目的探討Wnt/β-catennin信號通路介導(dǎo)的淫羊藿苷（Icariin）調(diào)控BMSCs（SD大鼠）成骨-成脂雙向分化。方法1.體外實驗（1）用細胞增殖、毒性試劑盒（CCK-8）檢測BMSCs在多種不同濃度Icariin的干預(yù)其增殖活性的強弱及是細胞產(chǎn)生毒性反應(yīng)。（2）用RT-q PCR確定Icariin對成骨分化指標（ALP、COL1、RUNX2）的影響,使用堿性磷酸酶（ALP）染色試劑盒和茜素紅染色試劑確定經(jīng)Icariin干預(yù)后ALP的活性及強弱、是否鈣結(jié)節(jié)的形成及形成量（3）用RT-q PCR確定Icariin對成脂分化指標（PPARγ、Adipsin、FABP4）的影響,使用油紅O染色試劑確定脂滴的形成。（4）使用信號通路特異性抑制蛋白DKK-1聯(lián)合Icariin進行干預(yù),檢測Icariin在BMSCs成骨-成脂分化的作用。（5）Western-blot及Immunofluorescence檢測Icariin干預(yù)后Wnt信號通路關(guān)鍵蛋白P-GSK、β-catennin表達和定位情況。2.在體試驗用12周齡C57小鼠做卵巢切除模擬絕經(jīng)后骨質(zhì)疏松（OVX）,口服Icariin（2... 

【文章來源】：華中科技大學(xué)湖北省 211工程院校 985工程院校 教育部直屬院校

【文章頁數(shù)】：69 頁

【學(xué)位級別】：碩士

【文章目錄】：
前言
    參考文獻
中文摘要
Abstract
引言
材料與方法
結(jié)果
討論
參考文獻
綜述
    參考文獻
附錄一
附錄二 研究生期間發(fā)表的論文
致謝


【參考文獻】：
期刊論文
[1]暗紫貝母水溶性成分HPLC指紋圖譜研究[J]. 段寶忠,陳士林,魏大華,黃林芳,王珍.  中國中藥雜志. 2012(13)
[2]Icariine stimulates proliferation and differentiation of human osteoblasts by increasing production of bone morphogenetic protein 2[J]. YIN Xiao-xue CHEN Zhong-qiang LIU Zhong-jun MA Qing-jun DANG Geng-ting Department of Orthopaedics,Peking University Third Hospital,Beijing,100083,China (Yin XX,Chen ZQ,Liu ZJ,Ma QJ and Dang GJ).  Chinese Medical Journal. 2007(03)
[3]The testosterone mimetic properties of icariin[J]. Zhen-Bao Zhang Qing-Tao Yang Department of Urology,Second Affiliated Hospital,Shantou University Medical College,Shantou 515041,China.  Asian Journal of Andrology. 2006(05)
[4]Icariin-mediated expression of cardiac genes and modulation of nitric oxide signaling pathway during differentiation of mouse embryonic stem cells into cardiomyocytes in vitro[J]. Dan-yan ZHU Yi-jia LOU~2 Department of Pharmacology and Toxicology.College of Pharmaceutical Sciences.Zhejiang University,Hangzhou 310031.China.  Acta Pharmacologica Sinica. 2006(03)
[5]Effects of icariin on erectile function and expression of nitric oxide synthase isoforms in castrated rats[J]. Wu-Jiang Liu Zhong-Cheng Xin Hua Xin Yi-Ming Yuan Long Tian Ying-Lu Guo Andrology Center of Peking University First Hospital,Beijing 100009,China.  Asian Journal of Andrology. 2005(04)
[6]Protective effects of icariin on neurons injured by cerebral ischemia/reperfusion[J]. LI Li, ZHOU Qi-xin and SHI Jing-shanDepartment of Pharmacology, Zunyi Medical College, Zunyi 563003, ChinaDepartment of Pharmacology,Chongqing Medical University, Chongqing400016,China.  Chinese Medical Journal. 2005(19)



本文編號：3217968