本文關(guān)鍵詞：基于“健脾益氣”法探討針刺脾俞穴聯(lián)合人參皂苷Rg3抗疲勞機制的實驗研究　出處：《遼寧中醫(yī)藥大學》2016年博士論文　論文類型：學位論文

  更多相關(guān)文章： 慢性疲勞綜合征 細胞免疫 人參皂苷Rg3 針刺 脾俞穴

【摘要】：目的:本課題采用高效液相色譜法觀察針刺“脾俞”穴對人參皂苷Rg3在慢性疲勞模型大鼠不同時相臟腑的分布代謝影響,采用光學顯微鏡觀察大鼠血清中T淋巴細胞轉(zhuǎn)化率,流式細胞術(shù)檢測大鼠脾臟中T細胞亞群CD_3~+T細胞、CD_4~+T細胞及CD_8~+T細胞比例,CD_4~+/CD_8~+的比值,ELISA法檢測血清中IFN-γ和IL-4含量變化,RT-PCR法檢測脾臟中IFN-γ和IL-4 m RNA表達水平,采用Western Blot法檢測脾臟中IFN-γ和IL-4蛋白表達水平,探討針刺“脾俞”穴聯(lián)合人參皂苷Rg3,“針藥結(jié)合”益氣健脾抗疲勞的作用機理。材料與方法:論文一:雄性Wistar大鼠116只隨機分為4組:空白對照組(33只),模型組(33只),空白加針刺組(25只),模型加針刺組(25只)。采用強制冷水游泳結(jié)合慢性束縛法進行模型復制。造模時間21d。21d后,模型組和空白對照組大鼠各隨機抽取8只大鼠,通過檢測其在造模前后的體重和血清乳酸含量的改變情況來判定造模是否成功。空白加針刺組和模型加針刺組于模型復制成功后,給予針刺“脾俞”穴治療,進針后針柄接電針治療儀,電壓4.5 V,頻率為4/20 Hz頻率的疏密波,強度以大鼠局部皮膚肌肉微顫為度,留針20 min,1次/d,第7天針刺結(jié)束后,各組大鼠立即尾靜脈注射人參皂苷Rg3。各組大鼠分別取5只,于給藥后5、30、60、120 min經(jīng)大鼠眼眶取血2ml于肝素化離心管中,3000 rpm離心10 min,分離血漿,高效液相色譜法檢測各時間段各組大鼠血漿中人參皂苷Rg3的含量。各組剩余的20只大鼠分別于0.5、1、2、4 h各處死5只,采集心、肝、脾、肺和腎組織,取組織樣品0.5 g,加入甲醇1 ml,組織勻漿,震蕩10 min,超聲10 min,4000rpm離心10 min,收集上清液,高效液相色譜法檢測各時間段各組大鼠心、肝、脾、肺和腎中人參皂苷Rg3的含量。論文二:雄性Wistar大鼠40只,隨機分為空白對照組,模型組,人參皂苷組,針刺組,人參皂苷聯(lián)合針刺組,每組8只。采用強制冷水游泳結(jié)合慢性束縛法進行模型復制。模型復制成功后,針刺組給予針刺“脾俞”穴治療,人參皂苷組給予大鼠尾靜脈注射人參皂苷Rg3,人參皂苷聯(lián)合針刺組,先給大鼠尾靜脈注射人參皂苷Rg3,再給予針刺“脾俞”穴,1次/d,連續(xù)治療7天。末次給藥及針刺后1h,各組大鼠斷尾取血,制作血涂片,采用光學顯微鏡觀察大鼠血清中T淋巴細胞轉(zhuǎn)化率;腹主動脈取血后剪開腹腔摘取脾臟,制作脾細胞懸液,用流式細胞儀檢測大鼠脾臟中T細胞亞群CD_3~+T細胞、CD_4~+T細胞和CD_8~+T細胞的比例,及CD_4~+T/CD_8~+T的比值。論文三:雄性Wistar大鼠40只隨機分為空白對照組,模型組,人參皂苷組,針刺組,人參皂苷聯(lián)合針刺組,每組8只。各組大鼠模型復制及干預結(jié)束后,用10%水合氯醛以3ml/kg體質(zhì)量腹腔注射麻醉,腹主動脈穿刺采集全血,取上清液凍存于-80℃冰箱中,Elisa法檢測血清中IFN-γ和IL-4含量。同時采集大鼠脾臟組織,采用RT-PCR法檢測脾臟中IFN-γ和IL-4 m RNA表達水平,Western Blot法檢測脾臟中IFN-γ和IL-4蛋白表達水平。結(jié)果:論文一:1模型評價:造模前兩組大鼠體重差異無統(tǒng)計學意義(p0.05),造模后與空白對照組比較,模型組體重顯著減少(p0.01),乳酸含量顯著升高(p0.01),說明模型復制成功。2各組大鼠血漿中人參皂苷Rg3含量:與空白對照組同期比較,模型組60min及120min血漿中人參皂苷Rg3的含量顯著降低,空白針刺組60min及120min血漿中人參皂苷Rg3的含量顯著增加(P0.05);與模型組比較,模型針刺組及空白針刺組60min及120min血漿中人參皂苷Rg3的含量顯著增加(P0.05)。在5 min時血漿中人參皂苷Rg3的含量最高,之后逐漸降低。3各組大鼠肝臟中人參皂苷Rg3分布:與空白對照組同期比較,模型組1 h、2 h及4 h人參皂苷Rg3的含量顯著降低(P0.05),空白針刺組1 h、2 h及4 h人參皂苷Rg3的含量顯著增加(P0.05),模型針刺組無明顯變化;與模型組比較,模型針刺組及空白針刺組1 h、2 h及4 h人參皂苷Rg3的含量均顯著增加(P0.05)。1 h時肝臟中人參皂苷Rg3的含量達到峰值,之后逐漸降低。4各組大鼠心臟中人參皂苷Rg3分布:與空白對照組同期比較,模型組0.5 h及1 h人參皂苷Rg3的含量顯著降低(P0.05),空白針刺組0.5 h及1 h人參皂苷Rg3的含量顯著增加(P0.05);與模型組比較,模型針刺組及空白針刺組0.5 h及1 h人參皂苷Rg3的含量均顯著增加(P0.05)。心臟中0.5 h時人參皂苷Rg3的含量最高,之后逐漸降低,四組大鼠心臟組織中4 h時均檢測不到人參皂苷Rg3。5各組大鼠脾臟中人參皂苷Rg3分布:與空白對照組比較,模型組0.5h、1 h、及2h人參皂苷Rg3的含量顯著降低(P0.05),空白針刺組0.5 h、1 h、及2 h人參皂苷Rg3的含量顯著增加(P0.05);與模型組比較,模型針刺組及空白針刺組0.5h、1 h、及2 h人參皂苷Rg3的含量均顯著增加(P0.05)。脾臟中0.5 h時人參皂苷Rg3的含量最高,之后逐漸降低。6各組大鼠肺臟中人參皂苷Rg3分布:與空白對照組同期比較,模型組各時間點人參皂苷Rg3的含量均降低,空白針刺組各時間點人參皂苷Rg3的含量均升高,但均不具有統(tǒng)計學意義(P0.05);與模型組比較,空白針刺組1 h、2 h人參皂苷Rg3的含量顯著增加(P0.05),模型針刺組各時間點人參皂苷Rg3的含量均有增加,但不具有統(tǒng)計學意義(P0.05),四組大鼠肺臟組織中4 h時均檢測不到人參皂苷Rg3。7各組大鼠腎臟中人參皂苷Rg3分布:與空白對照組同期比較,模型組2 h及4 h人參皂苷Rg3的含量顯著降低(P0.05),空白針刺組2 h及4 h人參皂苷Rg3的含量顯著增加(P0.05);與模型組比較,模型針刺組及空白針刺組2 h及4 h人參皂苷Rg3的含量均顯著增加(P0.05)。腎臟中1 h時人參皂苷Rg3的含量達到峰值,之后逐漸降低。論文二:1各組大鼠淋巴細胞轉(zhuǎn)化率結(jié)果:與空白對照組比較,各組大鼠淋巴細胞轉(zhuǎn)化率顯著降低(P0.01);與模型組比較,人參皂苷組,針刺組及人參皂苷聯(lián)合針刺組淋巴細胞轉(zhuǎn)化率顯著升高(P0.01);與人參皂苷聯(lián)合針刺組比較,人參皂苷組和針刺組淋巴細胞轉(zhuǎn)化率顯著降低(P0.05);而人參皂苷組與針刺組之間比較無統(tǒng)計學意義(P0.05)。未轉(zhuǎn)化的淋巴細胞呈正圓形,細胞核呈紫色,正圓形;胞漿呈淡藍色極少,呈新月形位于細胞邊緣。發(fā)生轉(zhuǎn)化的淋巴母細胞個體增大,細胞核變大,偶見核仁出現(xiàn),形態(tài)不規(guī)則,胞漿明顯增多,胞漿內(nèi)偶見空泡出現(xiàn)。2流式細胞結(jié)果:CD_3~+T細胞比例結(jié)果:與空白對照組比較,模型組CD_3~+T細胞比例顯著降低(P0.01),人參皂苷組CD_3~+T細胞比例顯著降低(P0.05);與模型組比較,針刺組CD_3~+T細胞比例顯著升高(P0.01),人參皂苷聯(lián)合針刺組CD_3~+T細胞比例顯著升高(P0.05);而人參皂苷組與針刺組之間比較無統(tǒng)計學意義(P0.05)。CD_4~+T細胞比例結(jié)果:與空白對照組比較,模型組CD_4~+T細胞比例升高,差異有統(tǒng)計學意義(P0.05);與模型組比較,人參皂苷聯(lián)合針刺組CD_4~+T細胞比例降低,差異有統(tǒng)計學意義(P0.05)。CD_8~+T細胞比例結(jié)果:與空白對照組比較,各組大鼠CD_8~+T細胞比例顯著降低(P0.01);與模型組比較,針刺組、人參皂苷組、人參皂苷聯(lián)合針刺組CD_8~+T細胞比例均顯著升高(P0.01);與人參皂苷聯(lián)合針刺組比較,人參皂苷組CD_8~+T細胞比例顯著降低(P0.01)。CD_4~+T/CD_8~+T結(jié)果:與空白對照組比較,模型組、皂苷組CD_4~+T/CD_8~+T比值均顯著升高(P0.01);與模型組比較,針刺組、人參皂苷組及人參皂苷聯(lián)合針刺組CD_4~+T/CD_8~+T比值顯著降低(P0.01)。與人參皂苷聯(lián)合針刺組比較,人參皂苷組CD_4~+T/CD_8~+T比值顯著升高,(P0.05)。論文三:1各組大鼠血清中IFN-γ和IL-4含量結(jié)果:與空白對照組比較,各組大鼠血清中IFN-γ含量顯著降低(P0.01);與模型組比較,針刺組、皂苷組、人參皂苷聯(lián)合針刺組IFN-γ含量顯著升高(P0.01);與人參皂苷聯(lián)合針刺組比較,針刺組、人參皂苷組IFN-γ含量顯著降低(P0.01);而人參皂苷組與針刺組之間比較無統(tǒng)計學意義(P0.05)。與空白對照組比較,各組大鼠血清中IL-4顯著升高(P0.01);與模型組比較,針刺組、皂苷組、人參皂苷聯(lián)合針刺組IL-4含量顯著降低(P0.01);與人參皂苷聯(lián)合針刺組比較,針刺組、人參皂苷組IL-4含量顯著升高(P0.01);而人參皂苷組與針刺組之間比較無統(tǒng)計學意義(P0.05)。2各組大鼠脾臟中IFN-γ和IL-4的蛋白表達水平結(jié)果:與空白對照組比較,各組大鼠IFN-γ蛋白表達水平顯著降低(P0.01);與模型組比較,針刺組、皂苷組、人參皂苷聯(lián)合針刺組IFN-γ蛋白表達水平顯著升高(P0.01);與人參皂苷聯(lián)合針刺組比較,針刺組和人參皂苷組IFN-γ蛋白表達水平顯著降低(P0.05);而人參皂苷組與針刺組之間比較無統(tǒng)計學意義(P0.05)。與空白對照組比較,各組大鼠IL-4蛋白表達水平顯著升高(P0.01);與模型組比較,針刺組、皂苷組、人參皂苷聯(lián)合針刺組IL-4蛋白表達顯著水平降低(P0.01);與人參皂苷聯(lián)合針刺組比較,針刺組和人參皂苷組IL-4蛋白表達水平顯著降低(P0.05);而人參皂苷組與針刺組之間比較無統(tǒng)計學意義(P0.05)。3各組大鼠脾臟中IFN-γ和IL-4 m RNA表達水平結(jié)果:與空白對照組比較,各組大鼠IFN-γm RNA表達水平顯著降低(P0.01);與模型組比較,針刺組、皂苷組、人參皂苷聯(lián)合針刺組IFN-γm RNA表達水平顯著升高(P0.01);與人參皂苷聯(lián)合針刺組比較,針刺組和人參皂苷組IFN-γm RNA表達水平顯著降低(P0.05);而人參皂苷組與針刺組之間比較無統(tǒng)計學意義(P0.05)。與空白對照組比較,各組大鼠IL-4 m RNA表達水平顯著升高(P0.01);與模型組比較,針刺組、皂苷組、人參皂苷聯(lián)合針刺組IL-4 m RNA表達水平顯著降低(P0.01);與人參皂苷聯(lián)合針刺組比較,針刺組和人參皂苷組IL-4 m RNA表達水平顯著升高(P0.05);而人參皂苷組與針刺組之間比較無統(tǒng)計學意義(P0.05)。結(jié)論:1.針刺“脾俞”穴可以增加人參皂苷Rg3在血內(nèi)的含量和在五臟中的分布,延長藥物在體內(nèi)的停留時間,這可能是針藥并用協(xié)同增效的物質(zhì)基礎(chǔ)。2.針刺“脾俞”穴和靜脈注射人參皂苷Rg3是通過糾正慢性疲勞模型大鼠Th1/Th2失衡狀態(tài),調(diào)節(jié)T淋巴細胞亞群,增強機體細胞免疫功能實現(xiàn)其抗疲勞作用。3.針刺“脾俞”穴和靜脈注射人參皂苷Rg3均具有抗疲勞作用,并且二者聯(lián)用具有協(xié)同增效作用,為針藥并用協(xié)同增效抗疲勞提供實驗理論基礎(chǔ)。
[Abstract]:Objective: This study by high performance liquid chromatography method was used to observe the effect of acupuncture points on the distribution and metabolism of spleen Shu "of ginsenoside Rg3 in chronic fatigue rat model and observe the viscera, T lymphocyte transformation rate in rat serum by optical microscopy, rat spleen cells were detected by flow cytometry in T cell subsets of CD_3~+T cells, CD_4~+T cells and CD_8~+T cell ratio, CD_4~+/CD_8~+ ratio, IFN- content changes of gamma and IL-4 in serum were measured by ELISA, RT-PCR in spleen was detected in IFN- gamma and IL-4 m expression level of RNA, using Western Blot method to detect spleen IFN- gamma and IL-4 protein expression, to investigate the effects of Acupuncture on Pishu points combined with ginsenoside Rg3 the combination of acupuncture and medicine," the mechanism of anti fatigue yiqijianpi. Materials and methods: Part One: 116 male Wistar rats were randomly divided into 4 groups: control group (33 rats), model group (33 rats), the blank plus acupuncture group (25 Only), model plus acupuncture group (25 rats). The forced cold water swimming with chronic restraint method for model replication. The modeling time 21d.21d, model group and blank control group rats were randomly selected 8 rats by detecting the rats in body weight and serum lactate content changes to determine the success of modeling. The blank group and model plus acupuncture plus acupuncture group in the model was established successfully after treated by acupuncture "Pishu" acupuncture treatment, the needle after the needle handle electric acupuncture apparatus, voltage 4.5 V, frequency 4/20 Hz dilatational wave frequency, intensity of local skin and muscle to rats of tremor the needle, 20 min, 1 /d, seventh days after acupuncture treatment, rats were intravenously injected with ginsenoside Rg3. rats were 5 rats after administration of 5,30,60120 min by rat orbital blood 2ml in heparinized centrifuge tubes, 3000 rpm centrifugation 10 min, plasma separation, HPLC color The spectral content of plasma was detected in different time of rats in ginsenoside Rg3. 20 rats in each group were 0.5,1,2,4 h in the remaining 5 rats of each group were sacrificed to collect heart, liver, spleen, lung, and renal tissue, the tissue samples of 0.5 g, add 1 ml of methanol, homogenate, shock 10 min, ultrasound 10 min 4000rpm centrifugation for 10 min, the supernatants were detected in different time of rats heart, HPLC liver, spleen, lung and kidney in the content of ginsenoside Rg3. The two: 40 male Wistar rats were randomly divided into control group, model group, ginsenoside group, needle ginsenosides combined with acupuncture group, acupuncture group, 8 rats in each group. The forced cold water swimming with chronic restraint method for model replication. When the model was established successfully, the acupuncture group was given acupuncture "Pishu" acupuncture therapy, ginsenoside group rats were given intravenous injection of ginsenoside Rg3, ginsenoside combined with acupuncture group, give rats teleutostatic Intravenous injection of ginsenoside Rg3, then treated by acupuncture Pishu points, 1 times /d, for 7 consecutive days. The last drug and acupuncture on 1H rats, tail blood, making blood smears were observed by optical microscopy in rat serum T lymphocyte transformation rate; blood from the abdominal aorta after cut the removal of abdominal spleen, make spleen cell suspension were detected by using flow cytometry in rat spleen T cell subsets of CD_3~+T cells, CD_4~+T cells and CD_8~+T cell ratio and CD_4~+T/CD_8~+T ratio. The three: 40 male Wistar rats were randomly divided into control group, model group, ginsenoside group, acupuncture ginsenoside group, acupuncture group, 8 rats in each group. The rats model of replication and after intervention with 10% chloral hydrate with 3ml/kg body weight intraperitoneal injection of anesthesia, abdominal aortic blood sampling puncture, the supernatant was frozen in -80 C refrigerator, Elisa were detected by IFN- and I The content of L-4. At the same time from spleen tissues of rats was detected by RT-PCR in the spleen of IFN- gamma and IL-4 m RNA expression level of Western in spleen was detected by Blot IFN- gamma and IL-4 protein expression. Results: Part One: 1 model evaluation: before modeling the two groups of rats had no significant difference (P0.05) after modeling, compared with the control group, the weight of the model group decreased significantly (P0.01), lactic acid content increased significantly (P0.01), the model successfully copied the plasma.2 of rats in ginsenoside Rg3 content: compared with the blank control group, model group were 60min and 120min in plasma of ginsenoside Rg3 decreased significantly. The content of 60min in the acupuncture group and blank plasma 120min in ginsenoside Rg3 increased significantly (P0.05); compared with the model group, the content of the model group and the blank acupuncture acupuncture group of 60min and 120min in plasma of ginsenoside Rg3 increased significantly (P0.05). At 5 min in human plasma Ginsenoside Rg3 was the highest, then gradually reduce the distribution of ginsenoside Rg3.3 in liver of rats: compared with the blank control group, model group, 1 h, 2 h and 4 h the content of ginsenoside Rg3 decreased significantly (P0.05), blank acupuncture group 1 h, 2 h and 4 h the content of Ginsenoside Rg3 increased significantly (P0.05) model, the acupuncture group had no obvious change; compared with the model group, model group and blank acupuncture acupuncture group of 1 h, 2 h and 4 h the content of ginsenoside Rg3 significantly increased the content of.1 (P0.05) h in the liver of ginsenoside Rg3 reached the peak, then gradually decreased distribution of Rg3 ginsenoside.4 heart of rats in each group: compared with the blank control group, model group was 0.5 h and 1 h of ginsenoside Rg3 decreased significantly (P0.05), the content of blank acupuncture group of 0.5 h and 1 h of ginsenoside Rg3 increased significantly (P0.05); compared with the model group, model group and blank acupuncture acupuncture group of 0.5 h and 1 h The content of ginsenoside Rg3 increased significantly (P0.05). In the heart of 0.5 h of ginsenoside Rg3 content is the highest, then gradually decreased, the heart tissue in rats of the four groups in 4 h were not detected in the distribution of ginsenoside Rg3 spleen of ginsenoside Rg3.5 in rats: compared with the blank control group, 0.5h model group 1 h, 2h and content of ginsenoside Rg3 decreased significantly (P0.05), blank acupuncture group 0.5 h, 1 h, 2 h and the content of ginsenoside Rg3 increased significantly (P0.05); compared with the model group, acupuncture group, acupuncture group and blank model 0.5h, 1 h, 2 h and the content of ginsenoside Rg3 the increased significantly (P0.05). In the spleen of 0.5 h ginsenoside Rg3 content is the highest, then gradually reduce the distribution of Rg3 ginsenoside.6 in the lung of rats in each group: compared with the blank control group, model group in each time point of ginsenoside Rg3 decreased, the content of blank acupuncture group at each time point of ginsenoside Rg3 the Both increased, but were not statistically significant (P0.05); compared with the model group, blank acupuncture group 1 h, 2 h the content of ginsenoside Rg3 increased significantly (P0.05), were content model acupuncture group at different time points of ginsenoside Rg3 increased, but not statistically significant (P0.05), four groups of rats the lung tissue of 4 h were not detected in the distribution of ginsenoside Rg3 in kidney of ginsenoside Rg3.7 in rats of each group: compared with the blank control group, model group was 2 h and 4 h of ginsenoside Rg3 decreased significantly (P0.05), the content of blank acupuncture group of 2 h and 4 h of ginsenoside Rg3 increased significantly (P0.05); compared with the model group, the content of the model group and the blank acupuncture acupuncture group of 2 h and 4 h of ginsenoside Rg3 were significantly increased (P0.05) in the kidney of 1 h. The content of ginsenoside Rg3 reached the peak, then decreased gradually. The two rats in each group: 1 lymphoid cell conversion rate results: compared with the blank the control group Comparison of lymphocytes of rats significantly decreased the conversion rate (P0.01); compared with the model group, ginsenoside group, acupuncture group and acupuncture group of ginsenosides combined with the lymphocyte transformation rate was significantly increased (P0.01); compared with ginsenosides combined with acupuncture group, ginsenoside group and acupuncture group significantly decreased the rate of lymphocyte transformation (P0.05); between ginsenoside group and acupuncture group was not statistically significant (P0.05). Positive lymphocytes unconverted round nuclei were round, purple, pale blue cytoplasm; rarely, crescent shaped at the edge of the cells. The transformed lymphoblastoid cell nucleus became large, individual increases, occasionally nucleolus, morphology the rules, the cytoplasm increased significantly, cytoplasmic vacuolization occasionally.2 flow cytometry. Results: the percentage of CD_3~+T cells results: compared with the blank control group, model group of CD_3~+T cells was significantly lower than patients (P0.01), ginsenoside CD_3 group The proportion of ~+T cells decreased significantly (P0.05); compared with the model group, the acupuncture group was significantly increased CD_3~+T cell ratio (P0.01), ginsenosides combined with acupuncture group the percentage of CD_3~+T cells increased significantly (P0.05); and between ginsenoside group and acupuncture group had no significant difference between the percentage of.CD_4~+T cells (P0.05) results: compared with the control group group, the proportion of CD_4~+T cell model increased, the difference was statistically significant (P0.05); compared with the model group, the acupuncture group of ginsenosides combined with CD_4~+T were decreased, the difference was statistically significant (P0.05.CD_8) ~+T cell ratio results: compared with the control group, the proportion of CD_8~+T cells of each group rats were significantly decreased (P0.01); comparison with the model group, acupuncture group, ginsenoside group, acupuncture group the proportion of CD_8~+T cells combined with ginsenoside were significantly increased (P0.01); compared with ginsenosides combined with acupuncture group, ginsenoside group the proportion of CD_8~+T cells significantly Reduce (P0.01).CD_4~+T/CD_8~+T results: compared to the control group, model group and blank group, CD_4~+T/CD_8~+T saponins were significantly higher (P0.01); compared with the model group, acupuncture group, ginsenoside group and ginsenoside CD_4~+T/CD_8~+T combined with acupuncture group was significantly lower (P0.01). Compared with the ginsenosides combined with acupuncture group, ginsenoside group the ratio of CD_4~+T/CD_8~+T increased significantly (P0.05). The three: 1 in serum IFN- gamma and IL-4 content results: compared with the control group, IFN- content in serum of rats were significantly decreased (P0.01); compared with the model group, acupuncture group, saponin group, ginsenoside IFN- combined with acupuncture group gamma content increased significantly (P0.01); compared with ginsenosides combined with acupuncture group, acupuncture group, ginsenoside IFN- group gamma content decreased significantly (P0.01); and between ginsenoside group and acupuncture group had no statistical significance (P0.05) and blank control. Group, IL-4 in serum of rats were significantly increased (P0.01); compared with the model group, acupuncture group, saponin group, ginsenoside IL-4 combined with acupuncture group was significantly lower (P0.01); compared with ginsenosides combined with acupuncture group, acupuncture group, ginsenoside IL-4 group was significantly increased (P0.01); and between ginseng the saponin group and acupuncture group had no statistical significance (P0.05) expression of IFN- gamma and IL-4.2 in spleen of rats in protein level. Results: compared with the control group, the expression level of IFN- protein gamma groups rats were significantly decreased (P0.01); compared with the model group, acupuncture group, saponin group, the expression level of ginsenosides combined with the acupuncture group IFN- gamma protein increased significantly (P0.01); compared with ginsenosides combined with acupuncture group, the expression level of acupuncture group and ginsenoside group IFN- gamma protein decreased significantly (P0.05); and ginsenoside group and acupuncture group were no statistical significance (P0.05) and empty. White compared to the control group, the expression level of IL-4 protein in rats were significantly increased (P0.01); compared with the model group, acupuncture group, acupuncture group saponin group, expression of IL-4 protein significantly decreased levels of ginsenoside (P0.01); compared with ginsenosides combined with acupuncture group, acupuncture group and ginsenoside group the expression level of IL-4 protein significantly reduce (P0.05); and between ginsenoside group and acupuncture group was not statistically significant (P0.05) level and IL-4 m results IFN- gamma RNA expression in spleen of.3 rats compared with control group, the expression of IFN- gamma m rats RNA levels decreased significantly (P0.01); compared with the model group, acupuncture group, saponin group, ginsenoside IFN- combined with acupuncture group gamma m RNA expression level was significantly increased (P0.01); compared with ginsenosides combined with acupuncture group, the expression level of acupuncture group and ginsenoside IFN- gamma m RNA group decreased significantly (P0.05); and ginsenoside group and acupuncture group. There was no significant difference (P0.05). Compared with the control group, the expression level of IL-4 m in RNA rats increased significantly (P0.01); compared with the model group, acupuncture group, saponin group, the expression level of ginsenosides combined with acupuncture group IL-4 m RNA decreased significantly (P0.01); compared with ginsenoside combined with acupuncture group. The expression level of acupuncture group and ginsenoside IL-4 group M RNA were significantly increased (P0.05); and between ginsenoside group and acupuncture groups were not statistically significant (P0.05). Conclusion: the contents of 1. acupuncture Pishu points can increase the ginsenoside Rg3 in the blood and in the internal organs in the distribution, prolong the retention time of medicine in in vivo, which may

【學位授予單位】：遼寧中醫(yī)藥大學
【學位級別】：博士
【學位授予年份】：2016
【分類號】：R245

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