本文關(guān)鍵詞：基于“耳鼻喉科脾胃論”應(yīng)用補(bǔ)中益氣顆粒對(duì)脾氣虛型變應(yīng)性鼻炎大鼠血清IL-3的影響及臨床療效研究　出處：《安徽中醫(yī)藥大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 變應(yīng)性鼻炎 脾氣虛型 補(bǔ)中益氣顆粒 療效觀察 IL-3

【摘要】：1目的臨床與實(shí)驗(yàn)共同觀察補(bǔ)中益氣顆粒是否能成為治療AR更有效、更安全的藥物,探索并總結(jié)補(bǔ)中益氣顆粒治療脾氣虛型AR的理論基礎(chǔ)。2方法2.1臨床研究所有病例均來源于2015年3月~2015年10月安徽中醫(yī)藥大學(xué)第一附屬醫(yī)院耳鼻喉科門診,選取其中60例符合標(biāo)準(zhǔn)的脾氣虛型AR患者,隨機(jī)分為補(bǔ)中益氣顆粒組(治療組)30例及依巴斯汀組(對(duì)照組)30例,2周為1療程,分別觀察并記錄治療過程中兩組鼻部癥狀、體征及全身癥狀,分別在治療前、治療1周、治療2周及隨訪1周的變化,并留意是否有不良反應(yīng)出現(xiàn),對(duì)所得數(shù)據(jù)進(jìn)行統(tǒng)計(jì)學(xué)分析。2.2實(shí)驗(yàn)研究選取48只健康SD大鼠,適應(yīng)性地飼養(yǎng)2~3天后,隨機(jī)將它們分為正常對(duì)照組12只、AR模型組各12只、脾氣虛模型組24只;然后脾氣虛模型組經(jīng)評(píng)價(jià)造模成功后,再次分為脾氣虛AR模型組、脾氣虛AR中藥模型組,每組12只。采用雙抗夾心ELISA法,橫坐標(biāo)為標(biāo)準(zhǔn)品濃度,縱坐標(biāo)為OD值,繪制標(biāo)準(zhǔn)曲線,并根據(jù)血清樣品的OD值可在標(biāo)準(zhǔn)曲線上查出其濃度,可以檢測(cè)出不同分組的大鼠血清IL-3濃度,再使用SPSS17.0軟件包,對(duì)所得數(shù)據(jù)進(jìn)行對(duì)比分析。3結(jié)果3.1臨床觀察結(jié)果3.1.1治療1周后,治療組的有效率卻為9.3%,對(duì)照組的有效率21.2%。3.1.2治療2周后,治療組的有效率為23.6%,而對(duì)照組的有效率29.8%。3.1.3隨訪1周后,治療組優(yōu)于對(duì)照組,有效率分別為35.5%、16.9%。3.1.4治療1周后,經(jīng)Independent-Samples Tests檢驗(yàn),得出流涕、鼻甲腫大、食少納呆、少氣懶言和大便溏稀積分P0.05,有統(tǒng)計(jì)學(xué)意義,說明兩組治療1周后,在改善流涕、鼻甲腫大、食少納呆、少氣懶言和大便溏稀癥狀方面,對(duì)照組療效優(yōu)于治療組。3.1.5治療2周后,經(jīng)Independent-Samples Tests檢驗(yàn),得出噴嚏和食少納呆積分P0.05,有統(tǒng)計(jì)學(xué)意義,說明治療2周后,對(duì)照組在改善噴嚏和食少納呆癥狀方面,其療效優(yōu)于治療組。3.1.6隨訪1周后,經(jīng)Independent-Samples Tests檢驗(yàn),得出噴嚏、鼻癢和食后腹脹積分P0.05,差異具有統(tǒng)計(jì)學(xué)意義,說明兩組在隨訪1周后,在改善噴嚏、鼻癢和食后腹脹癥狀方面,治療組療效優(yōu)于對(duì)照組。3.2實(shí)驗(yàn)研究結(jié)果使用SPSS17.0軟件,應(yīng)用Independent-Samples Tests方法得出以下結(jié)論。3.2.1正常組與AR組對(duì)比,得出P=0.028,P0.05,有統(tǒng)計(jì)學(xué)意義,由于IL-3濃度(SX±)AR組30.984±22.836大于正常組13.224±12.560,說明AR組大鼠血清IL-3濃度異常,表達(dá)過量;3.2.2正常組與脾氣虛型AR組對(duì)比,得出P=0.003,P0.05,有統(tǒng)計(jì)學(xué)意義,由于IL-3濃度(SX?)脾氣虛型AR組44.803±30.662大于正常組13.224±12.560,說明脾氣虛型AR組大鼠血清IL-3濃度異常,表達(dá)過量;3.2.3正常組與脾氣虛行AR中藥組對(duì)比,得出P=0.040,P0.05,有統(tǒng)計(jì)學(xué)意義,由于IL-3濃度(SX?)脾氣虛型AR中藥組30.075±23.526大于正常組13.224±12.560,說明脾氣虛型AR中藥組大鼠血清IL-3濃度異常,表達(dá)過量。3.2.4觀察分析濃度(SX?)數(shù)值大小,可以看出正常組大鼠血清IL-3濃度含量明顯低于其他三組,脾氣虛型AR組濃度最高,脾氣虛型AR中藥組是脾氣虛型AR組通過服用補(bǔ)中益氣顆粒后造模獲得,而脾氣虛型AR中藥組濃度卻低于AR組和脾氣虛型AR組,考慮脾氣虛型AR組濃度在服用補(bǔ)中益氣顆粒后明顯降低,接近并低于AR組濃度,向正常組恢復(fù)。4結(jié)論4.1臨床研究結(jié)論4.1.1治療1周后,在改善流涕、鼻甲腫大、食少納呆、少氣懶言和大便溏稀癥狀方面,對(duì)照組療效優(yōu)于治療組。4.1.2治療2周后,對(duì)照組在改善噴嚏和食少納呆癥狀方面,對(duì)照組療效優(yōu)于治療組。4.1.3隨訪1周后,在改善噴嚏、鼻癢和食后腹脹癥狀方面,治療組療效優(yōu)于對(duì)照組。4.1.4隨訪2周后,對(duì)照組治療AR的遠(yuǎn)期療效不理想,病情易反復(fù),癥狀易復(fù)發(fā),而治療組反復(fù)率低,遠(yuǎn)期療效可觀,療效具有持續(xù)性、穩(wěn)定性及長(zhǎng)遠(yuǎn)性,臨床更具有推廣價(jià)值。4.2實(shí)驗(yàn)研究結(jié)論4.2.1造模成功后,大鼠血清IL-3的濃度不同。4.2.2 AR模型組的大鼠血清內(nèi)可檢測(cè)到IL-3濃度明顯升高,脾氣虛AR組IL-3的濃度相較于AR組進(jìn)一步升高。4.2.3脾氣虛AR中藥組,IL-3的濃度較高,但相較于脾氣虛AR組是有明顯下降趨勢(shì)的,同時(shí)也低于AR組。4.2.4應(yīng)用補(bǔ)中益氣顆粒后,可以調(diào)節(jié)脾氣虛AR大鼠血清IL-3的濃度,從而達(dá)到治療脾氣虛AR的目的,為補(bǔ)中益氣顆粒治療脾氣虛AR提供了實(shí)驗(yàn)依據(jù)。
[Abstract]:1 the purpose of clinical and experimental observations of Buzhong Yiqi granules can become a more effective treatment of AR, drug safety, and explore the theoretical basis of.2 methods of Buzhong Yiqi granule in treatment of spleen deficiency type AR 2.1 clinical study of all cases were from the First Affiliated Hospital in March 2015 October ~2015 Anhui University of traditional Chinese medicine outpatient department of ENT, select one of the 60 patients with spleen deficiency type AR patients were randomly divided into group of Buzhong Yiqi granules (treatment group) 30 cases and ebastine group (control group) 30 cases, 2 weeks as 1 course of treatment, were observed and recorded in two groups during the treatment of nasal symptoms, signs and symptoms, respectively before treatment, treatment 1 week, change for 2 weeks and 1 weeks follow-up, and pay attention to whether the adverse reactions were statistically analyzed, experimental study of.2.2 selected 48 healthy SD rats of the data, adaptive feeding for 2 ~3 days, it will be random They are divided into 12 rats in normal control group, AR model group with 12 rats in each group, spleen deficiency model group 24 and model group; spleen deficiency by evaluation after the success of the model, again divided into AR model group of spleen deficiency, spleen deficiency AR Chinese medicine model group, 12 rats in each group. With the method of double antibody ELISA abscissa as the standard concentration, the vertical axis is OD, drawing the standard curve, and according to the OD value of serum samples can be found in its concentration on the standard curve, can detect the serum IL-3 concentration of different groups, and then use the SPSS17.0 software package, the analysis of the data of.3 between the results of the 3.1 clinical observation results of 3.1.1 after 1 weeks of treatment and the effective rate of treatment group is 9.3%, the effective rate in the control group 21.2%.3.1.2 after 2 weeks of treatment, the treatment group was 23.6%, while the control group 29.8%.3.1.3 after 1 weeks of follow-up, the treatment group than the control group, respectively 35.5%, 16.9%.3.1.4 after 1 weeks of treatment by I Ndependent-Samples Tests test, the runny nose, nasal swelling, anorexia, less gas lazy words and loose stool score P0.05 was statistically significant, indicating that the two groups after 1 weeks of treatment, in improving runny nose, turbinate swelling, anorexia, less gas lazy words and loose stool symptoms after 2 weeks, the control group efficacy of the treatment group than.3.1.5 treatment by Independent-Samples Tests test, the sneezing and anorexia integral P0.05, have statistical significance, that after 2 weeks of treatment, the control group in improving the symptoms of sneezing and anorexia, its curative effect is better than the treatment group.3.1.6 after 1 weeks of follow-up, the Independent-Samples Tests test, that sneezing, nasal itching after eating and distension P0.05, the difference was statistically significant, indicating that the two groups in the follow-up of 1 weeks, in improving sneezing, nasal itching and hand bloating after eating symptoms, the treatment group than the control group.3.2 experimental results using SPSS17.0 The application of Independent-Samples Tests software, obtained the following conclusions compared to normal.3.2.1 group and AR group reached P=0.028, P0.05, have statistical significance, due to the concentration of IL-3 (SX + AR) = 30.984 + 22.836 + 12.560 13.224 higher than the normal group, the serum IL-3 concentration of rats in group AR were abnormal, excessive expression of 3.2.2 in normal group and temper; the type of deficiency of AR group contrast, obtained P=0.003, P0.05, have statistical significance, due to the concentration of IL-3 (SX?) and spleen deficiency AR group 44.803 + 30.662 + 12.560 13.224 higher than the normal group, serum IL-3 of AR rats and spleen deficiency were abnormal, excessive expression of 3.2.3; the normal group and the spleen deficiency AR group contrast of traditional Chinese medicine come, P=0.040, P0.05, have statistical significance, due to the concentration of IL-3 (SX?) and spleen deficiency AR traditional Chinese medicine group 30.075 + 13.224 + 12.560 23.526 more than the normal group, serum and spleen deficiency AR rats IL-3 abnormal expression of excess.3.2.4 concentration, concentration observation and analysis Degree (SX?) value, can be seen in normal rats serum IL-3 concentration was significantly lower than that of the other three groups, spleen deficiency AR group was the highest, AR traditional Chinese medicine group is spleen qi deficiency and spleen deficiency AR group by taking Buzhong Yiqi granules after modeling, and the spleen deficiency type AR is lower than the concentration of Chinese medicine group AR group and AR group and spleen deficiency, spleen deficiency AR group considered concentration decreased significantly in the use of Buzhong Yiqi granules, close and lower concentration of AR groups, the normal group to restore conclusion conclusion 4.1.4 clinical study of 4.1.1 after 1 weeks of treatment, in improving runny nose, turbinate swelling, anorexia, less gas lazy words and loose stool symptoms after 2 weeks, the control group curative effect is better than that of.4.1.2 in the treatment group, the control group in improving the symptoms of sneezing and anorexia, the control group is better than the treatment group.4.1.3 after 1 weeks of follow-up, in improving sneezing, nasal itching and hand bloating after eating symptoms, the curative effect of treatment group was superior In the control group.4.1.4 after 2 weeks of follow-up, the control group curative effect in the treatment of AR is not ideal, easy to relapse, recurrence symptoms, while the treatment group repeated rate is low, long-term effect is considerable, the effect is persistent, and long-term stability, has more clinical value of.4.2 conclusion the experimental study of 4.2.1 after successful modeling, large the serum IL-3 concentration of different.4.2.2 AR model group rat serum can detect IL-3 concentration obviously, concentration of spleen deficiency AR group IL-3 compared with group AR further increased.4.2.3 of spleen deficiency AR traditional Chinese medicine group, the concentration of IL-3 is higher, but compared to the spleen deficiency AR group is significantly decreased, at the same time lower than the AR group.4.2.4 application of Buzhong Yiqi granules can regulate the serum IL-3 concentration after AR rat spleen deficiency, so as to achieve the treatment of spleen deficiency AR to provide experimental basis for Buzhong Yiqi granule in treatment of spleen deficiency AR.

【學(xué)位授予單位】：安徽中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R276.1

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5 李娟娟;固脫法對(duì)脾氣虛型脫垂痔伴便秘的臨床研究[D];北京中醫(yī)藥大學(xué);2016年

6 王皖湘;基于“耳鼻喉科脾胃論”應(yīng)用補(bǔ)中益氣顆粒對(duì)脾氣虛型變應(yīng)性鼻炎大鼠血清IL-3的影響及臨床療效研究[D];安徽中醫(yī)藥大學(xué);2016年

7 練穎;黃芪治療氣虛型慢性特發(fā)性血小板減少性紫癜的臨床研究[D];成都中醫(yī)藥大學(xué);2001年

8 劉穎;脾氣虛型多囊卵巢綜合征的臨證特點(diǎn)研究[D];復(fù)旦大學(xué);2009年

9 張力文;慢性阻塞性肺疾病中醫(yī)氣虛證候研究[D];北京中醫(yī)藥大學(xué);2005年

10 魏洪玉;中醫(yī)卒中單元下的健康教育對(duì)氣虛型中風(fēng)病人的影響[D];山東中醫(yī)藥大學(xué);2009年

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