【摘要】：目的探討海水淹溺型急性肺損傷(SWD-ALI)時(shí)外周血中性粒細(xì)胞(PMN)凋亡的演變規(guī)律以及細(xì)胞因子對(duì)其可能的影響。方法36只新西蘭兔用隨機(jī)數(shù)字表法均分為對(duì)照組(作為0h基線值)和海水灌注后1、3、6、12、24h組(分別于灌注后1、3、6、12、24h處死)。觀察各組血象和血?dú)夥治鲋笜?biāo)的變化,計(jì)算肺濕/干重比(W/D)和肺微血管通透指數(shù);流式細(xì)胞術(shù)檢測(cè)PMN凋亡情況,ELISA法檢測(cè)血清TNF-α、IL-1β、IL-10水平;對(duì)肺組織進(jìn)行病理學(xué)觀察,并計(jì)算病理評(píng)分(LPS)。結(jié)果W/D值在3h達(dá)高峰,肺微血管通透指數(shù)以6h最高,炎癥細(xì)胞浸潤(rùn)以6～12h最顯著。LPS在1h已顯著高于對(duì)照組(P0.01),6h達(dá)最高。PMN凋亡率于灌注后1h一過(guò)性升高,3h顯著抑制,6h最低,以后逐漸接近對(duì)照水平。PMN計(jì)數(shù)與凋亡率呈負(fù)相關(guān)(P0.05)。各海水灌注組血清TNF-α、IL-1β、IL-10均顯著高于對(duì)照組(P0.05或P0.01),與LPS呈正相關(guān)(P0.05或P0.01),但與PMN凋亡率無(wú)相關(guān)性(P0.05)。結(jié)論P(yáng)MN凋亡在SWD-ALI炎癥反應(yīng)早期受到顯著抑制,是最主要的炎癥效應(yīng)細(xì)胞。TNF-α、IL-1β、IL-10在SWD-ALI中發(fā)揮重要作用,但對(duì)PMN凋亡抑制無(wú)顯著影響。
[Abstract]:Objective to investigate the evolution of (PMN) apoptosis in peripheral blood neutrophils during seawater drowning acute lung injury (SWD-ALI) and the possible effect of cytokines on it. Methods 36 New Zealand rabbits were randomly divided into control group (as 0 h baseline value) and 1, 3, 6, 12, 24 h group (1, 3, 6, 12, 24 h after seawater perfusion, respectively). The changes of hemogram and blood gas analysis indexes were observed, the lung wet / dry weight ratio (W 鈮，

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