不同氣候地區(qū)武警士兵熱休克蛋白71表達(dá)的差異性分析
發(fā)布時(shí)間:2018-12-11 21:02
【摘要】:目的 分析來自北京、鄭州和廣州三個(gè)地區(qū)武警士兵外周血淋巴細(xì)胞熱休克蛋白 71(HSP71)基礎(chǔ)和熱應(yīng)激后表達(dá)水平的差異性。方法 用流式細(xì)胞術(shù)檢測(cè)淋巴細(xì)胞HSP71水平 ,用彗星試驗(yàn)檢測(cè)淋巴細(xì)胞在基礎(chǔ)和應(yīng)激狀態(tài)下DNA的損傷情況。結(jié)果 彗星試驗(yàn)顯示 ,淋巴細(xì)胞在基礎(chǔ)和熱應(yīng)激條件下 (41℃ ,1h)未發(fā)現(xiàn)有明顯的DNA損傷 ,三個(gè)地區(qū)之間士兵淋巴細(xì)胞在基礎(chǔ)和應(yīng)激條件下DNA損傷的差異均無顯著性 (P 0 .0 5 ) ;熱應(yīng)激后 ,三個(gè)地區(qū)士兵淋巴細(xì)胞HSP71均明顯增高 ,差異有顯著性 (P 0 .0 5 ) ,不同地區(qū)比較發(fā)現(xiàn) ,北京士兵HSP71基礎(chǔ)和熱應(yīng)激水平 (平均熒光強(qiáng)度分別為 84 5 .87± 135 .6 0、12 5 4 .4 7± 2 39.0 5 )均比廣州士兵 (平均熒光強(qiáng)度分別為 70 2 .73± 184 .70、86 1.72±2 2 5 .12 )明顯升高 ,且差異有顯著性 (P 0 .0 5 )。結(jié)論 不同氣候地區(qū)武警士兵淋巴細(xì)胞HSP71基礎(chǔ)和熱應(yīng)激水平存在差異性 ,提示可以將HSP71基礎(chǔ)和熱應(yīng)激水平作為評(píng)價(jià)不同氣候地區(qū)武警戰(zhàn)士在熱環(huán)境下耐受能力的指標(biāo)
[Abstract]:Objective to analyze the differences of heat shock protein 71 (HSP71) expression levels in peripheral blood lymphocytes of armed police soldiers from Beijing, Zhengzhou and Guangzhou. Methods HSP71 levels in lymphocytes were detected by flow cytometry and DNA damage was detected by comet assay. Results Comet assay showed that there was no obvious DNA damage in lymphocytes under basic and heat stress conditions (41 鈩,
本文編號(hào):2373217
[Abstract]:Objective to analyze the differences of heat shock protein 71 (HSP71) expression levels in peripheral blood lymphocytes of armed police soldiers from Beijing, Zhengzhou and Guangzhou. Methods HSP71 levels in lymphocytes were detected by flow cytometry and DNA damage was detected by comet assay. Results Comet assay showed that there was no obvious DNA damage in lymphocytes under basic and heat stress conditions (41 鈩,
本文編號(hào):2373217
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