【摘要】：目的:探討模擬失重30 d后再超重對(duì)猴牙齦組織趨化因子CCL20及其受體CCR6表達(dá)的影響。方法:23只雄性獼猴隨機(jī)分為4組,即對(duì)照組(A)、失重組(B)、超重組(C)、模擬失重30 d后再超重組(D)。其中,D組根據(jù)載荷量大小又分為4個(gè)亞組,即+11Gx/270 s組(D1)、+13Gx/230 s組(D2)、+15 Gx/200 s組(D3)和+13 Gx/230 s恢復(fù)9 d組(D4)。采用組織學(xué)方法觀察牙齦的形態(tài)學(xué)變化,通過免疫組織化學(xué)、Q-PCR方法檢測(cè)牙齦組織中CCL20、CCR6的表達(dá)情況。采用SPSS17.0軟件包對(duì)數(shù)據(jù)進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果:各實(shí)驗(yàn)組中牙齦結(jié)構(gòu)均未見明顯損傷,但實(shí)驗(yàn)組中的淋巴細(xì)胞及中性粒細(xì)胞浸潤(rùn)較對(duì)照組多。對(duì)照組牙齦組織幾乎不表達(dá)CCL20,但弱表達(dá)CCR6;實(shí)驗(yàn)組CCL20陽(yáng)性表達(dá)主要位于牙齦上皮組織的基底層,CCR6陽(yáng)性表達(dá)主要位于牙齦上皮棘層和基底層。除失重組的CCL20外,各實(shí)驗(yàn)組牙齦組織中的CCL20和CCR6在蛋白水平、mRNA水平上的表達(dá)均顯著高于對(duì)照組(P0.05)。結(jié)論:模擬失重30 d后再超重環(huán)境中,猴牙齦組織結(jié)構(gòu)未見明顯改變,但牙齦組織趨化因子CCL20及其受體CCR6表達(dá)明顯增強(qiáng)。
[Abstract]:Aim: to investigate the effect of overweight on the expression of chemokine CCL20 and its receptor CCR6 in gum tissue of monkeys after 30 days of simulated weightlessness. Methods: 23 male rhesus monkeys were randomly divided into 4 groups: control group (A), lost recombinant (B), superrecombinant (C), simulated weightlessness for 30 days, and then superrecombinant (D). Among them, group D was divided into four subgroups according to the load size, namely, 11Gx/270 s group (D1), 13Gx/230 s group (D2), 15 Gx/200 s group (D 3) and 13Gx/230 s recovery 9 d group (D4). The morphological changes of gums were observed by histology, and the expression of CCL20,CCR6 in gum tissues was detected by immunohistochemistry and Q-PCR. SPSS17.0 software package was used to analyze the data statistically. Results: there was no obvious damage to gum structure in each experimental group, but the infiltration of lymphocytes and neutrophils in the experimental group was more than that in the control group. In the control group, there was almost no expression of CCL20, in gum tissue, but the positive expression of CCL20 in the experimental group with weak expression of CCR6; was mainly located in the basal layer of the gum epithelial tissue, and the positive expression of CCR6 was mainly located in the spinous layer and basal layer of the gum epithelial tissue. Except for the lost CCL20, the expression of CCL20 and CCR6 in the gum tissue of each experimental group was significantly higher than that of the control group at the protein level and mRNA level (P 0.05). Conclusion: in the overweight environment after 30 days of simulated weightlessness, the gum tissue structure of monkeys did not change significantly, but the expression of chemokine CCL20 and its receptor CCR6 in gum tissue was significantly increased.
【作者單位】： 解放軍醫(yī)學(xué)院;解放軍第306醫(yī)院全軍口腔疾病診治中心;北京特種醫(yī)學(xué)實(shí)驗(yàn)中心;
【基金】：國(guó)家自然科學(xué)基金(30870598) 全軍醫(yī)藥衛(wèi)生科研基金(06MA004)~~
【分類號(hào)】：R856.78

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