本文關(guān)鍵詞：藏藥“蒂達(dá)”的6種主要基原植物提取物對(duì)急性肝內(nèi)膽汁淤積小鼠藥理作用的比較研究　出處：《中藥新藥與臨床藥理》2017年03期 　論文類型：期刊論文

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【摘要】：目的比較藏藥"蒂達(dá)"(藏茵陳)的6種主要基原植物小傘虎耳草、篦齒虎耳草、印度獐牙菜、川西獐牙菜、大籽獐牙菜和橢圓葉花錨提取物對(duì)急性肝內(nèi)膽汁淤積小鼠的藥理作用,為其品種整理、質(zhì)量控制、臨床應(yīng)用及資源綜合開發(fā)利用提供藥理學(xué)依據(jù)。方法采用α-異硫氰酸萘酯(ANIT)誘導(dǎo)小鼠急性肝內(nèi)膽汁淤積型肝損傷模型,以各藥材提取物進(jìn)行干預(yù),熊去氧膽酸(UDCA)為對(duì)照,檢測小鼠血清ALT、AST、TBA、TBIL、DBIL和IBIL水平,HE染色觀察肝組織病理變化。結(jié)果 6種藥材提取物均能不同程度地改善ANIT引起的肝損傷,各藥材提取物組在肝小葉結(jié)構(gòu)、肝細(xì)胞病變等方面病理改變均相對(duì)減輕,其中,以小傘虎耳草組、篦齒虎耳草組、橢圓葉花錨組和大籽獐牙菜組明顯;各藥材提取物組小鼠血清中ALT和AST的水平均明顯降低,小傘虎耳草、印度獐牙菜和川西獐牙菜組小鼠血清中TBA的水平均明顯降低,小傘虎耳草、篦齒虎耳草、川西獐牙菜和大籽獐牙菜組小鼠血清中TBIL、DBIL和IBIL的水平均明顯降低。結(jié)論小傘虎耳草和篦齒虎耳草提取物在降酶和退黃兩方面效果最為顯著,且總體強(qiáng)于陽性藥熊去氧膽酸,是開發(fā)針對(duì)膽汁淤積型肝病治療藥物的優(yōu)勢藥材品種;6種藥材提取物均可明顯改善急性肝內(nèi)膽汁淤積小鼠肝組織損傷及肝功能,說明藏醫(yī)將其歸為同一類藥材存在一定的合理性,而其藥理作用差異則提示藏藥"蒂達(dá)"各基原藥材間在功效、物種親緣關(guān)系及化學(xué)成分方面存在差異和相關(guān)關(guān)系;本研究為"蒂達(dá)"藥材的品種整理、質(zhì)量控制、臨床應(yīng)用和資源綜合開發(fā)利用提供了藥理學(xué)依據(jù),并為其他類似復(fù)雜基原藏藥的品種整理等研究提供了參考。
[Abstract]:Objective to compare the six main basic plants of Tibetan medicine "Tida", Saxifraga grisea, Swertia davidiana and Swertia mustiflorum. The pharmacological effects of Swertia davidiflora and Anchorage ovale extracts on acute intrahepatic cholestasis mice were studied. Methods the acute intrahepatic cholestatic liver injury model in mice was induced by 偽 -naphthalene thiocyanate. The serum levels of TBILIL and IBIL were measured in mice with ursodeoxycholic acid (UDCA). Results the liver injury induced by ANIT was improved in all the 6 kinds of extracts, and the structure of hepatic lobule was found in each extract group. The pathological changes of liver cell were relatively alleviated, among which, the group of Saxifraga vulgaris, the group of Saxifraga grisea, the group of Anchorage of elliptical leaf and the group of Swertia davidiflora were obvious. The levels of ALT and AST in the serum of mice in the extract group were significantly lower than those in the control group. The levels of TBA in the serum of the mice in the three groups were significantly lower than those in the control group. TBIL in serum of mice in the groups of Saxifraga grisea Swertia and Swertia davidii. The levels of DBIL and IBIL were significantly decreased. Conclusion the extract of Saxifraga vulgaris and Saxifraga grisea has the most significant effect in reducing enzyme and reducing yellow, and it is stronger than ursodeoxycholic acid in general. Is the development of cholestatic liver disease treatment drugs for the advantage of the variety; The six kinds of extracts could obviously improve the liver tissue injury and liver function in mice with acute intrahepatic cholestasis, which indicated that it was reasonable for Tibetan medicine to classify them into the same kind of medicine. The difference of pharmacological action indicated that there were differences and correlations in efficacy, species relationship and chemical composition among the original medicinal materials of Tibetan medicine "Tida". This study provides pharmacological basis for the variety arrangement, quality control, clinical application and comprehensive exploitation and utilization of Tida, and provides a reference for the variety arrangement of other similar complex primitive Tibetan medicines.
【作者單位】： 江西中醫(yī)藥大學(xué);江西民族傳統(tǒng)藥現(xiàn)代科技與產(chǎn)業(yè)發(fā)展協(xié)同創(chuàng)新中心;重慶市中藥研究院;
【基金】：國家自然科學(xué)基金(81360675) 國家科技重大專項(xiàng)“重大新藥創(chuàng)制”子課題(2012ZX09103201-050)
【分類號(hào)】：R29
【正文快照】： 膽汁淤積型肝病是以膽汁淤積為主要表現(xiàn)的臨床常見疾病,是各種原因引起的膽汁形成、分泌和(或)膽汁排泄異常引起的肝臟病變。熊去氧膽酸(UDCA)為治療膽汁淤積型肝病的首選藥物[1-2]�！暗龠_(dá)”(藏茵陳)為藏醫(yī)臨床治療肝膽疾病使用頻率最高的一類藥材,《晶珠本草》[3]中記載:“

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