本文關(guān)鍵詞：杏鮑菇多糖WPP2的結(jié)構(gòu)表征及抗腫瘤活性，由筆耕文化傳播整理發(fā)布。

杏鮑菇多糖WPP2的結(jié)構(gòu)表征及抗腫瘤活性

Structure Characterization and Antitumor Activity of Polysaccharide WPP2 from Pleurotus Eryngii

[1] [2] [3] [4] [5] [6] [7]

ZHANG Hua-Peng1 , ZHANG Jing1. , NAN Zheng2, SUN Run-Guang2 , ZHANG Pengl LIU A-Juan1 , LIANG Tao1 （ 1. College of Food Engineering and Nutrition Science, 2. College o

[1]陜西師范大學(xué)食品工程與營養(yǎng)科學(xué)學(xué)院; [2]物理學(xué)與信息技術(shù)學(xué)院,西安710062

文章摘要：采用熱水浸提法從杏鮑菇中提取粗多糖，經(jīng)純化得到一種新的多糖WPP2．通過紫外光譜、色譜、質(zhì)譜、核磁共振、紅外光譜、環(huán)境掃描電子顯微鏡（ESEM）和原子力顯微鏡（AFM）等技術(shù)相結(jié)合的方法，對WPP＇2結(jié)構(gòu)進行了表征，并對其體外抗氧化和抗腫瘤活性進行了初步探討．結(jié)果表明，WPP2的平均分子量為4．499x10^-5，主要由Glc組成，每個重復(fù)單元存在2條支鏈，-1，2，3）β-D-Manp和-1，2，6）β-D-Manp位于支鏈位點，支鏈由-1，6）β-D-Glcp和-1，6）β-D-Galp構(gòu)成，非還原末端基為-1）β-D-Glcp，主鏈主要由-1，3）β-D-Glcp，-1，，6）β-D-Galp和-1，3）β-D-Manp糖殘基組成．剛果紅實驗結(jié)果表明其具有三螺旋結(jié)構(gòu)；用ESEM和AFM首次在納米尺度觀察到WPP2具有多分枝結(jié)構(gòu)；活性實驗結(jié)果表明WPP2具有-定的抗氧化和抗腫瘤活性．

Abstr：Polysaccharides are an important class of active components from Pleurotus eryngii with potential biological activities. Scince structure and functions are intimately related, an in-depth study of Pleurotus eryn gii polysaccharides would be of interest. In this work, crude polysaccharides were extracted from Pleurotus er yngii by hot water and were further fractionated to give a new polysaccharide fraction termed WPP2. WPP2 was subjected to structural identification by means of UV, HPLC, GC, IR, GC-MS, NMR, ESEM and AFM. In addition, the antioxidant and antitumor activities of WPP2 were evaluated in vitro. The results revealed that WPP2 is rich in Glc with an average molecular weight of about 4.499 x 105. The main chain of WPP2 was made up of ---1,3 ） -β-D-Glcp, ---1,6） -β-D-Galp, and --＋l, 3 ） -β-D-Manp with two side chains. ---1,6 ） -/3-D- Glcp, ---1,6）-β-D-Galp and terminal residue --＋1 ）-β-D-Glcp were linked to form side chains bearing --1,2, 3 ）-β-D-Manp and ---1,2,6）-β-D-Manp residues as branched points. Congo red reaction indicated that WPP2 has the triple-helical structure in aqueous solution. The multi-branched structure of WPP2 was detected at the nanometer scale by ESEM and AFM; the bioactivity analysis in vitro indicated that WPP2 possessed certain an- tioxidant activity and antitumor effect. The results suggested that the novel polysaccharide WPP2 could poten- tially be used as a new natural medicine, but the relation of structure and functions need further study.

文章關(guān)鍵詞：

Keyword：:Pleurotus eryngii Polysaccharide Structure characterization Microscopic analysis Antitumoractivity

課題項目：國家自然科學(xué)基金（批準(zhǔn)號：10874108）和陜西省自然科學(xué)基礎(chǔ)研究計劃項目（批準(zhǔn)號：SJ08A16）資助.

作者信息：會員可見

  本文關(guān)鍵詞：杏鮑菇多糖WPP2的結(jié)構(gòu)表征及抗腫瘤活性，由筆耕文化傳播整理發(fā)布。