本文關(guān)鍵詞： 針刺 原發(fā)性高血壓 代謝組學 液相色譜-質(zhì)譜分析　出處：《成都中醫(yī)藥大學》2016年碩士論文　論文類型：學位論文

【摘要】：目的：采用液相色譜-質(zhì)譜聯(lián)用(Liquid Chromatography-Mass Spectrometry, LC/MS)技術(shù),研究原發(fā)性高血壓(Essential Hypertension, EH)患者和健康受試者血漿代謝物的差別,并觀察針刺對EH患者血漿代謝物的影響。方法：1.EH患者的血漿代謝全譜分析：采用LC/MS技術(shù),分析EH患者與健康受試者血漿代謝物的差別,獲得EH關(guān)鍵代謝物。2.針刺治療EH的血漿代謝全譜分析：分別采用經(jīng)穴、非經(jīng)非穴兩種方法針刺治療EH患者,運用LC/MS代謝組學技術(shù),觀察針刺對EH患者血漿代謝物的影響以及組間差異。3.差異代謝物與臨床血壓相關(guān)性分析：采用24h動態(tài)血壓儀監(jiān)測患者全天的平均血壓,運用Pearson線性相關(guān)分析,篩選出與針刺治療EH療效相關(guān)的代謝物,找到EH的潛在生物標志物。結(jié)果：1.EH患者的血漿代謝全譜分析結(jié)果利用LC/MS技術(shù)能較好區(qū)分EH患者與健康受試者的代謝狀態(tài)。與健康對照組相比,EH患者血漿中差異m/z126.1030(3-甲基組氨酸)、530.2777(甘氨酸)、284.2949(鞘氨醇)等濃度顯著上升(P0.05)；差異m/z229.1176(門冬氨酸、亮氨酸、異亮氨酸)等濃度顯著下降(P0.05)。2.針刺治療EH的血漿代謝全譜分析結(jié)果(1)經(jīng)穴治療EH患者的臨床效應優(yōu)于非經(jīng)非穴組,并能顯著改善EH患者的24小時平均收縮壓、24小時平均舒張壓、日間平均收縮壓和日間平均舒張壓；非經(jīng)非穴針刺治療EH患者血壓降低不明顯,無統(tǒng)計學差異(P0.05)。(2)采用LC/MS技術(shù)能較好區(qū)分EH患者針刺治療前后以及針刺組間的代謝狀態(tài)。(3)經(jīng)穴組患者血漿中差異m/z387.1283(油酸)的濃度在針刺后顯著上升；非經(jīng)非穴組血漿中差異m/z265.1]75(苯丙氨酸)的濃度在針刺后顯著下降；與非經(jīng)非穴組相比,經(jīng)穴組患者治療后血漿代謝物中差異m/z450.2977(溶血磷脂酰膽堿LysoPC(14:0))濃度顯著上升(P0.05),差異m/z287.2362(花生四烯酸)濃度顯著下降(P0.05)。3.差異代謝物與臨床血壓相關(guān)性分析結(jié)果(1)受試者基線期血漿代謝物濃度與患者的24小時平均收縮壓、24小時平均舒張壓進行Pearson線性相關(guān)分析結(jié)果顯示,差異m/z126.1030(3-甲基組氨酸)與24h平均收縮壓顯著負相關(guān)(P0.05),530.2777(甘氨酸)、607.3059(膽酸葡萄糖醛酸)、284.2949(鞘氨醇)與24h平均舒張壓顯著正相關(guān)(P0.05)。(2)EH患者針刺前后血漿代謝物濃度變化值與患者24小時平均收縮壓、24小時平均舒張壓血壓變化值的Pearson線性相關(guān)分析結(jié)果顯示,差異m/z211.1441(脯氨酸)的改變值與24h平均收縮壓改變值顯著負相關(guān)(P0.05),差異m/z263.0896(脂肪酸：3-Carboxy-4-me thyl-5-propyl-2-furanpropionic acid)與24h平均舒張壓顯著正相關(guān)(P0.05),454.3150(脂肪酸：simvastatin hydroxy acid)與24h平均舒張壓顯著負相關(guān)(P0.05)。結(jié)論：1.EH患者血漿代謝全譜與健康對照組不同,主要集中在氨基酸代謝、脂代謝方面。2.經(jīng)穴與非經(jīng)非穴兩種治療方式對EH患者的血漿代謝物均有一定的調(diào)節(jié)作用,但調(diào)節(jié)的代謝物不同,EH患者血漿代謝物與臨床血壓存在一定相關(guān)性。
[Abstract]:Objective: to use liquid Chromatography-Mass spectrometric (LC / MS) technique by liquid chromatography-mass spectrometry (LC-MS). To study the difference of plasma metabolites between patients with essential hypertension (EH) and healthy subjects. To observe the effect of acupuncture on plasma metabolites in EH patients. Methods 1. Total spectrum analysis of plasma metabolism in EH patients. The difference of plasma metabolites between EH patients and healthy subjects was analyzed by LC/MS technique. Obtain essential metabolite of EH .2.The whole spectrum analysis of plasma metabolism in acupuncture treatment of EH: acupuncture at meridian and non-meridian points were used to treat EH patients, and LC/MS metabolomics technique was used to treat EH patients. To observe the effect of acupuncture on plasma metabolites of EH patients and the difference between groups. 3. Analysis of the correlation between different metabolites and clinical blood pressure: 24 hours ambulatory blood pressure instrument was used to monitor the mean blood pressure of the patients all day. Pearson linear correlation analysis was used to screen the metabolites related to the effect of acupuncture on EH. Find potential biomarkers for EH. Results:. 1. The results of plasma metabolic total spectrum analysis of EH patients were compared with those of healthy subjects. 2. LC/MS technique was used to distinguish the metabolic status between EH patients and healthy subjects. The difference in plasma levels between patients with EH was m / z126.1030m- methylhistidine 530.2777 (glycine). 284.2949 (sphingosine) etc. The difference was m / z 229.1176 (aspartate, leucine). The plasma metabolism of EH treated by acupuncture was significantly decreased (P0.05n.2.The results of total spectrum analysis of plasma metabolism of acupuncture) showed that the clinical effect of acupoint therapy on EH patients was better than that of non-acupoints group. The mean systolic blood pressure of 24 hours, the mean systolic blood pressure during day and the average diastolic blood pressure in day were significantly improved in patients with EH. There was no significant decrease in blood pressure in EH patients treated with non-meridian and non-acupoint acupuncture. There was no statistical difference (P 0.05). (2) LC/MS technique was used to distinguish the metabolic state of EH patients before and after acupuncture treatment and between acupuncture groups (P < 0.05). The difference in plasma concentration of m / z 387.1283 (oleic acid) in acupoint group increased significantly after acupuncture. The concentration of phenylalanine in the plasma of non-meridian and non-acupoint group decreased significantly after acupuncture, and the difference of m / z 265.1] 75 (phenylalanine) was observed. Compared with non-meridian non-acupoint group. After treatment, the difference of plasma metabolites in the meridian acupoint group was that the concentration of lysophosphatidylcholine (lysophosphatidylcholine, LysoPC14: 0) increased significantly (P0.05). The difference of m / z _ 287.2362 (arachidonic acid) concentration was significantly decreased (P _ (0.05) n 路3). The correlation between different metabolites and clinical blood pressure was (1). Baseline plasma metabolite concentrations and 24-hour mean systolic blood pressure in subjects. 24 hours mean diastolic blood pressure was analyzed by Pearson linear correlation analysis. The difference between m / z 126.1030 and 3-methylhistidine was negatively correlated with 24 h mean systolic blood pressure (P 0.05) and 530.2777 (glycine). 607.3059 (cholic acid glucuronic acid). 284.2949 (sphingosine) and 24h mean diastolic blood pressure were positively correlated with plasma metabolites concentration before and after acupuncture and 24 hours mean systolic blood pressure. Pearson linear correlation analysis of 24 hour mean diastolic blood pressure (DBP) changes showed. There was a significant negative correlation between the variation of proline and 24 h mean systolic blood pressure (P 0.05). Difference (fatty acid: 3-Carboxy-4-me thyl-5-propyl-2-furanpropionic acid). There was a significant positive correlation with 24 h mean diastolic blood pressure (P 0.05). 454.3150 (fatty acid: simvastatin hydroxy acid) was negatively correlated with 24-hour mean diastolic pressure (P 0.05). Conclusion: 1. The whole spectrum of plasma metabolism in EH patients is different from that in healthy controls. Mainly concentrated in the metabolism of amino acids, lipid metabolism. 2. Both meridian and non-meridian points have a certain regulatory effect on plasma metabolites in EH patients, but the regulated metabolites are different. Plasma metabolites were correlated with clinical blood pressure in EH patients.
【學位授予單位】：成都中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R246.1

【參考文獻】

相關(guān)期刊論文 前10條

1 李曉宇;朱旭冬;陳琪;;甘氨酸在心血管疾病中的保護作用[J];生物化學與生物物理進展;2015年09期

2 楊星月;馬玉俠;杜冬青;高樹中;;基于代謝組學的隔藥灸臍法治療原發(fā)性痛經(jīng)的機理研究[J];上海針灸雜志;2015年08期

3 周次利;陸Z，

本文編號：1462804