本文關(guān)鍵詞：廣西民族藥材走馬胎、血黨質(zhì)量標(biāo)準(zhǔn)實驗研究　出處：《廣西中醫(yī)藥大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 走馬胎 血黨 沒食子酸 質(zhì)量標(biāo)準(zhǔn) 指紋圖譜

【摘要】：目的:通過實驗研究廣西民族藥材走馬胎、血黨的質(zhì)量標(biāo)準(zhǔn),初步擬定走馬胎和血黨的質(zhì)量標(biāo)準(zhǔn)及建立走馬胎、血黨的HPLC指紋圖譜研究方法,為藥材的質(zhì)量控制提供可靠的依據(jù)。方法:(1)參照《中國藥典》2015年版通則對走馬胎和血黨藥材進行水分、總灰分、浸出物的測定。(2)運用微波消解ICP-MS檢測走馬胎和血黨藥材中重金屬Cu、As、Hg、Cd、Pb的含量。(3)以沒食子酸為對照品,參照藥典的方法進行TLC鑒別。(4)運用HPLC,測定廣西不同產(chǎn)地和采集時間的走馬胎和血黨藥材中沒食子酸的含量。(5)運用HPLC,通過相似度軟件對不同產(chǎn)地的藥材進行分析,得到指紋圖譜的共有模式。結(jié)果:(1)走馬胎藥材中水分含量5.65%~12.77%;總灰分含量1.54%~2.63%;酸不溶性灰分含量0.07%~0.62%;浸出物含量10.14%~17.36%。血黨藥材水分含量10.69%~12.50%;總灰分含量4.07%~4.85%;酸不溶性灰分含量0.32%~1.05%;浸出物含量12.03%~17.94%。(2)走馬胎藥材中重金屬Cu、As、Hg、Pb的含量均符合標(biāo)準(zhǔn)規(guī)定;Cd的含量除2013年7月廣西來賓市金秀縣采集的走馬胎藥材外,其余批次均符合規(guī)定。10批血黨藥材中重金屬Cu、Cd、Hg均符合藥典標(biāo)準(zhǔn)規(guī)定,其中有2批血黨藥材中As的含量超過標(biāo)準(zhǔn)規(guī)定,4批血黨藥材中Pb的含量不符合標(biāo)準(zhǔn)。(3)走馬胎和血黨藥材的TLC鑒別方法可靠且重復(fù)性好。得到清晰的斑點,分離效果好。(4)HPLC法測定沒食子酸的含量,經(jīng)方法學(xué)驗證,具有良好的線性關(guān)系、精密度、重復(fù)性和穩(wěn)定性,加樣回收率均在95%-105%范圍內(nèi)。(5)不同批次的走馬胎和血黨藥材指紋圖譜相似度結(jié)果均大于0.9,各特征峰分離度較好,符合指紋圖譜檢測要求。結(jié)論:綜合上述的實驗結(jié)果,初步建立了走馬胎和血黨藥材的質(zhì)量標(biāo)準(zhǔn),為走馬胎和血黨藥材的評價和分析提供了較科學(xué)的實驗依據(jù)。初步建立走馬胎和血黨藥材HPLC指紋圖譜的研究方法,該方法具有準(zhǔn)確性、可靠性、特征性強的特點,穩(wěn)定性、精密度和重復(fù)性結(jié)果均符合要求,可廣泛運用于走馬胎和血黨藥材的質(zhì)量評價。
[Abstract]:Objective: to study the quality standard of horse fetus and blood party of Guangxi nationality medicinal materials, to draw up the quality standard of walking horse fetus and blood party and to establish the HPLC fingerprint study method of walking horse fetus and blood party. Methods according to the general rules of the 2015 edition of Chinese Pharmacopoeia, the moisture content and total ash content of the Chinese medicinal materials were determined. Determination of the extract by microwave digestion ICP-MS method for the determination of the content of heavy metal CuAsAsHgfen CDPb. 3) Gallic acid was used as the reference substance. According to the method of Pharmacopoeia, TLC was used to identify the content of Gallic acid in different producing areas and collecting time in Guangxi. The content of Gallic acid was determined by HPLC. The common pattern of fingerprint was obtained by using similarity software. Results: the water content in the crude drug was 5.65 ~ 12.77% and the content of water was 5.65 ~ (12.77). The total ash content was 1.54 and 2.63. The content of acid insoluble ash was 0.07% and 0.62%; The content of the extract was 10.14% and 17.36%. The water content of Xue Dang medicinal material was 10.699.12.50%. The total ash content is 4.07 and 4.85; The acid insoluble ash content was 0.32% and 1.05%; The content of the heavy metal Cu _ (AsA) H _ (+) H _ (G) Pb in the extract 12.03 ~ 17.94 ~ (2) is in accordance with the standard. In addition to the contents of CD collected in July 2013 in Jinxiu County of Laibin City, Guangxi, the other batches were in accordance with the requirements of the Pharmacopoeia standard for the heavy metal Cu ~ (2 +) CD ~ (2 +) Hg in 10 batches of Xue Dang medicinal materials. Among them, 2 batches of as in Xue Dang medicinal materials exceeded the standard. The content of Pb in 4 batches of Xue Dang medicinal materials was not up to the standard. 3) the TLC identification method of the Hedyotis and Xue Dang herbs was reliable and reproducible, and the clear spots were obtained. The content of Gallic acid was determined by HPLC. The method was proved to be linear, precise, reproducible and stable. The recoveries were in the range of 95 ~ 105%.) the similarity between the fingerprint of different batches of walking horse fetus and Xue Dang was greater than 0.9, and the separation degree of each characteristic peak was better. Conclusion: based on the above experimental results, the quality standard of the crude drugs was established preliminarily. It provides a scientific experimental basis for the evaluation and analysis of walking horse fetus and Xueduan medicinal materials. A preliminary study method of HPLC fingerprint of walking horse fetus and Xue Dou medicinal materials is established. The method is accurate and reliable. The characteristic characteristics, stability, precision and reproducibility all meet the requirements, and can be widely used to evaluate the quality of the foetus and Xue Dang medicinal materials.
【學(xué)位授予單位】：廣西中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R29

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