四君子湯對(duì)16月齡小鼠血漿內(nèi)源性代謝物質(zhì)的影響
發(fā)布時(shí)間：2021-08-04 03:07
目的:基于代謝組學(xué)方法觀察四君子湯對(duì)16月齡小鼠血漿代謝影響,解析四君子湯對(duì)小鼠增齡過(guò)程的作用。方法:基于UPLC-HDMS技術(shù),采用主成分（PCA）和正交偏最小二乘-判別分析法（OPLS-DA）分析3月齡對(duì)照組與16月齡空白組、16月齡給藥組小鼠血漿代謝物及其所參與的代謝通路,探討標(biāo)志物及代謝通路的增齡性變化。結(jié)果:當(dāng)取VIP>1時(shí),篩查得到37個(gè)與衰老相關(guān)的潛在的生物標(biāo)記物,四君子湯對(duì)16個(gè)代謝標(biāo)志物起到了調(diào)節(jié)作用,其中14個(gè)具有統(tǒng)計(jì)學(xué)意義,它們參與了8條代謝途徑;當(dāng)取VIP>2時(shí),篩查得到15個(gè)與衰老相關(guān)的潛在的生物標(biāo)記物,四君子湯對(duì)3個(gè)代謝標(biāo)志物起到了調(diào)節(jié)作用,其中3個(gè)具有統(tǒng)計(jì)學(xué)意義,它們參與了1條代謝途徑。結(jié)論:四君子湯可以通過(guò)介入酪氨酸代謝、色氨酸代謝、不飽和脂肪酸的生物合成影響生物體的生長(zhǎng)進(jìn)程,為四君子湯干預(yù)小鼠增齡過(guò)程的生物學(xué)基礎(chǔ)提供依據(jù)。
【文章來(lái)源】：中醫(yī)藥學(xué)報(bào). 2020,48(05)
【文章頁(yè)數(shù)】：7 頁(yè)
【部分圖文】：
3月齡對(duì)照組、16月齡空白組及16月齡給藥組小鼠血液樣品PCA模式識(shí)別分析Score plot圖
圖1 3月齡對(duì)照組、16月齡空白組及16月齡給藥組小鼠血液樣品PCA模式識(shí)別分析Score plot圖表4 正負(fù)離子條件下16月雌鼠血液樣品生物標(biāo)志物(VIP>1) 化合物 加合物 公式 物質(zhì)名稱 方差分析 VIP值 作用趨勢(shì) HMDB00929 M+FA-H, 2M+FA-H, 2M-H, M-H C11H12N2O2 L-Tryptophan 0.000 606 28 1.648 23 上 HMDB00682* M-H, 2M-H C8H7NO4S Indoxyl sulfate 0.007 716 15 2.248 81 上 HMDB00307 M+FA-H, 2M-H C24H40O6 1b-Hydroxycholic acid 4.28E-08 1.387 上 HMDB00348* M+FA-H, 2M-H, M-H C24H40O4 3b,12a-Dihydroxy-5a-cholanoic acid 2.96E-07 2.342 58 上 HMDB00896 2M+FA-H C26H45NO6S Taurodeoxycholic acid 0.000 707 89 1.138 29 上 HMDB04866 M+FA-H C42H79NO13 Lactosylceramide(d18:1/12:0) 7.89E-05 1.090 33 上 HMDB02486 M-H C26H45NO9S2 Taurochenodeoxycholate-3-sulfate 4.70E-06 1.218 22 下 HMDB10361 M+FA-H C33H50O10 (23S)-23,25-dihdroxy-24-oxovitamine D3 23-(beta-glucuronide) 1.89E-07 1.026 73 上 HMDB04667 M-H, M+FA-H C18H32O3 13S-hydroxyoctadecadienoic acid 0.001 620 01 1.180 46 下 HMDB60352* 2M+FA-H C18H24O4 2-Polyprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone 7.92E-08 2.318 85 上 HMDB00145 2M+FA-H C18H22O2 Estrone 8.60E-06 1.820 99 上 HMDB02429 M+FA-H C32H53NO11S (3a,5b)-24-oxo-24-[(2-sulfoethyl)amino]cholan-3-yl-b-D-Glucopyranosiduronic acid 8.29E-07 1.364 9 上 HMDB59990 2M+FA-H C18H22O10 5-(3",4"-Dihydroxyphenyl)-gamma-valerolactone-4"-O-methyl-3"-O-glucuronide 7.29E-08 1.01665 下 HMDB06772 2M-H C19H24O3 Adrenosterone 0.001 351 03 1.7992 9 上 HMDB02183 M+FA-H, 2M-H, M-H C22H32O2 Docosahexaenoic acid 0.009 960 58 1.119 89 上 HMDB01060 M+FA-H C49H78O4 Ubiquinol 8 0.000 837 16 1.235 94 上 HMDB03349 M-H, M+FA-H C5H6N2O4 L-Dihydroorotic acid 2.11E-12 1.825 78 上 HMDB01896* 2M-H C11H13NO2 5-Methoxytryptophol 1.50E-08 2.064 2 上 HMDB01586* M+H-H2O C6H13O9P Glucose 1-phosphate 1.60E-11 2.51964 下 HMDB00172* M+H,M+Na C6H13NO2 L-Isoleucine 1.31E-07 2.2216 下 HMDB01551 M+NH4 C16H32O Palmitaldehyde 2.57E-07 1.309 62 上 HMDB00638 2M+NH4 C12H24O2 Dodecanoic acid 3.64E-08 1.155 47 上 HMDB02384 M+NH4 C18H36O Stearaldehyde 2.72E-08 1.256 98 上 HMDB00086 M+H-H2O C8H20NO6P Glycerophosphocholine 0.001 110 75 1.062 94 上 HMDB12562 M+NH4,M+Na, M+H C28H42O3 13"-Hydroxy-gamma-tocotrienol 0.000 187 7 1.078 09 下 HMDB04985* M+NH4, 2M+NH4 C10H19N3O5 Aspartylysine 2.02E-10 2.344 93 下 HMDB13154 M+H-H2O C25H49NO5 12-Hydroxy-12-octadecanoylcarnitine 0.003 630 66 1.135 37 上 HMDB05874 M+Na C9H10BrNO3 3-Bromotyrosine 1.95E-07 1.285 19 下 HMDB00944* M+NH4 C22H44O2 Behenic acid 2.37E-08 2.488 93 上 HMDB01058* M+H-H2O C6H14O12P2 Fructose 1,6-bisphosphate 7.12E-10 2.055 09 下 HMDB01268* M+H-H2O C8H8O6 4-Fumarylacetoacetic acid 3.81E-09 3.298 18 上 HMDB06852* M+NH4 C29H46O 5-Dehydroavenasterol 2.22E-16 3.497 63 下 HMDB01455* 2M+NH4 C21H34O2 Alloepipregnanolone 5.33E-15 2.126 12 上 HMDB04610* M+Na C18H39NO3 Phytosphingosine 0 2.098 23 下 HMDB12321 M+NH4 C38H73NO8 Galactosylceramide (d18:1/14:0) 6.03E-09 1.372 08 上 HMDB00182* M+NH4, 2M+NH4 C6H14N2O2 L-Lysine 0 7.132 21 下 HMDB15517* M+H, M+NH4 C10H15N Methamphetamine 0 12.73 49 下 注:*表示該物質(zhì)VIP值大于2
表4 正負(fù)離子條件下16月雌鼠血液樣品生物標(biāo)志物(VIP>1) 化合物 加合物 公式 物質(zhì)名稱 方差分析 VIP值 作用趨勢(shì) HMDB00929 M+FA-H, 2M+FA-H, 2M-H, M-H C11H12N2O2 L-Tryptophan 0.000 606 28 1.648 23 上 HMDB00682* M-H, 2M-H C8H7NO4S Indoxyl sulfate 0.007 716 15 2.248 81 上 HMDB00307 M+FA-H, 2M-H C24H40O6 1b-Hydroxycholic acid 4.28E-08 1.387 上 HMDB00348* M+FA-H, 2M-H, M-H C24H40O4 3b,12a-Dihydroxy-5a-cholanoic acid 2.96E-07 2.342 58 上 HMDB00896 2M+FA-H C26H45NO6S Taurodeoxycholic acid 0.000 707 89 1.138 29 上 HMDB04866 M+FA-H C42H79NO13 Lactosylceramide(d18:1/12:0) 7.89E-05 1.090 33 上 HMDB02486 M-H C26H45NO9S2 Taurochenodeoxycholate-3-sulfate 4.70E-06 1.218 22 下 HMDB10361 M+FA-H C33H50O10 (23S)-23,25-dihdroxy-24-oxovitamine D3 23-(beta-glucuronide) 1.89E-07 1.026 73 上 HMDB04667 M-H, M+FA-H C18H32O3 13S-hydroxyoctadecadienoic acid 0.001 620 01 1.180 46 下 HMDB60352* 2M+FA-H C18H24O4 2-Polyprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone 7.92E-08 2.318 85 上 HMDB00145 2M+FA-H C18H22O2 Estrone 8.60E-06 1.820 99 上 HMDB02429 M+FA-H C32H53NO11S (3a,5b)-24-oxo-24-[(2-sulfoethyl)amino]cholan-3-yl-b-D-Glucopyranosiduronic acid 8.29E-07 1.364 9 上 HMDB59990 2M+FA-H C18H22O10 5-(3",4"-Dihydroxyphenyl)-gamma-valerolactone-4"-O-methyl-3"-O-glucuronide 7.29E-08 1.01665 下 HMDB06772 2M-H C19H24O3 Adrenosterone 0.001 351 03 1.7992 9 上 HMDB02183 M+FA-H, 2M-H, M-H C22H32O2 Docosahexaenoic acid 0.009 960 58 1.119 89 上 HMDB01060 M+FA-H C49H78O4 Ubiquinol 8 0.000 837 16 1.235 94 上 HMDB03349 M-H, M+FA-H C5H6N2O4 L-Dihydroorotic acid 2.11E-12 1.825 78 上 HMDB01896* 2M-H C11H13NO2 5-Methoxytryptophol 1.50E-08 2.064 2 上 HMDB01586* M+H-H2O C6H13O9P Glucose 1-phosphate 1.60E-11 2.51964 下 HMDB00172* M+H,M+Na C6H13NO2 L-Isoleucine 1.31E-07 2.2216 下 HMDB01551 M+NH4 C16H32O Palmitaldehyde 2.57E-07 1.309 62 上 HMDB00638 2M+NH4 C12H24O2 Dodecanoic acid 3.64E-08 1.155 47 上 HMDB02384 M+NH4 C18H36O Stearaldehyde 2.72E-08 1.256 98 上 HMDB00086 M+H-H2O C8H20NO6P Glycerophosphocholine 0.001 110 75 1.062 94 上 HMDB12562 M+NH4,M+Na, M+H C28H42O3 13"-Hydroxy-gamma-tocotrienol 0.000 187 7 1.078 09 下 HMDB04985* M+NH4, 2M+NH4 C10H19N3O5 Aspartylysine 2.02E-10 2.344 93 下 HMDB13154 M+H-H2O C25H49NO5 12-Hydroxy-12-octadecanoylcarnitine 0.003 630 66 1.135 37 上 HMDB05874 M+Na C9H10BrNO3 3-Bromotyrosine 1.95E-07 1.285 19 下 HMDB00944* M+NH4 C22H44O2 Behenic acid 2.37E-08 2.488 93 上 HMDB01058* M+H-H2O C6H14O12P2 Fructose 1,6-bisphosphate 7.12E-10 2.055 09 下 HMDB01268* M+H-H2O C8H8O6 4-Fumarylacetoacetic acid 3.81E-09 3.298 18 上 HMDB06852* M+NH4 C29H46O 5-Dehydroavenasterol 2.22E-16 3.497 63 下 HMDB01455* 2M+NH4 C21H34O2 Alloepipregnanolone 5.33E-15 2.126 12 上 HMDB04610* M+Na C18H39NO3 Phytosphingosine 0 2.098 23 下 HMDB12321 M+NH4 C38H73NO8 Galactosylceramide (d18:1/14:0) 6.03E-09 1.372 08 上 HMDB00182* M+NH4, 2M+NH4 C6H14N2O2 L-Lysine 0 7.132 21 下 HMDB15517* M+H, M+NH4 C10H15N Methamphetamine 0 12.73 49 下 注:*表示該物質(zhì)VIP值大于2圖4 16月齡空白組、16月齡給藥組小鼠血液樣品OPLS-DA模式識(shí)別分析后S-Plot圖
本文編號(hào)：3320877
【文章來(lái)源】：中醫(yī)藥學(xué)報(bào). 2020,48(05)
【文章頁(yè)數(shù)】：7 頁(yè)
【部分圖文】：
3月齡對(duì)照組、16月齡空白組及16月齡給藥組小鼠血液樣品PCA模式識(shí)別分析Score plot圖
圖1 3月齡對(duì)照組、16月齡空白組及16月齡給藥組小鼠血液樣品PCA模式識(shí)別分析Score plot圖表4 正負(fù)離子條件下16月雌鼠血液樣品生物標(biāo)志物(VIP>1) 化合物 加合物 公式 物質(zhì)名稱 方差分析 VIP值 作用趨勢(shì) HMDB00929 M+FA-H, 2M+FA-H, 2M-H, M-H C11H12N2O2 L-Tryptophan 0.000 606 28 1.648 23 上 HMDB00682* M-H, 2M-H C8H7NO4S Indoxyl sulfate 0.007 716 15 2.248 81 上 HMDB00307 M+FA-H, 2M-H C24H40O6 1b-Hydroxycholic acid 4.28E-08 1.387 上 HMDB00348* M+FA-H, 2M-H, M-H C24H40O4 3b,12a-Dihydroxy-5a-cholanoic acid 2.96E-07 2.342 58 上 HMDB00896 2M+FA-H C26H45NO6S Taurodeoxycholic acid 0.000 707 89 1.138 29 上 HMDB04866 M+FA-H C42H79NO13 Lactosylceramide(d18:1/12:0) 7.89E-05 1.090 33 上 HMDB02486 M-H C26H45NO9S2 Taurochenodeoxycholate-3-sulfate 4.70E-06 1.218 22 下 HMDB10361 M+FA-H C33H50O10 (23S)-23,25-dihdroxy-24-oxovitamine D3 23-(beta-glucuronide) 1.89E-07 1.026 73 上 HMDB04667 M-H, M+FA-H C18H32O3 13S-hydroxyoctadecadienoic acid 0.001 620 01 1.180 46 下 HMDB60352* 2M+FA-H C18H24O4 2-Polyprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone 7.92E-08 2.318 85 上 HMDB00145 2M+FA-H C18H22O2 Estrone 8.60E-06 1.820 99 上 HMDB02429 M+FA-H C32H53NO11S (3a,5b)-24-oxo-24-[(2-sulfoethyl)amino]cholan-3-yl-b-D-Glucopyranosiduronic acid 8.29E-07 1.364 9 上 HMDB59990 2M+FA-H C18H22O10 5-(3",4"-Dihydroxyphenyl)-gamma-valerolactone-4"-O-methyl-3"-O-glucuronide 7.29E-08 1.01665 下 HMDB06772 2M-H C19H24O3 Adrenosterone 0.001 351 03 1.7992 9 上 HMDB02183 M+FA-H, 2M-H, M-H C22H32O2 Docosahexaenoic acid 0.009 960 58 1.119 89 上 HMDB01060 M+FA-H C49H78O4 Ubiquinol 8 0.000 837 16 1.235 94 上 HMDB03349 M-H, M+FA-H C5H6N2O4 L-Dihydroorotic acid 2.11E-12 1.825 78 上 HMDB01896* 2M-H C11H13NO2 5-Methoxytryptophol 1.50E-08 2.064 2 上 HMDB01586* M+H-H2O C6H13O9P Glucose 1-phosphate 1.60E-11 2.51964 下 HMDB00172* M+H,M+Na C6H13NO2 L-Isoleucine 1.31E-07 2.2216 下 HMDB01551 M+NH4 C16H32O Palmitaldehyde 2.57E-07 1.309 62 上 HMDB00638 2M+NH4 C12H24O2 Dodecanoic acid 3.64E-08 1.155 47 上 HMDB02384 M+NH4 C18H36O Stearaldehyde 2.72E-08 1.256 98 上 HMDB00086 M+H-H2O C8H20NO6P Glycerophosphocholine 0.001 110 75 1.062 94 上 HMDB12562 M+NH4,M+Na, M+H C28H42O3 13"-Hydroxy-gamma-tocotrienol 0.000 187 7 1.078 09 下 HMDB04985* M+NH4, 2M+NH4 C10H19N3O5 Aspartylysine 2.02E-10 2.344 93 下 HMDB13154 M+H-H2O C25H49NO5 12-Hydroxy-12-octadecanoylcarnitine 0.003 630 66 1.135 37 上 HMDB05874 M+Na C9H10BrNO3 3-Bromotyrosine 1.95E-07 1.285 19 下 HMDB00944* M+NH4 C22H44O2 Behenic acid 2.37E-08 2.488 93 上 HMDB01058* M+H-H2O C6H14O12P2 Fructose 1,6-bisphosphate 7.12E-10 2.055 09 下 HMDB01268* M+H-H2O C8H8O6 4-Fumarylacetoacetic acid 3.81E-09 3.298 18 上 HMDB06852* M+NH4 C29H46O 5-Dehydroavenasterol 2.22E-16 3.497 63 下 HMDB01455* 2M+NH4 C21H34O2 Alloepipregnanolone 5.33E-15 2.126 12 上 HMDB04610* M+Na C18H39NO3 Phytosphingosine 0 2.098 23 下 HMDB12321 M+NH4 C38H73NO8 Galactosylceramide (d18:1/14:0) 6.03E-09 1.372 08 上 HMDB00182* M+NH4, 2M+NH4 C6H14N2O2 L-Lysine 0 7.132 21 下 HMDB15517* M+H, M+NH4 C10H15N Methamphetamine 0 12.73 49 下 注:*表示該物質(zhì)VIP值大于2
表4 正負(fù)離子條件下16月雌鼠血液樣品生物標(biāo)志物(VIP>1) 化合物 加合物 公式 物質(zhì)名稱 方差分析 VIP值 作用趨勢(shì) HMDB00929 M+FA-H, 2M+FA-H, 2M-H, M-H C11H12N2O2 L-Tryptophan 0.000 606 28 1.648 23 上 HMDB00682* M-H, 2M-H C8H7NO4S Indoxyl sulfate 0.007 716 15 2.248 81 上 HMDB00307 M+FA-H, 2M-H C24H40O6 1b-Hydroxycholic acid 4.28E-08 1.387 上 HMDB00348* M+FA-H, 2M-H, M-H C24H40O4 3b,12a-Dihydroxy-5a-cholanoic acid 2.96E-07 2.342 58 上 HMDB00896 2M+FA-H C26H45NO6S Taurodeoxycholic acid 0.000 707 89 1.138 29 上 HMDB04866 M+FA-H C42H79NO13 Lactosylceramide(d18:1/12:0) 7.89E-05 1.090 33 上 HMDB02486 M-H C26H45NO9S2 Taurochenodeoxycholate-3-sulfate 4.70E-06 1.218 22 下 HMDB10361 M+FA-H C33H50O10 (23S)-23,25-dihdroxy-24-oxovitamine D3 23-(beta-glucuronide) 1.89E-07 1.026 73 上 HMDB04667 M-H, M+FA-H C18H32O3 13S-hydroxyoctadecadienoic acid 0.001 620 01 1.180 46 下 HMDB60352* 2M+FA-H C18H24O4 2-Polyprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone 7.92E-08 2.318 85 上 HMDB00145 2M+FA-H C18H22O2 Estrone 8.60E-06 1.820 99 上 HMDB02429 M+FA-H C32H53NO11S (3a,5b)-24-oxo-24-[(2-sulfoethyl)amino]cholan-3-yl-b-D-Glucopyranosiduronic acid 8.29E-07 1.364 9 上 HMDB59990 2M+FA-H C18H22O10 5-(3",4"-Dihydroxyphenyl)-gamma-valerolactone-4"-O-methyl-3"-O-glucuronide 7.29E-08 1.01665 下 HMDB06772 2M-H C19H24O3 Adrenosterone 0.001 351 03 1.7992 9 上 HMDB02183 M+FA-H, 2M-H, M-H C22H32O2 Docosahexaenoic acid 0.009 960 58 1.119 89 上 HMDB01060 M+FA-H C49H78O4 Ubiquinol 8 0.000 837 16 1.235 94 上 HMDB03349 M-H, M+FA-H C5H6N2O4 L-Dihydroorotic acid 2.11E-12 1.825 78 上 HMDB01896* 2M-H C11H13NO2 5-Methoxytryptophol 1.50E-08 2.064 2 上 HMDB01586* M+H-H2O C6H13O9P Glucose 1-phosphate 1.60E-11 2.51964 下 HMDB00172* M+H,M+Na C6H13NO2 L-Isoleucine 1.31E-07 2.2216 下 HMDB01551 M+NH4 C16H32O Palmitaldehyde 2.57E-07 1.309 62 上 HMDB00638 2M+NH4 C12H24O2 Dodecanoic acid 3.64E-08 1.155 47 上 HMDB02384 M+NH4 C18H36O Stearaldehyde 2.72E-08 1.256 98 上 HMDB00086 M+H-H2O C8H20NO6P Glycerophosphocholine 0.001 110 75 1.062 94 上 HMDB12562 M+NH4,M+Na, M+H C28H42O3 13"-Hydroxy-gamma-tocotrienol 0.000 187 7 1.078 09 下 HMDB04985* M+NH4, 2M+NH4 C10H19N3O5 Aspartylysine 2.02E-10 2.344 93 下 HMDB13154 M+H-H2O C25H49NO5 12-Hydroxy-12-octadecanoylcarnitine 0.003 630 66 1.135 37 上 HMDB05874 M+Na C9H10BrNO3 3-Bromotyrosine 1.95E-07 1.285 19 下 HMDB00944* M+NH4 C22H44O2 Behenic acid 2.37E-08 2.488 93 上 HMDB01058* M+H-H2O C6H14O12P2 Fructose 1,6-bisphosphate 7.12E-10 2.055 09 下 HMDB01268* M+H-H2O C8H8O6 4-Fumarylacetoacetic acid 3.81E-09 3.298 18 上 HMDB06852* M+NH4 C29H46O 5-Dehydroavenasterol 2.22E-16 3.497 63 下 HMDB01455* 2M+NH4 C21H34O2 Alloepipregnanolone 5.33E-15 2.126 12 上 HMDB04610* M+Na C18H39NO3 Phytosphingosine 0 2.098 23 下 HMDB12321 M+NH4 C38H73NO8 Galactosylceramide (d18:1/14:0) 6.03E-09 1.372 08 上 HMDB00182* M+NH4, 2M+NH4 C6H14N2O2 L-Lysine 0 7.132 21 下 HMDB15517* M+H, M+NH4 C10H15N Methamphetamine 0 12.73 49 下 注:*表示該物質(zhì)VIP值大于2圖4 16月齡空白組、16月齡給藥組小鼠血液樣品OPLS-DA模式識(shí)別分析后S-Plot圖
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