目的:觀察白藜蘆醇對(duì)急性T淋巴細(xì)胞白血病小鼠的作用及Notch1、Hes-1信號(hào)分子的表達(dá)水平,并探討其作用機(jī)制。方法:1.完全隨機(jī)法將50只C57BL/6雌性健康小鼠分為5組:正常對(duì)照組;模型對(duì)照組;白藜蘆醇低濃度處理組（25mg/kg/d）;白藜蘆醇中濃度處理組（50mg/kg/d）;白藜蘆醇高濃度處理組（100mg/kg/d）。尾靜脈注射1×10⁶個(gè)GFP⁺白血病細(xì)胞,制備T-ALL白血病小鼠模型。2.應(yīng)用瑞氏-吉姆薩染色觀察各組外周血白血病細(xì)胞浸潤情況。3.流式細(xì)胞術(shù)檢測(cè)各實(shí)驗(yàn)組外周血、脾細(xì)胞懸液中白血病細(xì)胞的百分比。4.HE染色法觀察各實(shí)驗(yàn)組肝臟、脾臟、腎臟、骨髓組織中白血病細(xì)胞的浸潤情況。5.應(yīng)用實(shí)時(shí)熒光定量PCR（RT-PCR）檢測(cè)各組脾臟組織中Notch1、Hes-1信號(hào)分子mRNA的表達(dá)量。6.應(yīng)用免疫蛋白印跡法（Western Blot）檢測(cè)各組脾臟組織中Notch1、Hes-1蛋白表達(dá)水平。結(jié)果:1.模型對(duì)照組、白藜蘆醇低、中、高濃度處理組的外周血涂片均可觀察到原始淋巴細(xì)胞,其細(xì)胞核呈圓形或略呈卵圓形,大而深染,染色質(zhì)致... 

【文章來源】：河北醫(yī)科大學(xué)河北省

【文章頁數(shù)】：44 頁

【學(xué)位級(jí)別】：碩士

【部分圖文】：

57BL/6小鼠與T-ALL模型小鼠的一般情況

23影響Fig.2 Effect of resveratrol on leukemia cells of peripheral blood in micewith T-ALL by FCM.A: Original recordings of FCM. P2: The rates of GFP+cells.B: Comparison of GFP+cell rates among different groups at different times.a: control group; b: model group; c: 25mg/kg/d Res group; d: 50 mg/kg/d Res

圖 3 流式細(xì)胞術(shù)檢測(cè)白藜蘆醇對(duì) T-ALL 白血病小鼠脾細(xì)胞懸液中白血病細(xì)胞的影響Fig.3 Effect of resveratrol on leukemia cells of spleen cell suspension in micewith T-ALL by FCM.All data were expressed as mean ± SD; n=5 for each group.#P﹤0.05 vs.model group.AB

【參考文獻(xiàn)】：
期刊論文
[1]虎杖提取物白藜蘆醇抗腫瘤機(jī)制的研究進(jìn)展[J]. 何蕾,倪敏,樊志敏.  中醫(yī)藥導(dǎo)報(bào). 2017(08)
[2]白藜蘆醇逆轉(zhuǎn)腫瘤耐藥的分子機(jī)制研究進(jìn)展[J]. 郭玉楷,史敏,秦永亮,李永軍.  基礎(chǔ)醫(yī)學(xué)與臨床. 2016(03)
[3]Notch1信號(hào)通路與白藜蘆醇抗急性T淋巴細(xì)胞白血病作用機(jī)制研究進(jìn)展[J]. 李永軍,史敏,秦永亮.  臨床檢驗(yàn)雜志. 2012(10)
[4]Effect of resveratrol and in combination with 5-FU on murine liver cancer[J]. Sheng-Li Wu Zhong-Jie Sun Liang Yu Ke-Wei Meng Xing-Lei Qin Cheng-En Pan Department of Hepatobiliary Surgery,First Hospital of Xi’an Jiaotong University,Xi’an 710061,Shaanxi Province,ChinaDepartment of Hepatobiliary Surgery,People’s Hospital of Shaanxi Province,Xi’an 710061,Shaanxi Province,China.  World Journal of Gastroenterology. 2004(20)
[5]Effect of resveratrol on cell cycle proteins in murine transplantable liver cancer[J]. Liang Yu Zhong-Jie Sun Sheng-Li Wu Cheng-En Pan, Department of Hepatobiliary Surgery, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China.  World Journal of Gastroenterology. 2003(10)



本文編號(hào)：3130761