compound Corni officinalis, Fructus Corni, Coptis chinensis and Salvia miltiorrhiza (Salvia miltiorrhiza). Uncaria, fragrant, yam, Fu Shen, Yuan Zhi, Lily, Pueraria root, floating wheat, licorice a total of 13 kinds of medicine granules into the mortar for grinding, after 120 mesh sieve, obtained compound Fan-Fen granule powder, through the literature. The former research results and the specific preparation methods determined by related experiments, the preparation of Chinese medicine hollow suppository for rats weighs about 1.905g. 48 female SD rats with reproductive age were randomly divided into sham-operation group, model group, positive group, Chinese medicine gavage group and high dose group of Chinese medicine hollow suppository. The low-dose group of hollow suppository: except for the sham operation group, the skin of the incision was sutured immediately after a small amount of fat tissue was removed from the back of the sham operation group, and the other 5 groups of rats were operated to remove bilateral ovaries. The model of rats with peri-menopausal syndrome was established. The rats in each group were fed for one week after operation, and penicillin was injected intramuscularly once a day for three days to prevent infection. The vaginal cytologic smears were observed once a day for five consecutive days. After removing the castrated rats, the sham operation group and the model group were given blank hollow suppository. The positive group was given estradiol valerate hollow suppository, the Chinese medicine gavage group was infused with compound Diyu suspension, the high dose group and the low dose group of Chinese medicine hollow suppository were given the corresponding dose of compound diyu hollow suppository for 14 days. The rats were killed 24 hours after the last administration. The rats were killed by fasting 12 hours before the last administration. Blood was taken from the abdominal aorta and the rats were killed quickly. Hypothalamus was removed. Serum estradiol estradiol (E _ 2) and follicle stimulating hormone (FSH) were determined by Elisa kit. Results the hypothalamus 尾 -endorphin (尾 -EP) and luteinizing hormone (LH) could increase the serum E2 level and decrease the FSHLH level in rats. Compared with the model group, there was a significant difference between the two groups, but there was no significant difference between the Chinese medicine group and the positive group. 1 compound Diyu hollow suppository could increase 尾 -EP content in hypothalamus, which was significantly different from model group (P 0.05) and positive group (P < 0.05). Conclusion: compound Diyu hollow suppository can regulate the level of serum sex hormone and the secretion of 尾 -EP in hypothalamus of rats with peri-menopausal syndrome. 尾 -EP increased and FSH LH decreased. Therefore, the clinical symptoms of peri-menopausal syndrome can be improved. The mechanism may be to regulate the neuroendocrine system function of perimenopausal syndrome directly or indirectly by regulating these indexes.
【學(xué)位授予單位】：山西省中醫(yī)藥研究院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R271.116

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