本文關(guān)鍵詞：“腎腦相濟(jì)”電針療法對(duì)“天癸竭”圍絕經(jīng)期抑郁癥作用的機(jī)制研究　出處：《遼寧中醫(yī)藥大學(xué)》2016年博士論文　論文類型：學(xué)位論文

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【摘要】：目的:在“腎腦相濟(jì)”理論的指導(dǎo)下,觀察“補(bǔ)腎益精填髓”的電針療法對(duì)“天癸竭”圍絕經(jīng)期抑郁癥的作用,并探討其作用機(jī)制,為臨床治療圍絕經(jīng)期抑郁癥提供指導(dǎo)和實(shí)驗(yàn)依據(jù)。材料與方法:選取17周齡健康雌性SD大鼠120只,隨機(jī)分為正常組、模型組、藥物組、電針組和假手術(shù)組,每組各24只。2只一籠正常飼養(yǎng)。1.行為學(xué)指標(biāo)檢測:各組大鼠在造模開始前先進(jìn)行行為學(xué)指標(biāo)的觀測,行為學(xué)指標(biāo)有曠場實(shí)驗(yàn)中總移動(dòng)距離和曠場中心區(qū)域停留時(shí)間;糖水偏好實(shí)驗(yàn)中糖水消耗量、總液體消耗量和糖水消耗率;強(qiáng)迫游泳實(shí)驗(yàn)中大鼠在水中靜止時(shí)間。2.制備圍絕經(jīng)期抑郁癥模型:正常組大鼠不接受造模,正常飼養(yǎng)。模型組、藥物組和電針組大鼠摘除雙側(cè)卵巢去勢,假手術(shù)組不切除卵巢,只在卵巢附近切除與卵巢等大的脂肪。手術(shù)后大鼠恢復(fù)兩周,之后進(jìn)行連續(xù)5天的陰道涂片,確定手術(shù)組大鼠無動(dòng)情周期。認(rèn)為切除卵巢去勢成功。之后正常組大鼠正常飼養(yǎng)不接受刺激,模型組、藥物組、電針組和假手術(shù)組的大鼠均接受為期21天的慢性不可預(yù)見溫和刺激(CUMS)。刺激包括:冰水游泳、熱水游泳、禁水禁食、夾尾、束縛限制行動(dòng)、傾斜籠子和晝夜顛倒。每種刺激隨機(jī)出現(xiàn),且每種刺激在21天中出現(xiàn)次數(shù)不少于3次。21天刺激結(jié)束后,通過行為學(xué)指標(biāo)的變化確定大鼠圍絕經(jīng)期抑郁癥模型造模是否成功。3.電針治療:正常組大鼠正常喂養(yǎng),不接受治療。電針組和假手術(shù)組的大鼠接受28天的電針治療,根據(jù)“腎腦相濟(jì)”理論,選取百會(huì)、腎俞、三陰交穴,每日電針治療1次,持續(xù)20分鐘。藥物組大鼠選用三環(huán)類抗抑郁藥物鹽酸氯米帕明作為對(duì)照,用鹽酸氯米帕明灌胃,每日灌胃1次。治療結(jié)束后通過行為學(xué)指標(biāo)的變化評(píng)價(jià)電針對(duì)于圍絕經(jīng)期抑郁癥大鼠的治療效果。4.行為學(xué)指標(biāo)觀察結(jié)束后,麻醉處死所有大鼠取材,大鼠大腦石蠟切片用于觀察大鼠海馬結(jié)構(gòu)和神經(jīng)細(xì)胞形態(tài)變化;分離大鼠海馬組織,用酶聯(lián)免疫吸附法檢測海馬組織中腦源性神經(jīng)營養(yǎng)因子(BDNF)、酪氨酸激酶受體B(Trk B)、c AMP反應(yīng)元件結(jié)合蛋白(CREB)、B細(xì)胞淋巴瘤2(Bcl-2)和Bcl-2相關(guān)X蛋白(Bax)的含量,并用實(shí)時(shí)熒光定量PCR的方法檢測海馬組織內(nèi)BDNF、Trk B、CREB、Bcl-2和Bax的m RNA表達(dá)量。結(jié)果:1.造模開始前各組大鼠在曠場實(shí)驗(yàn)中總移動(dòng)距離和中心區(qū)域停留時(shí)間無差異(p0.05)。糖水實(shí)驗(yàn)中,糖水消耗量、總液體消耗量和糖水消耗率無差異(p0.05);強(qiáng)迫游泳實(shí)驗(yàn)中靜止不動(dòng)時(shí)間無差異(p0.05)。2.雙側(cè)卵巢摘除術(shù)后,除正常組和假手術(shù)組外,各組大鼠陰道涂片均無動(dòng)情周期。3.經(jīng)過21天慢性不可預(yù)見溫和刺激后,曠場實(shí)驗(yàn)結(jié)果:與正常組相比,其余各組大鼠的總移動(dòng)距離各組均小于正常組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。其余各組大鼠曠場中心區(qū)域停留時(shí)間各組均小于正常組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。4.經(jīng)過21天慢性不可預(yù)見溫和刺激后,糖水實(shí)驗(yàn)結(jié)果:與正常組比較,其余各組大鼠糖水消耗率均小于正常組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。與模型組比較,假手術(shù)組大鼠糖水消耗率高于模型組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。5.經(jīng)過21天慢性不可預(yù)見溫和刺激后,強(qiáng)迫游泳實(shí)驗(yàn)結(jié)果:與正常組比較,其余各組大鼠水中靜止時(shí)間均大于正常組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。6.經(jīng)過28天的電針和藥物治療后,曠場實(shí)驗(yàn)結(jié)果:與正常組比較,模型組大鼠的總移動(dòng)距離小于正常組,中心區(qū)域停留時(shí)間小于正常組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。與模型組比較,藥物組、電針組和假手術(shù)組的總移動(dòng)距離大于模型組,曠場中心區(qū)域停留時(shí)間大于模型組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。7.經(jīng)過28天的電針和藥物治療后,糖水實(shí)驗(yàn)結(jié)果:與正常組比較,其余各組大鼠糖水消耗率均少于正常組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。與模型組比較,假手術(shù)組、電針組和藥物組的糖水消耗率高于模型組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。與電針組比較,假手術(shù)組糖水消耗率高于電針組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。8.經(jīng)過28天的電針和藥物治療后,強(qiáng)迫游泳實(shí)驗(yàn)結(jié)果:與正常組比較,模型組大鼠靜止時(shí)間大于正常組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。與模型組比較,藥物組、電針組和假手術(shù)組的靜止時(shí)間小于模型組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。9.經(jīng)過28天的電針和藥物治療后,在光鏡下觀察各組大鼠海馬石蠟切片,正常組大鼠海馬CA3區(qū)錐體細(xì)胞層和齒狀回區(qū)顆粒細(xì)胞層細(xì)胞數(shù)量多、排列整齊,神經(jīng)元結(jié)構(gòu)完整,胞核及胞質(zhì)染色均勻、清晰。尼氏染色可見神經(jīng)元胞質(zhì)內(nèi)含有豐富的尼氏體,呈紫藍(lán)色,神經(jīng)元細(xì)胞軸突樹突形態(tài)結(jié)構(gòu)完整。模型組大鼠海馬CA3區(qū)細(xì)胞數(shù)量少,細(xì)胞間隙增大,排列疏松不齊,甚至出現(xiàn)細(xì)胞層中斷,尼氏染色可見神經(jīng)元細(xì)胞核消失,細(xì)胞質(zhì)萎縮、出現(xiàn)空洞,軸突樹突結(jié)構(gòu)不完整,甚至出現(xiàn)細(xì)胞結(jié)構(gòu)消失。藥物組、電針組和假手術(shù)組的情況介于兩者之間。10.經(jīng)過28天的電針和藥物治療后,海馬組織酶聯(lián)免疫吸附法結(jié)果:與正常組相比,模型組大鼠海馬BDNF蛋白含量明顯降低,差異有統(tǒng)計(jì)學(xué)意義(p0.05);與模型組比較,藥物組、電針組和假手術(shù)組均高于模型組,差異有統(tǒng)計(jì)學(xué)意義(p0.05);與電針組比較,假手術(shù)組的數(shù)值高于正常組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。與正常組相比,模型組大鼠海馬Trk B蛋白含量明顯降低,差異有統(tǒng)計(jì)學(xué)意義(p0.05);與模型組比較,藥物組、電針組和假手術(shù)組均高于模型組,差異有統(tǒng)計(jì)學(xué)意義(p0.05);與電針組比較,假手術(shù)組的數(shù)值低于正常組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。與正常組相比,模型組大鼠海馬CREB蛋白含量降低,差異有統(tǒng)計(jì)學(xué)意義(p0.05);與模型組比較,藥物組、電針組和假手術(shù)組含量高于模型組,差異有統(tǒng)計(jì)學(xué)意義(p0.05);與電針組比較,假手術(shù)組含量高于電針組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。與正常組相比,模型組大鼠海馬中Bcl-2蛋白含量低于正常組,Bax含量高于正常組,差異有統(tǒng)計(jì)學(xué)意義(p0.05);與模型組比較,藥物組、電針組和假手術(shù)組Bcl-2含量高于模型組,Bax含量低于模型組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。與正常組比價(jià),模型組大鼠Bcl-2/Bax比例關(guān)系大于正常組,差異有統(tǒng)計(jì)學(xué)意義(p0.05);與模型組比較,藥物組、電針組和假手術(shù)組Bcl-2/Bax比例關(guān)系大于模型組,差異有統(tǒng)計(jì)學(xué)意義(p0.05);與電針組比較,假手術(shù)組Bcl-2/Bax比例關(guān)系大于電針組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。11.經(jīng)過28天的電針和藥物治療后,海馬組織實(shí)時(shí)熒光定量PCR結(jié)果:與正常組相比,模型組大鼠海馬BDNF m RNA相對(duì)表達(dá)量低于正常組,差異有統(tǒng)計(jì)學(xué)意義(p0.05);與模型組比較,藥物組、電針組和假手術(shù)組相對(duì)表達(dá)量高于模型組,差異有統(tǒng)計(jì)學(xué)意義(p0.05);與電針組比較,假手術(shù)組BDNF m RNA相對(duì)表達(dá)量高于電針組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。與正常組相比,模型組大鼠海馬Trk B m RNA相對(duì)表達(dá)量高于正常組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。與正常組相比,模型組大鼠海馬CREB m RNA相對(duì)表達(dá)量低與正常組,差異有統(tǒng)計(jì)學(xué)意義(p0.05);與模型組比較,藥物組、電針組和假手術(shù)組相對(duì)表達(dá)量高于模型組,差異有統(tǒng)計(jì)學(xué)意義(p0.05);與電針組比較,假手術(shù)組CREB m RNA相對(duì)表達(dá)量高于電針組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。與正常組相比,模型組大鼠海馬Bcl-2 m RNA相對(duì)表達(dá)量低于正常組,Bax m RNA相對(duì)表達(dá)量高于正常組,差異有統(tǒng)計(jì)學(xué)意義(p0.05);與模型組比較,藥物組、電針組和假手術(shù)組Bcl-2 m RNA相對(duì)表達(dá)量高于模型組,Bax m RNA相對(duì)表達(dá)量低于模型組,差異有統(tǒng)計(jì)學(xué)意義(p0.05);與電針組比較,假手術(shù)組Bcl-2 m RNA相對(duì)表達(dá)量高于電針組,Bax m RNA相對(duì)表達(dá)量低于電針組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。結(jié)論:1.去勢造模結(jié)合慢性不可預(yù)見溫和刺激確立圍絕經(jīng)期抑郁癥大鼠模型。2.基于“腎腦相濟(jì)”理論的電針治療能改善圍絕經(jīng)期大鼠的抑郁癥狀,實(shí)驗(yàn)結(jié)果與臨床吻合,且為臨床提供實(shí)驗(yàn)依據(jù)。3.假手術(shù)組和電針組行為學(xué)指標(biāo)結(jié)果略有不同,說明“天癸竭”在圍絕經(jīng)期抑郁癥的發(fā)病和轉(zhuǎn)歸中有著重要作用。4.圍絕經(jīng)期抑郁癥大鼠“髓海空虛”的生物學(xué)基礎(chǔ)可能是大腦海馬神經(jīng)元細(xì)胞數(shù)量減少和細(xì)胞結(jié)構(gòu)破壞,且與海馬神經(jīng)元細(xì)胞凋亡增加、海馬神經(jīng)發(fā)生降低有關(guān)。5.電針能高提高大鼠海馬內(nèi)BDNF、Trk B和CREB的含量,促進(jìn)海馬神經(jīng)發(fā)生,還能提高Bcl-2和Bax的比例,抑制神經(jīng)元細(xì)胞凋亡,電針“補(bǔ)腎益精填髓”的作用是通過促進(jìn)神經(jīng)發(fā)生和抑制細(xì)胞凋亡實(shí)現(xiàn)的。6.模型組大鼠海馬組織中Trk B m RNA相對(duì)表達(dá)量高但Trk B蛋白含量低,說明圍絕經(jīng)期抑郁癥大鼠體內(nèi)某些因素妨礙了Trk B基因的表達(dá),這可能是慢性不可預(yù)見溫和刺激造成海馬中Trk B和BDNF含量減少的原因。
[Abstract]:Objective: in the kidney and brain of mercy "under the guidance of the theory, to observe the effect of" kidney tonifying the kidney essence of electro acupuncture therapy on the "Tiangui dried up" perimenopausal depression, and to explore its mechanism, provide guidance and experimental basis for the clinical treatment of perimenopausal depression. Materials and methods: 17 week old healthy 120 female SD rats were randomly divided into normal group, model group, drug group, EA group and sham group, 24 rats in each group.2 only a normal feeding behavior of.1. cage indexes: the rats in the observation before the start of the model of behavioral indicators, behavioral indicators of total mobile field test in the distance and open field center area of residence time; consumption of sugar sucrose preference test, total fluid consumption and sugar consumption rate; forced swimming test in rats in static time.2. preparation model of perimenopausal depression: normal rats do not accept made Die, normal feeding. Model group, drug group and EA group rats were bilaterally ovariectomized and sham operation group without excision of ovarian resection, only in ovarian and ovarian size near fat. Postoperative recovery of the rats for two weeks, after 5 consecutive days of vaginal smear, determine the operation group rats had no estrous cycle that success. After resection of ovariectomized rats in normal group fed normally does not accept the stimulus, model group, drug group, EA group and sham operation group rats were treated for 21 days chronic unpredictable mild stimulation (CUMS) stimulation. Including: swimming, water swimming, fasting water fasting, tail clamp restrict action, tilt, cage and night. Each stimulus appears randomly, and each stimulus in 21 days is not less than 3 times.21 days after stimulation, changes of indexes through the behavior of the rat model of perimenopausal depression determine the success of modeling.3. Acupuncture treatment: the normal rats were fed normally, do not receive treatment. EA group and sham operation group rats received acupuncture treatment for 28 days, according to the "kidney brain Jinan" theory, select Baihui, Shenshu, Sanyinjiao, acupuncture treatment 1 times daily, for 20 minutes. Rats in drug group with tricyclic antidepressant clomipramine hydrochloride as control with clomipramine hydrochloride intragastric gavage 1 times. To evaluate the changes of electroacupuncture after the end of treatment by behavioral indicators for treatment of perimenopausal depression rats.4. behavior indexes were observed after anesthesia, all the rats were killed at the rat brain, paraffin sections for observation the structure and morphology of hippocampal nerve cell changes; isolated rat hippocampus, adsorption method for detection of hippocampal brain-derived neurotrophic factor by enzyme linked immunosorbent assay (BDNF), tyrosine kinase receptor B (Trk B C), AMP response element binding protein White (CREB), B cell lymphoma 2 (Bcl-2) and Bcl-2 associated X protein (Bax) content, and hippocampuswere measured by real-time quantitative PCR BDNF, Trk B, CREB, m RNA and Bax Bcl-2 expression. Results: 1. before the start of the model rats in the open field the total moving distance and center area of residence time had no difference (P0.05). In the experiment, sugar consumption, total liquid consumption and sugar consumption rate had no difference (P0.05); forced swimming test in stationary time difference (P0.05).2. bilateral ovariectomy, except normal group and sham operation group, the rats had no vaginal smears of the estrous cycle of.3. after 21 days chronic unpredictable mild stimulation, open field test results: compared with normal group, the total moving distance of each group of rats were less than the normal group, the difference was statistically significant (P0.05). The rats in open field center stay The time was shorter than the normal group, the difference was statistically significant (P0.05.4.) after 21 days chronic unpredictable mild stimulation, sucrose test results: compared with normal group, the rats sugar consumption rate is less than the normal group, the difference was statistically significant (P0.05). Compared with the model group, the sham operation group sucrose consumption rate is higher than the model group, the difference was statistically significant (P0.05.5.) after 21 days chronic unpredictable mild stimulation, forced swimming test results: compared with the normal group, the rats in the rest time were higher than the normal group, the difference was statistically significant (P0.05.6.) after 28 days of acupuncture and drugs after treatment, the experimental results of open field: compared with the normal group, the total moving distance of the rats in the model group than the normal group, the center area of the residence time is less than the normal group, the difference was statistically significant (P0.05). Compared with the model group, drug group and EA group The sham operation group and the total moving distance is greater than the model group, the open field center area of the residence time was more than the model group, the difference was statistically significant (P0.05.7.) after electroacupuncture and medication 28 days after treatment, in the experimental results: compared with the normal group, the rats were less than the normal rate of sugar consumption group, was statistically significant the difference (P0.05). Compared with the model group, sham operation group, electroacupuncture group and drug group sugar consumption rate is higher than the model group, the difference was statistically significant (P0.05). Compared with the electroacupuncture group, sham operation group, sugar consumption rate is higher than the acupuncture group, the difference was statistically significant (P0.05.8.) after electroacupuncture and drug 28 days after treatment, forced swimming test results: compared with the normal group, the rest time of rats in the model group than the normal group, the difference was statistically significant (P0.05). Compared with the model group, drug group, EA group and sham operation group the rest time less than model group, The difference was statistically significant (P0.05.9.) after electroacupuncture and medication after 28 days of treatment, observed in the paraffin sections of rats under light microscope, normal rat hippocampal CA3 pyramidal cell layer and dentate gyrus granular cell layer number of cells, rows, neuron structure is complete, the nucleus and the cytoplasm uniform staining and Nissl staining. Clear neuronal cytoplasm contained abundant Nissl bodies, purple blue, the morphology of neurons axon dendritic cells. The number of cells in hippocampal CA3 region of rats in the model group, the cell gap increases, loose arrangement is uneven, and even cell layer disruption, Nissl staining of neuronal nuclei disappeared, cytoplasm atrophy appeared empty, axon dendritic structure is not complete, or even disappear. The cell structure between EA group and drug group, between two sham operation group.10. after electric acupuncture and drug treatment after 28 days of sea The horse tissue ELISA results: compared with normal group, the content of BDNF protein in hippocampus of rats in the model group decreased significantly, the difference was statistically significant (P0.05); compared with the model group, drug group, EA group and sham operation group were higher than those in the model group, the difference was statistically significant (P0.05); compared with the electroacupuncture group numerical, sham operation group was higher than the normal group, the difference was statistically significant (P0.05). Compared with the normal group, model group of rat hippocampal Trk B protein content decreased significantly, the difference was statistically significant (P0.05); compared with the model group, drug group, electric acupuncture group and sham operation group were higher than those in the model group, there are statistically significant difference (P0.05); compared with the electroacupuncture group, sham operation group, the value is less than the normal group, the difference was statistically significant (P0.05). Compared with the normal group, decrease the content of CREB protein in hippocampus of rats in the model group, the difference was statistically significant (P0.05); compared with the model group, drug group, EA group and sham operation group were higher than those in the model group, the difference was statistically significant (P0.05); compared with the electroacupuncture group, sham operation group was higher than that in the EA group, the difference was statistically significant (P0.05). Compared with the normal group, the content of Bcl-2 protein in hippocampus of rats in model group were lower than the normal group, the content of Bax was higher than that of normal group the difference was statistically significant (P0.05); compared with the model group, drug group, EA group and sham operation group Bcl-2 was higher than that in the model group, the Bax content is lower than the model group, the difference was statistically significant (P0.05). Comparison with the normal group, the ratio of Bcl-2/Bax in model group rats than the normal group, the difference was statistically significant (P0.05); compared with the model group, drug group, EA group and sham operation group Bcl-2/Bax proportion is greater than the model group, the difference was statistically significant (P0.05); compared with the electroacupuncture group, sham operation group Bcl-2/ Bax proportion is greater than the acupuncture group, there was statistically significant difference The meaning of (P0.05).11. after electroacupuncture and medication after 28 days of treatment, the result of real-time fluorescence quantitative PCR in hippocampus: compared with normal group, RNA m BDNF in hippocampus of rats in the model group the relative expression amount is less than the normal group, the difference was statistically significant (P0.05); compared with the model group, drug group, EA group and sham operation group the relative expression quantity is higher than the model group, the difference was statistically significant (P0.05); compared with the electroacupuncture group, sham operation group BDNF m relative expression of RNA was higher than the acupuncture group, the difference was statistically significant (P0.05). Compared with the normal group, RNA B m Trk in hippocampus of rats in the model group the relative expression quantity is higher than the normal group, there are statistically significant difference (P0.05). Compared with the normal group, the RNA m CREB in hippocampus of rats in the model group is relatively low expression with the normal group, the difference was statistically significant (P0.05); compared with the model group, drug group, EA group and sham operation group relative expression quantity is higher than the model group, the difference was statistically Statistically significant (P0.05); compared with the electroacupuncture group, sham operation group CREB m relative expression of RNA was higher than the acupuncture group, the difference was statistically significant (P0.05). Compared with the normal group, the RNA m Bcl-2 in hippocampus of rats in the model group the relative expression of Bax m were lower than the normal group, the relative expression level of RNA is higher than the normal group, there are statistically significant difference (P0.05); compared with the model group, drug group, EA group and sham operation group Bcl-2 m RNA relative expression quantity is higher than the model group, the relative expression of RNA Bax m lower than that of model group, the difference was statistically significant (P0.05); compared with the electroacupuncture group, sham operation group Bcl-2 m relative expression of RNA was higher than that of EA group, Bax m RNA relative expression is lower than the acupuncture group, the difference was statistically significant (P0.05). Conclusion: 1. ovariectomized model combined with chronic unpredictable mild stimulation to establish perimenopausal depression rat model of.2. based on the "kidney brain mercy" theory of electroacupuncture therapy can improve perimenopausal The menstrual period in rats with depressive symptoms, are in good agreement with the experimental results and clinical, and provide experimental basis for clinical.3. sham operation group and electroacupuncture group behavioral indexes were slightly different, "Tiangui dried up" at the onset of perimenopausal depression and prognosis has important biological basis of the role of.4. in perimenopausal depression rats' spinal cord the sea empty "may be the number of cells in hippocampal neurons of brain damage and reduce the cell structure, and increase and apoptosis of hippocampal neurons, hippocampal neurogenesis is related to the decrease of.5. EA can improve the high in rat hippocampus BDNF, Trk content B and CREB, to promote neurogenesis in the hippocampus, Bcl-2 and Bax can improve the proportion of inhibitory neurons cell apoptosis, effect of acupuncture of" kidney tonifying the kidney essence "is through the promotion of high volume but Trk B m RNA Tr relative expression of neurogenesis and cell proliferation and apoptosis.6. in hippocampus of rats in model group K B protein content is low, indicating that some factors in the perimenopausal depression rats interfere with the expression of Trk B gene, which may be the reason why the Trk B and BDNF contents in hippocampus are reduced due to chronic unpredictable mild stimulation.

【學(xué)位授予單位】：遼寧中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2016
【分類號(hào)】：R245

【參考文獻(xiàn)】

相關(guān)期刊論文 前5條

1 楊蘭;劉保庚;張培浩;楊明末;;調(diào)沖解郁湯治療圍絕經(jīng)期抑郁癥60例臨床觀察[J];中國實(shí)驗(yàn)方劑學(xué)雜志;2015年12期

2 孟安琪;高超;侯小艷;李默;孫劍端;任路;;補(bǔ)腎療法治療圍絕經(jīng)期抑郁癥治驗(yàn)[J];遼寧中醫(yī)雜志;2013年09期

3 湯翔群;關(guān)紅;;圍絕經(jīng)期抑郁癥婦女FSH、LH、E2激素水平分析[J];實(shí)驗(yàn)與檢驗(yàn)醫(yī)學(xué);2008年06期

4 何志暉;張曉薇;麥小玲;藍(lán)文莉;王子蓮;;圍絕經(jīng)期婦女抑郁癥相關(guān)因素及預(yù)防策略[J];中國實(shí)用婦科與產(chǎn)科雜志;2007年12期

5 左玲俊,徐俊冕;HPA軸功能與抑郁癥[J];中國心理衛(wèi)生雜志;2001年02期

相關(guān)博士學(xué)位論文 前1條

1 宋映周;針刺對(duì)CUMS模型大鼠ERK信號(hào)轉(zhuǎn)導(dǎo)通路作用機(jī)制研究[D];北京中醫(yī)藥大學(xué);2015年

，

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