目的基質(zhì)細(xì)胞之間或基質(zhì)細(xì)胞與癌細(xì)胞之間存在著細(xì)胞轉(zhuǎn)分化過程。吳茱萸堿和小檗堿是中藥方劑左金丸的主要藥理成分,二者在腫瘤微環(huán)境重塑中起著至關(guān)重要的作用。本研究旨在探討吳茱萸堿聯(lián)合小檗堿（cBerEvo）對腫瘤相關(guān)成纖維細(xì)胞誘導(dǎo)的結(jié)腸上皮細(xì)胞表型轉(zhuǎn)化的影響及其相關(guān)機(jī)制。方法采用制備好的人結(jié)腸肌成纖維細(xì)胞系CCD-18Co條件化培養(yǎng)基誘導(dǎo)正常人結(jié)腸上皮細(xì)胞系HCoEpiCs向間質(zhì)細(xì)胞轉(zhuǎn)化,同時觀察TGF-β受體抑制劑LY364947或cBerEvo對HCoEpiCs細(xì)胞轉(zhuǎn)化是否有抑制作用。用相差顯微鏡觀察HCoEpiCs形態(tài)學(xué)變化。免疫熒光顯微鏡觀察及檢測上皮細(xì)胞-間充質(zhì)轉(zhuǎn)化標(biāo)記物包括E-鈣粘蛋白（E-cadherin）,波形蛋白（vimentin）以及α-平滑肌肌動蛋白（α-SMA）的表達(dá)。創(chuàng)傷愈合試驗評估細(xì)胞遷移情況。Western blotting檢測E-cadherin、vimentin、α-SMA、Snail、ZEB1及Smads蛋白表達(dá)。結(jié)果與對照組細(xì)胞相比,經(jīng)腫瘤相關(guān)成纖維細(xì)胞樣CCD-18Co細(xì)胞誘導(dǎo)的HCoEpiCs細(xì)胞表達(dá)E-cadherin下調(diào)、表達(dá)vimentin、α... 

【文章來源】：Chinese Medical Sciences Journal. 2020,35(03)CSCD

【文章頁數(shù)】：13 頁

【文章目錄】：
MATERIALS AND METHODS
    Reagents and antibodies
    Cell culture and maintenance
    Preparation of CCD-18Co conditioned medium and induction
    Immunofluorescence microscopy
    Western blot analysis
    Wound healing assay
    Statistical analysis
RESULTS
    c Ber Evo inhibited the CCD-18Co-induced EMT-like morphological changes in colon epithelial cell HCo Epi Cs
    c B e r E v o m e d i a t e d t h e e x p r e s s i o n o f C C D-18Co-induced EMT biomarkers and relative transcription factors in HCo Epi Cs
    c Ber Evo repressed the CCD-18Co-induced migration of HCo Epi Cs
    c Ber Evo inactivated the CCD-18Co-induced TGF-β/Smads signaling in HCo Epi Cs
DISCUSSION
Conflict of Interests Statement



本文編號：3557323