發(fā)布時(shí)間：2017-12-27 17:03

  本文關(guān)鍵詞：JMJD2在腎虛型絕經(jīng)后骨質(zhì)疏松癥患者骨組織中表達(dá)分析　出處：《廣州中醫(yī)藥大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 絕經(jīng)后骨質(zhì)疏松癥 組蛋白去甲基化酶 雌激素受體α 雌激素受體β 骨密度

【摘要】：目的：觀察腎虛型絕經(jīng)后骨質(zhì)疏松癥患者骨組織中組蛋白去甲基化酶JMJD2A、 JMJD2B和雌激素受體ERα.ERβ的基因及蛋白表達(dá)水平變化,并分析組蛋白去甲基化酶JMJD2A、JMJD2B與骨密度的相關(guān)性以及JMJD2A、JMJD2B與ERα、ERβ的相關(guān)性,以期發(fā)現(xiàn)組蛋白去甲基化酶JMJD2與腎虛型絕經(jīng)后骨質(zhì)疏松癥的聯(lián)系,為治療絕經(jīng)后骨質(zhì)疏松癥尋找新的靶點(diǎn)。方法：選擇于2014年10月至2015年6月廣州中醫(yī)藥大學(xué)第一附屬醫(yī)院骨科收治的因髖關(guān)節(jié)骨性關(guān)節(jié)炎行全髖關(guān)節(jié)置換術(shù)的絕經(jīng)后女性患者30例,年齡在50歲至70歲,術(shù)前使用雙能X線骨密度儀測(cè)量患者術(shù)側(cè)股骨頸骨密度,分為對(duì)照組15例(即骨量正常組,股骨頸BMD T值-1.0)和實(shí)驗(yàn)組15例(即絕經(jīng)后骨質(zhì)疏松組,股骨頸BMD T值≤-2.5),術(shù)中取術(shù)側(cè)股骨頸處松質(zhì)骨備用。應(yīng)用熒光實(shí)時(shí)定量PCR和Western blot法分別檢測(cè)松質(zhì)骨骨組織中JMJD2A、JMJD2B、ERα和ER β基因及蛋白的表達(dá)水平。實(shí)驗(yàn)數(shù)據(jù)使用SPSS20.0軟件處理,P0.05認(rèn)定為有統(tǒng)計(jì)學(xué)意義。采用Pearson相關(guān)性分析法分析JMJD2A、JMJD2B基因及蛋白表達(dá)與骨密度的相關(guān)性,分析JMJD2A、JMJD2B 與 ER α、ERβ基因及蛋白表達(dá)之間的相關(guān)性。結(jié)果：1、與骨量正常組相比,絕經(jīng)后骨質(zhì)疏松組骨組織中JMJD2A、JMJD2B、 ERα及ER β基因及蛋白表達(dá)水平均明顯下降,組間差異具有統(tǒng)計(jì)學(xué)意義(P值均0.01)。與骨量正常組相比,絕經(jīng)后骨質(zhì)疏松組骨組織中OPG mRNA表達(dá)水平下降,RANKL及RANKL/OPG mRNA表達(dá)水平均升高,兩組間差異具有統(tǒng)計(jì)學(xué)意義(P值均0.01)。2、相關(guān)性分析顯示絕經(jīng)后女性股骨頸骨密度與JMJD2A、JMJD2B的基因和蛋白表達(dá)水平成顯著正相關(guān)；ERα、ERβ分別與JMJD2A、JMJD2B的基因及蛋白表達(dá)水平成顯著正相關(guān)。結(jié)論：1、絕經(jīng)后骨質(zhì)疏松組骨組織中組蛋白去甲基化酶JMJD2A、JMJD2B表達(dá)水平明顯低于骨量正常組,骨密度與JMJD2A、JMJD2B表達(dá)水平成顯著正相關(guān),提示組蛋白去甲基化酶JMJD2A、JMJD2B可能參與骨代謝過程,能夠降低絕經(jīng)后骨質(zhì)疏松癥的發(fā)生。2、兩組患者骨組織中ER α、ER β表達(dá)水平與組蛋白去甲基化酶JMJD2A、JMJD2B存在顯著正相關(guān),提示JMJD2A和JMJD2B可能通過調(diào)控雌激素-ER信號(hào)通路調(diào)節(jié)骨代謝,它們之間的具體交通機(jī)制有待闡明。3、組蛋白去甲基化酶JMJD2A、JMJD2B可能作為腎虛型絕經(jīng)后骨質(zhì)疏松癥的一種括抗酶。
[Abstract]:Objective: the expression levels of histone bone in osteoporosis to gene and protein methylation enzymes JMJD2A, JMJD2B and estrogen receptor alpha.ER beta ER observation of kidney deficiency after menopause, and analysis of histone demethylase JMJD2A, JMJD2B and bone mineral density and the correlation between JMJD2A, JMJD2B and ER alpha, ER beta in order to find the correlation, histone methyltransferase JMJD2 in postmenopausal osteoporosis with kidney deficiency after contact, as a new target for the treatment of postmenopausal osteoporosis. Methods: in the Department of orthopedics of the First Affiliated Hospital of Guangzhou University of Chinese Medicine from October 2014 to June 2015 30 cases of postmenopausal female patients with hip osteoarthritis undergoing total hip replacement, aged 50 to 70 years of age, preoperative use of dual energy X-ray absorptiometry measurement in patients with femoral neck BMD, divided into control group of 15 cases (i.e. normal bone mass group, femoral neck BMD T value -1.0) and 15 cases in the experimental group (i.e. postmenopausal osteoporosis group, femoral neck BMD T value is less than or equal to -2.5), extraction operation side at the femoral neck cancellous bone reserve. The expression of JMJD2A, JMJD2B, ER -, and ER beta gene and protein in cancellous bone tissue was detected by real time fluorescence quantitative PCR and Western blot. The experimental data were treated with SPSS20.0 software, and P0.05 was found to be statistically significant. Pearson correlation analysis was used to analyze the correlation between JMJD2A and JMJD2B gene and protein expression and bone mineral density, and to analyze the correlation between JMJD2A, JMJD2B and ER alpha, ER beta gene and protein expression. Results: 1. Compared with normal bone mass group, the expression levels of JMJD2A, JMJD2B, ER and ER ER in bone tissue of postmenopausal osteoporosis group were significantly decreased, and the difference between groups was statistically significant (P = 0.01). Compared with the normal bone mass group, the expression level of OPG mRNA in the bone tissue of postmenopausal osteoporosis group decreased, and the expression level of RANKL and RANKL/OPG mRNA increased. The difference between the two groups was statistically significant (P = 0.01). 2, correlation analysis showed that BMD of femur neck was positively correlated with JMJD2A and JMJD2B gene and protein expression level in postmenopausal women, and ER and ER were significantly positively correlated with JMJD2A and JMJD2B gene and protein expression level. Conclusion: the osteoporosis bone tissue, 1 group of histone demethylase JMJD2A after menopause, the expression level of JMJD2B was significantly lower than that of normal bone mass group, the expression of bone mineral density and JMJD2A, JMJD2B level was positively correlated, suggesting that histone demethylase JMJD2A and JMJD2B may be involved in bone metabolism process, can reduce the incidence of osteoporosis postmenopausal. 2, two groups of patients in bone tissue ER alpha, ER beta expression level and histone demethylation JMJD2A and JMJD2B have a significant positive correlation, suggesting that JMJD2A and JMJD2B may regulate the bone metabolism through estrogen -ER signaling pathway, and the specific traffic mechanism between them needs to be elucidated. 3. Histone demethylation enzyme JMJD2A and JMJD2B may be an anti enzyme of kidney deficiency postmenopausal osteoporosis.
【學(xué)位授予單位】：廣州中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R259

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張智海;劉忠厚;李娜;張萌萌;黃琪仁;馬遠(yuǎn)征;王亮;劉勇;劉新宇;朱鈞;藍(lán)旭;李士春;楊鴻兵;喻恒峰;湯光宇;張偉;姚偉武;李紹林;彭俊紅;周晟;周勁松;;中國人骨質(zhì)疏松癥診斷標(biāo)準(zhǔn)專家共識(shí)(第三稿·2014版)[J];中國骨質(zhì)疏松雜志;2014年09期

2 楊明園;李超;李明;;細(xì)胞因子與絕經(jīng)后骨質(zhì)疏松癥關(guān)系的研究進(jìn)展[J];中國骨質(zhì)疏松雜志;2014年06期

3 胡志俊;王世偉;劉文波;陳向東;何桂群;;骨質(zhì)疏松的中醫(yī)辨證分型研究[J];中國中醫(yī)骨傷科雜志;2012年01期

4 徐莉;謝冠群;溫成平;范永升;;中藥及中藥有效成分對(duì)表觀遺傳作用的研究進(jìn)展[J];中華中醫(yī)藥雜志;2011年07期

5 郭華平;郁嫣嫣;陳文華;余波;祁奇;;絕經(jīng)后骨質(zhì)疏松癥發(fā)病相關(guān)危險(xiǎn)因素分析及預(yù)防措施探討[J];中國康復(fù)醫(yī)學(xué)雜志;2011年05期

6 ;原發(fā)性骨質(zhì)疏松癥診治指南(2011年)[J];中華骨質(zhì)疏松和骨礦鹽疾病雜志;2011年01期

7 康然;謝林;賈晉輝;洪友松;周中;劉光旺;王庚啟;;女性原發(fā)性骨質(zhì)疏松癥患者年齡、絕經(jīng)年限與不同部位骨密度的關(guān)系[J];實(shí)用中西醫(yī)結(jié)合臨床;2009年05期

8 鄧士琳;李愛民;;生活方式與絕經(jīng)后骨質(zhì)疏松癥發(fā)病風(fēng)險(xiǎn)的關(guān)系[J];華中科技大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2009年04期

9 賀良;鐘偉;李寧;;骨質(zhì)疏松性骨折患者住院治療費(fèi)用(2000~2006年)統(tǒng)計(jì)[J];實(shí)用骨科雜志;2009年05期

10 楊洪彬;徐祖健;汪新象;尹思源;唐光才;劉都;陳巍;;絕經(jīng)后女性骨質(zhì)疏松癥中醫(yī)證型及證候分布規(guī)律的研究[J];四川中醫(yī);2009年04期

，

本文編號(hào)：1342415