本文關(guān)鍵詞：阿里紅多糖抗氧化和抗腫瘤及免疫調(diào)節(jié)活性的研究　出處：《新疆醫(yī)科大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 阿里紅 多糖 抗氧化 抗腫瘤 免疫調(diào)節(jié)

【摘要】：目的:采用體外細(xì)胞實驗及小鼠模型實驗來研究阿里紅多糖抗氧化、抗腫瘤及免疫調(diào)節(jié)活性,為多糖進(jìn)一步研究提供一定的實驗依據(jù)。方法:1、選擇體外清除自由基反應(yīng)體系,采用二苯代苦味臍基自由基(DPPH·)、鄰苯三酚自氧化法、羥基自由基(·OH)法等方法對考察阿里紅多糖的體外抗氧化能力。2.采用四甲基偶氮哇藍(lán)(MTT)顯色法,觀察不同濃度的阿里紅多糖對鼠源乳腺癌4TI細(xì)胞、人結(jié)腸癌細(xì)胞系HT-29、人宮頸癌細(xì)胞系Hela、人卵巢癌細(xì)胞系SK-OV-3、人前列腺癌細(xì)胞系PC-3、人肝癌細(xì)胞系Hep3B細(xì)胞生長的抑制作用,篩選阿里紅多糖抗腫瘤活性。3.采用ICR小鼠建立4T1荷瘤小鼠模型,觀察阿里紅多糖對模型小鼠移植性腫瘤的抑制作用影響。4.采用RSB法考察對小鼠脾臟T、B淋巴細(xì)胞的影響;探討其體外免疫作用。結(jié)果:1、體外抗氧化結(jié)果顯示阿里紅多糖在體外3種抗氧化體系中均顯示具有抗氧化活性。2、在體外,各劑量濃度的阿里紅多糖對鼠源乳腺癌4Tl細(xì)胞增殖有抑制作用。3、阿里紅多糖能有效抑制實體瘤模型荷瘤小鼠體內(nèi)腫瘤生長,結(jié)果顯示阿里紅多糖對4T1小鼠的抑瘤率分別為58.69%(低劑量組)、60.89%(中劑量組)、74.73%(高劑量組)。4、阿里紅多糖對脾細(xì)胞具有免疫調(diào)節(jié)作用。FoP既能與ConA或LPS協(xié)同誘導(dǎo)小鼠脾T、B淋巴細(xì)胞的增殖,也能直接促進(jìn)小鼠脾T、B淋巴細(xì)胞的增殖;FoP也可提高小鼠脾細(xì)胞CD19+CD86+B細(xì)胞的比值;此外,FoP還可促進(jìn)T淋巴細(xì)胞分泌Th1型細(xì)胞因子IL-2分泌和B細(xì)胞活化和增殖的IL-13的分泌,對主要來源于巨噬細(xì)胞的細(xì)胞因子TNF-α的分泌也有促進(jìn)作用。5、FoP也能誘導(dǎo)小鼠腹腔巨噬細(xì)胞的增殖,能促使來源于巨噬細(xì)胞的細(xì)胞因子TNF-α分泌。結(jié)論:1.阿里紅多糖體外具有抗氧化活性。2.阿里紅多糖在體內(nèi)外具有抑制鼠源乳腺癌細(xì)胞株4T1的細(xì)胞增殖作用。3、能夠顯著促進(jìn)正常小鼠脾淋巴細(xì)胞增殖,且在一定范圍內(nèi)具有劑量依賴關(guān)系,具有免疫作用。
[Abstract]:Objective: to study the anti-oxidation, anti-tumor and immunomodulatory activities of Ari Red polysaccharides in vitro and mouse model experiments, and to provide some experimental basis for further study of polysaccharides. Methods: 1. The free radical scavenging reaction system in vitro was selected and the diphenyl bitter umbilical radical DPPH 路N and pyrogallol autoxidation were used. Hydroxyl radical (路OH) method was used to study the antioxidation ability of Ari Red polysaccharides in vitro. 2. Tetramethylazo blue blue (MTT) method was used. The effects of different concentrations of Ali Red polysaccharides on mouse breast cancer cell line 4TI, human colon cancer cell line HT-29, human cervical cancer cell line Hela and human ovarian cancer cell line SK-OV-3 were observed. The growth inhibition of human prostate cancer cell line PC-3 and human hepatoma cell line Hep3B was studied. The anti-tumor activity of Ali Red polysaccharide was screened. The model of 4T1 tumor-bearing mice was established by using ICR mice. To observe the inhibitory effect of Ali Red Polysaccharide on transplanted tumor in model mice. (4) to investigate the effect of RSB on spleen TnB lymphocytes in mice; Results the results showed that the polysaccharides had antioxidant activity of 0.2 in vitro and showed antioxidant activity in vitro. Different doses of Ali Red polysaccharides can inhibit the proliferation of mouse breast cancer 4TL cells. 3. Ali red polysaccharides can effectively inhibit the tumor growth of solid tumor model mice. The results showed that the inhibitory rates of the polysaccharide against 4T1 mice were 58.69 (low dose group 60.89) (middle dose group 74.73%) (high dose group 74.73%). Ali Red polysaccharides have immunomodulatory effect on spleen cells. Fop can not only induce the proliferation of Thyrosine B lymphocytes in mice spleen in cooperation with ConA or LPS, but also directly promote the proliferation of Thyrosine B lymphocytes in mice spleen. FoP also increased the ratio of CD19 CD86 B cells in spleen cells of mice. In addition, FoP can also promote T lymphocytes to secrete Th1 type cytokine IL-2 secretion and B cell activation and proliferation of IL-13 secretion. It also promoted the secretion of TNF- 偽, a cytokine mainly derived from macrophages, and also induced the proliferation of murine peritoneal macrophages. TNF- 偽, a cytokine derived from macrophages, can be secreted. Conclusion:. 1. Ali red polysaccharide has antioxidant activity in vitro. In vitro and in vivo, Ali red polysaccharide can inhibit the proliferation of mouse breast cancer cell line 4T1. 3. It can significantly promote the proliferation of splenic lymphocytes in normal mice, and has a dose-dependent relationship in a certain range, and has immune effect.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R29

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