本文關(guān)鍵詞：電針足三里、合谷穴對IBS模型大鼠腸道痛敏及動力異常治療效應(yīng)的比較研究　出處：《北京中醫(yī)藥大學(xué)學(xué)報》2017年06期 　論文類型：期刊論文

  更多相關(guān)文章： 腸易激綜合征 合谷穴 足三里穴 內(nèi)臟痛 胃腸動力 經(jīng)穴效應(yīng)特異性 大鼠

【摘要】：目的探討不同經(jīng)脈及不同神經(jīng)節(jié)段支配的穴位對同一疾病不同癥狀的療效差異及相關(guān)機制。方法新生Wistar大鼠隨機分為空白組、模型組、足三里組和合谷組,每組10~12只。除空白組外,均采用母子分離加醋酸灌腸結(jié)合結(jié)直腸擴張(CRD)聯(lián)合制備腸易激綜合征(IBS)模型。造模成功者,2月齡時,足三里組、合谷組給予相應(yīng)穴位電針刺激,20 min/次,隔日1次,共5次。觀察各組大鼠電針前后的糞便性狀,采用Bristol分型標準評分;腹部回撤反射(AWR)評價內(nèi)臟痛覺敏感性;免疫組化法檢測結(jié)腸辣椒素受體(TRPV1)、5-HT2AR的陽性表達。結(jié)果與空白組比較,針刺前,模型組、足三里組及合谷組大鼠的Bristol評分均顯著升高(P0.01);針刺后,模型組(P0.01)、合谷組(P0.05)大鼠的評分升高;模型組、足三里組腹抬壓力閾值明顯降低(P0.01),收縮波明顯增加(P0.01),結(jié)腸TRPV1陽性表達均升高(P0.01),合谷組結(jié)腸TRPV1表達增高(P0.05)。模型組、合谷組結(jié)腸5-HT2AR陽性表達升高(P0.01)。與模型組比較,足三里組及合谷組,針刺后Bristol大便評分降低(P0.01);腹抬壓力閾值升高(P0.01)、收縮波個數(shù)減少(P0.01);結(jié)腸TRPV1、5-HT2AR陽性表達均顯著性降低(P0.01)。與足三里組相比,合谷組針后Bristol評分升高(P0.05),結(jié)腸TRPV1表達下降(P0.01),5-HT2AR表達升高(P0.01)。結(jié)論電針足三里穴和合谷穴均能降低IBS大鼠腸道痛敏感性,改善胃腸動力障礙。兩穴比較,合谷穴對IBS內(nèi)臟痛的治療效應(yīng)略優(yōu)于足三里穴,而足三里穴對胃腸運動的調(diào)節(jié)效應(yīng)更具優(yōu)勢。說明不同經(jīng)脈、不同神經(jīng)節(jié)段支配的穴位可以治療同一病癥,但存在效應(yīng)差異,證實了經(jīng)穴效應(yīng)特異性存在且具有相對性。
[Abstract]:Objective to explore the therapeutic effects and related mechanisms of different acupoints innervated by different meridians and different nerve segments on the same disease and different symptoms. Methods newborn Wistar rats were randomly divided into blank group and model group. In Zusanli group and Hegu group, 12 rats were in each group, except the blank group. The IBS model of irritable bowel syndrome (IBS) was established by the combination of mother and child separation with acetic acid enema combined with colorectal dilatation (CRD). The successful rats were treated with Zusanli group at the age of 2 months. In Hegu group, the corresponding acupoints were stimulated with electroacupuncture for 20 minutes every time, once every other day for 5 times. The fecal characters of rats before and after electroacupuncture were observed, and the scores of Bristol classification criteria were used. Abdominal withdrawal reflex (AWR) was used to evaluate visceral pain sensitivity. Immunohistochemical method was used to detect the positive expression of 5-HT _ 2AR in colonic capsaicin receptor TRPV1.Results compared with the blank group, the model group before acupuncture. The Bristol scores of Zusanli group and Hegu group were significantly higher than those of Hegu group. After acupuncture, the score of model group was higher than that of Hegu group (P 0.05). In the model group, the ventral pressure threshold of Zusanli group decreased significantly (P 0.01), the contraction wave increased significantly (P 0.01), and the positive expression of TRPV1 in colon increased significantly (P 0.01). Compared with model group, Zusanli group and Hegu group, Zusanli group and Hegu group had higher expression of 5-HT2AR. The score of Bristol stool decreased after acupuncture (P 0.01). The threshold of abdominal lifting pressure increased P0.01a, and the number of contraction waves decreased P0.01a; Compared with Zusanli group, the Bristol score of Hegu group was higher than that of Zusanli group (P 0.05). The expression of TRPV1 in colon decreased P0.01A 5-HT 2AR expression increased. Conclusion electroacupuncture at Zusanli point and Hegu point can reduce the sensitivity of intestinal pain in IBS rats. The therapeutic effect of Hegu point on visceral pain of IBS was slightly better than that of Zusanli point, while Zusanli point had more advantages in regulating gastrointestinal motility. The acupoints innervated by different nerve segments can treat the same disease, but there are differences in the effects, which proves that the effects of the meridians are specific and relative.
【作者單位】： 北京中醫(yī)藥大學(xué);
【基金】：國家自然科學(xué)基金項目(No.81473776)~~
【分類號】：R245;R-332
【正文快照】： 吳艷英,女,在讀碩士生(北京中醫(yī)藥大學(xué)北京100029)tal distension(CRD)test to rats in all groups except the normal control group.These rats were all isolatedfrom their mothers.2-month old rats in the ST36 group and LI14 group received electro-acupuncture five

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