白藜蘆醇抗小鼠急性T淋巴細胞白血病作用及Notch1信號通路機制的研究
發(fā)布時間：2021-04-11 06:30
目的:觀察白藜蘆醇對急性T淋巴細胞白血病小鼠的作用及Notch1、Hes-1信號分子的表達水平,并探討其作用機制。方法:1.完全隨機法將50只C57BL/6雌性健康小鼠分為5組:正常對照組;模型對照組;白藜蘆醇低濃度處理組（25mg/kg/d）;白藜蘆醇中濃度處理組（50mg/kg/d）;白藜蘆醇高濃度處理組（100mg/kg/d）。尾靜脈注射1×106個GFP+白血病細胞,制備T-ALL白血病小鼠模型。2.應用瑞氏-吉姆薩染色觀察各組外周血白血病細胞浸潤情況。3.流式細胞術檢測各實驗組外周血、脾細胞懸液中白血病細胞的百分比。4.HE染色法觀察各實驗組肝臟、脾臟、腎臟、骨髓組織中白血病細胞的浸潤情況。5.應用實時熒光定量PCR（RT-PCR）檢測各組脾臟組織中Notch1、Hes-1信號分子mRNA的表達量。6.應用免疫蛋白印跡法（Western Blot）檢測各組脾臟組織中Notch1、Hes-1蛋白表達水平。結(jié)果:1.模型對照組、白藜蘆醇低、中、高濃度處理組的外周血涂片均可觀察到原始淋巴細胞,其細胞核呈圓形或略呈卵圓形,大而深染,染色質(zhì)致...
【文章來源】：河北醫(yī)科大學河北省
【文章頁數(shù)】：44 頁
【學位級別】：碩士
【部分圖文】：
57BL/6小鼠與T-ALL模型小鼠的一般情況
23影響Fig.2 Effect of resveratrol on leukemia cells of peripheral blood in micewith T-ALL by FCM.A: Original recordings of FCM. P2: The rates of GFP+cells.B: Comparison of GFP+cell rates among different groups at different times.a: control group; b: model group; c: 25mg/kg/d Res group; d: 50 mg/kg/d Res
圖 3 流式細胞術檢測白藜蘆醇對 T-ALL 白血病小鼠脾細胞懸液中白血病細胞的影響Fig.3 Effect of resveratrol on leukemia cells of spleen cell suspension in micewith T-ALL by FCM.All data were expressed as mean ± SD; n=5 for each group.#P﹤0.05 vs.model group.AB
【參考文獻】：
期刊論文
[1]虎杖提取物白藜蘆醇抗腫瘤機制的研究進展[J]. 何蕾,倪敏,樊志敏. 中醫(yī)藥導報. 2017(08)
[2]白藜蘆醇逆轉(zhuǎn)腫瘤耐藥的分子機制研究進展[J]. 郭玉楷,史敏,秦永亮,李永軍. 基礎醫(yī)學與臨床. 2016(03)
[3]Notch1信號通路與白藜蘆醇抗急性T淋巴細胞白血病作用機制研究進展[J]. 李永軍,史敏,秦永亮. 臨床檢驗雜志. 2012(10)
[4]Effect of resveratrol and in combination with 5-FU on murine liver cancer[J]. Sheng-Li Wu Zhong-Jie Sun Liang Yu Ke-Wei Meng Xing-Lei Qin Cheng-En Pan Department of Hepatobiliary Surgery,First Hospital of Xi’an Jiaotong University,Xi’an 710061,Shaanxi Province,ChinaDepartment of Hepatobiliary Surgery,People’s Hospital of Shaanxi Province,Xi’an 710061,Shaanxi Province,China. World Journal of Gastroenterology. 2004(20)
[5]Effect of resveratrol on cell cycle proteins in murine transplantable liver cancer[J]. Liang Yu Zhong-Jie Sun Sheng-Li Wu Cheng-En Pan, Department of Hepatobiliary Surgery, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China. World Journal of Gastroenterology. 2003(10)
本文編號：3130761
【文章來源】：河北醫(yī)科大學河北省
【文章頁數(shù)】：44 頁
【學位級別】：碩士
【部分圖文】：
57BL/6小鼠與T-ALL模型小鼠的一般情況
23影響Fig.2 Effect of resveratrol on leukemia cells of peripheral blood in micewith T-ALL by FCM.A: Original recordings of FCM. P2: The rates of GFP+cells.B: Comparison of GFP+cell rates among different groups at different times.a: control group; b: model group; c: 25mg/kg/d Res group; d: 50 mg/kg/d Res
圖 3 流式細胞術檢測白藜蘆醇對 T-ALL 白血病小鼠脾細胞懸液中白血病細胞的影響Fig.3 Effect of resveratrol on leukemia cells of spleen cell suspension in micewith T-ALL by FCM.All data were expressed as mean ± SD; n=5 for each group.#P﹤0.05 vs.model group.AB
【參考文獻】：
期刊論文
[1]虎杖提取物白藜蘆醇抗腫瘤機制的研究進展[J]. 何蕾,倪敏,樊志敏. 中醫(yī)藥導報. 2017(08)
[2]白藜蘆醇逆轉(zhuǎn)腫瘤耐藥的分子機制研究進展[J]. 郭玉楷,史敏,秦永亮,李永軍. 基礎醫(yī)學與臨床. 2016(03)
[3]Notch1信號通路與白藜蘆醇抗急性T淋巴細胞白血病作用機制研究進展[J]. 李永軍,史敏,秦永亮. 臨床檢驗雜志. 2012(10)
[4]Effect of resveratrol and in combination with 5-FU on murine liver cancer[J]. Sheng-Li Wu Zhong-Jie Sun Liang Yu Ke-Wei Meng Xing-Lei Qin Cheng-En Pan Department of Hepatobiliary Surgery,First Hospital of Xi’an Jiaotong University,Xi’an 710061,Shaanxi Province,ChinaDepartment of Hepatobiliary Surgery,People’s Hospital of Shaanxi Province,Xi’an 710061,Shaanxi Province,China. World Journal of Gastroenterology. 2004(20)
[5]Effect of resveratrol on cell cycle proteins in murine transplantable liver cancer[J]. Liang Yu Zhong-Jie Sun Sheng-Li Wu Cheng-En Pan, Department of Hepatobiliary Surgery, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China. World Journal of Gastroenterology. 2003(10)
本文編號：3130761
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