本文關鍵詞：電針聯(lián)合跑臺訓練對大鼠骨骼肌鈍挫傷的修復作用　出處：《針刺研究》2017年05期 　論文類型：期刊論文

  更多相關文章： 骨骼肌鈍挫傷 電針聯(lián)合跑臺 哺乳動物雷帕霉素靶蛋白 肌細胞生成素 快肌型骨骼肌肌球蛋白重鏈

【摘要】：目的:觀察電針聯(lián)合跑臺訓練對大鼠骨骼肌鈍挫傷的修復作用,探討電針聯(lián)合跑臺訓練改善骨骼肌損傷的可能機制。方法:SD大鼠隨機分為正常組、模型組、電針組、跑臺組和聯(lián)合組,每組12只。采用自制打擊裝置建立骨骼肌鈍挫傷模型。電針組電針"足三里"、阿是穴,跑臺組進行跑臺訓練,聯(lián)合組同時進行電針和跑臺訓練,1次/d,連續(xù)至損傷后3、14d。HE染色觀察損傷后14d腓腸肌新生肌細胞橫截面積和直徑,Western blot法檢測損傷后3d腓腸肌哺乳動物雷帕霉素靶蛋白(mTOR)、肌細胞生成素(myoG)和14d腓腸肌mTOR、快肌型骨骼肌肌球蛋白重鏈(Fast MyHC)的表達量。結果:模型組新生肌細胞橫截面積和直徑明顯小于正常組(P0.01),各治療組均顯著大于模型組(P0.05),聯(lián)合組顯著大于電針組和跑臺組(P0.05)。與正常組比較,損傷后3d模型組腓腸肌mTOR和myoG蛋白表達均顯著升高(P0.01);與模型組比較,各治療組腓腸肌mTOR和myoG蛋白表達均顯著升高(P0.01);與電針組比較,跑臺組腓腸肌mTOR和myoG蛋白表達均顯著降低(P0.01);與電針組和跑臺組比較,聯(lián)合組腓腸肌mTOR和myoG蛋白表達顯著升高(P0.05,P0.01)。與正常組比較,損傷后14d模型組腓腸肌mTOR蛋白表達升高,但差異無統(tǒng)計學意義(P0.05),Fast MyHC蛋白表達顯著降低(P0.01);與模型組比較,各治療組腓腸肌mTOR及Fast MyHC蛋白表達均顯著升高(P0.05,P0.01);與電針組比較,跑臺組腓腸肌mTOR及Fast MyHC蛋白表達均顯著降低(P0.05,P0.01);與電針組和跑臺組比較,聯(lián)合組腓腸肌mTOR及Fast MyHC蛋白表達均顯著升高(P0.01)。結論:電針聯(lián)合跑臺訓練能促進成肌細胞分化成熟,減輕骨骼肌損傷,該作用與增加mTOR表達量,正向調控myoG、Fast MyHC的表達有關。
[Abstract]:Objective: To observe the effect of electroacupuncture combined with rehabilitation training on the run and skeletal muscle contusion of rats, and explore the possible mechanism of electroacupuncture combined with treadmill training improves skeletal muscle injury. Methods: SD rats were randomly divided into normal group, model group, electroacupuncture group, running group and combination group, 12 rats in each group. Using the self-made striking device skeletal muscle contusion model. The electroacupuncture group and Electroacupuncture of "Zusanli", Ashi, treadmill treadmill training group, combination group and electroacupuncture and treadmill training, 1 /d, continuous to staining 14d after injury of gastrocnemius muscle cells of newborn cross-sectional area and the diameter of 3,14d.HE after injury, Western blot detection of 3D after injury of gastrocnemius muscle of the mammalian target of rapamycin (mTOR), myogenin (myoG) and 14d gastrocnemius mTOR, fast twitch skeletal muscle type myosin heavy chain (Fast MyHC) expression. Results: the model group of new muscle cell cross-sectional area and diameter significantly Less than the normal group (P0.01), the treatment group were significantly higher than the model group (P0.05), combination group was significantly higher than that in the electroacupuncture group and treadmill group (P0.05). Compared with the normal group, the expression of 3D after injury in model group gastrocnemius mTOR and myoG protein were significantly increased (P0.01); compared with the model group, the expression of the treatment group gastrocnemius mTOR and myoG protein were significantly increased (P0.01); compared with the electroacupuncture group, running group gastrocnemius mTOR and myoG protein expression were significantly decreased (P0.01); and the electroacupuncture group and treadmill group compared with gastrocnemius mTOR and myoG protein expression was significantly increased (P0.05, P0.01). With the normal group, model group, 14d expression of gastrocnemius muscle mTOR protein increased after injury, but the difference was not statistically significant (P0.05), the expression of Fast MyHC protein was significantly decreased (P0.01); compared with the model group, the expression of each treatment group mTOR and Fast MyHC protein in the gastrocnemius muscle were significantly increased (P0.05, P0.01); compared with the electroacupuncture group run, Taiwan Group mTOR and Fast gastrocnemius muscle MyHC protein expression were significantly decreased (P0.05, P0.01); and the electroacupuncture group and treadmill group compared with group mTOR and Fast gastrocnemius muscle MyHC protein expression increased significantly (P0.01). Conclusion: electroacupuncture combined with treadmill training can promote myoblast differentiation and maturation, reduce the injury of skeletal muscle this effect, with the increase of mTOR expression, the positive regulation of myoG, the expression of Fast MyHC.

【作者單位】： 重慶醫(yī)科大學中醫(yī)藥學院;
【基金】：國家自然科學基金項目(No.81403466、81273870) 重慶市渝中區(qū)項目(No.20140117) 重慶市衛(wèi)計委項目(No.ZY 20150250) 重慶市教委項目(No.KJ 1702033) 重慶市體育局項目(No.B 201408)
【分類號】：R245
【正文快照】： 急性骨骼肌鈍挫傷是骨骼肌運動性損傷中最常見的類型之一[1],以肌纖維的斷裂和溶解為主要病變,其病程長且預后差,導致骨骼肌的結構和功能遭到損害,嚴重影響患者的生活質量,因此骨骼肌損傷后的康復治療是該領域研究的重點。研究表明建立動物急性骨骼肌鈍挫傷模型,對研究骨骼肌

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