本文關(guān)鍵詞：天然產(chǎn)物抗氧化活性的常見評價方法，，由筆耕文化傳播整理發(fā)布。

摘要：

天然產(chǎn)物抗氧化活性的評價是天然產(chǎn)物研究的重要組成部分,對其在日化領(lǐng)域的開發(fā)應(yīng)用具有顯著的指導(dǎo)意義.本文從實驗原理、實驗方法等方面對天然產(chǎn)物抗氧化活性的常見評價方法進行綜述,詳細介紹了化學分析法中的自由基(ABTS自由基陽離子、DPPH自由基、超氧自由基、羥自由基)清除實驗、脂質(zhì)過氧化抑制實驗、還原能力測定及脂質(zhì)氧化抑制等評價方法;描述了以生物細胞為模型的CAA抗氧評價方法;概述了在動物體內(nèi)進行的抗氧化活性評價方法及指標,為相關(guān)領(lǐng)域中抗氧化天然產(chǎn)物的研究和開發(fā)提供實驗理論基礎(chǔ).

Abstract： 

Evaluation of antioxidant activity is important for the investigation of natural products,and is the useful guide for their application in chemical industry. In the present study,some typical analytical methods for evaluation of the antioxidant activity of natural products are reviewed in terms of their chemical mechanisms and common procedures. The in vitro methods,such as free radical (ABTS,DPPH,superoxide and hydroxyl free radical) scavenging,lipid peroxidation,reducing power and fat oxidation assays are introduced. Meanwhile,the cellular antioxidant activity (CAA) experiment based on determination of the vitality of cells is also described. Furthermore,the testing procedures and factors of in vivo method in animals are given. This review might be useful for the related research of natural products.

  本文關(guān)鍵詞：天然產(chǎn)物抗氧化活性的常見評價方法，由筆耕文化傳播整理發(fā)布。