艙室內(nèi)爆炸對大鼠行為及海馬NO、MDA、SOD的影響
發(fā)布時間：2018-09-01 21:17
【摘要】： 裝甲車、坦克、艦船等帶有艙室的兵器在現(xiàn)代戰(zhàn)爭中具有舉足輕重的作用。艙室內(nèi)爆炸對指戰(zhàn)員的生理和心理造成了嚴重的影響,其傷型和嚴重程度都有別于其它爆炸傷。然而,以往研究大多局限于艙室內(nèi)爆炸臟器損傷的特點,對艙室內(nèi)爆炸致傷所引起的學習記憶和行為及其腦內(nèi)氧自由基變化未見報道。因此,探討艙室內(nèi)爆炸的致傷生理和心理機制,對密閉艙室戰(zhàn)創(chuàng)傷的救治,減少戰(zhàn)時衛(wèi)生減員,具有一定的軍事理論價值和現(xiàn)實意義。 本研究通過按人鼠比例等比縮小模擬某型裝甲運兵車艙室的密閉環(huán)境,采用紙質(zhì)點爆源引爆,構(gòu)建艙室內(nèi)爆炸沖擊傷應激大鼠模型,致傷后,觀察大鼠不同時相點的學習記憶、行為變化,檢測大鼠海馬組織一氧化氮、丙二醛和超氧化物歧化酶含量動態(tài)變化。研究共分兩部分進行:①探討艙室內(nèi)爆炸大鼠學習記憶及海馬氧自由基的變化特點,建立艙室內(nèi)爆炸沖擊傷應激大鼠模型;②在成功建立動物模型的基礎(chǔ)上,觀察不同時相點艙室內(nèi)爆炸和開闊地爆炸大鼠學習記憶、行為和海馬內(nèi)氧自由基變化的異同。研究結(jié)果如下: 1.初步建立了艙室內(nèi)爆炸沖擊傷應激大鼠模型,經(jīng)曠場實驗、Morris水迷宮實驗等證實,該模型能較好的模擬艙室內(nèi)爆炸應激反應的行為學變化特點,且致傷條件容易控制,復制簡單,模型較穩(wěn)定,為開展相關(guān)研究奠定了基礎(chǔ)。 2.不同當量炸藥艙室內(nèi)爆炸后,600mg組、400mg組大鼠的Morris水迷宮逃避潛伏期、曠場水平運動和垂直運動得分較致傷前均顯著增加(p0.01);200mg組大鼠水迷宮逃避潛伏期的變化與致傷前比較呈現(xiàn)出先低后高的現(xiàn)象,但無顯著差異(p0.05),曠場水平運動和垂直運動得分較致傷前無顯著差異(p0.05)。 3.不同當量炸藥艙室內(nèi)爆炸后,200mg組大鼠海馬內(nèi)SOD含量顯著增高(p0.01),而NO、MDA含量變化并不顯著(p0.05);600mg、400mg組大鼠海馬內(nèi)NO、MDA、SOD含量顯著升高(p0.01),本研究結(jié)果艙室內(nèi)爆炸大鼠海馬內(nèi)SOD的升高可能因應激狀態(tài)下海馬內(nèi)NO、MDA等氧自由基產(chǎn)生過多而誘SOD的升高。 4.艙室內(nèi)和開闊地相同當量炸藥爆炸后,密閉組和開放組大鼠在曠場行為實驗中水平運動和垂直運動的得分、迷宮潛伏期及穿梭箱平均反應時間均顯著高于對照組(p0.01)。致傷后,艙內(nèi)外大鼠學習記憶能力、行為改變的差異,主要集中在致傷后12小時至3天之間,在致傷后24小時差異最顯著。曠場行為實驗,密閉組的水平得分和垂直得分炸后前3天顯著高于開放組;Morris水迷宮逃避潛伏期時長,炸后12小時至3天密閉組較開放組顯著增加(p0.01);穿梭箱實驗,密閉組平均反應時在炸后24小時到3天較開放組顯著延長(p0.01)。 5.艙室內(nèi)和開闊地相同當量炸藥爆炸后,密閉組與開放組大鼠海馬氧自由基動態(tài)變化趨勢相同,NO、MDA較對照組均在致傷后6小時始顯著增加(p0.05),24小時達高峰(p0.01);SOD在致傷后1小時較對照組出現(xiàn)顯著差異(p0.01),6小時達高峰后快速降低。致傷后,密閉組與開放組之間氧自由基含量差異顯著:傷后12小時、3天密閉組NO含量顯著高于開放組(p0.01),傷后12小時密閉組SOD顯著高于開放組(p0.01),MDA變化也不同,但無統(tǒng)計學意義(p0.05)。 總之,艙室內(nèi)爆炸可以影響大鼠的學習記憶能力及海馬氧自由基含量,不同當量炸藥對大鼠學習記憶能力及其海馬氧自由基含量影響不同。200mg的炸藥可以改善大鼠的學習記憶能力,減輕氧自由基堆積對機體的損害,400mg、600mg炸藥的爆炸結(jié)果則相反。艙室內(nèi)和開闊地相同當量炸藥爆炸后不同時相點,大鼠的學習記憶能力及海馬氧自由基較對照組均發(fā)生明顯的變化;相比艙室內(nèi)和開闊地爆炸對大鼠學習記憶、行為改變以及氧自由基變化的程度以艙室內(nèi)為重,主要表現(xiàn)在致傷后12小時至3天之間,尤以傷后24小時這一時段最為嚴重。這一結(jié)論對艙室內(nèi)戰(zhàn)傷的救治以及救治介入時機的選擇具有重要意義。
[Abstract]:Armored vehicles, tanks, ships and other weapons with compartments play an important role in modern warfare. Indoor explosion has a serious impact on the physiology and psychology of the fighters. Its injury type and severity are different from other explosive injuries. The changes of learning memory and behavior and oxygen free radicals in brain caused by explosion injury have not been reported. Therefore, exploring the physiological and psychological mechanism of explosion injury in the cabin has certain military theoretical value and practical significance for the treatment of war wounds in airtight cabin and the reduction of health personnel in wartime.
In this study, a rat model of explosive blast injury was constructed by simulating the closed-loop environment of an armored personnel carrier cabin in the same ratio of human to rat. After injury, the learning and memory of rats were observed at different time points, and the behavior changes were detected. Nitric oxide, malondialdehyde and superoxide dismutase (SOD) in hippocampus were detected. The study was divided into two parts: (1) To investigate the changes of learning and memory and hippocampal oxygen free radicals in rats exposed to cabin explosion, and to establish a stress rat model of cabin explosion injury; (2) To observe the learning and memory of rats exposed to cabin explosion and open explosion at different time points on the basis of successful establishment of animal models. The similarities and differences between behavior and oxygen free radicals in hippocampus were studied.
1. The rat model of explosive blast injury in cabin was established. The open field experiment and Morris water maze experiment showed that the model could simulate the behavioral changes of explosive stress in cabin well, and the injury conditions were easy to control, the replication was simple and the model was stable, which laid a foundation for the related research.
2. After explosion in different equivalent explosive chamber, the escape latency, open field horizontal and vertical movement scores of Morris water maze in 600 mg group and 400 mg group were significantly higher than those before injury (p0.01); the escape latency of water maze in 200 mg group was lower than that before injury, but there was no significant difference (p0.05). The score of horizontal movement and vertical movement was not significantly different from that before injury (P0.05).
3. After explosion in different equivalent explosive chamber, SOD content in hippocampus of rats in 200 mg group increased significantly (p0.01), but NO and MDA content did not change significantly (p0.05); NO, MDA and SOD content in hippocampus of rats in 600 mg and 400 mg group increased significantly (p0.01). The results showed that the increase of SOD in hippocampus of rats exposed to explosive chamber might be due to oxygen such as NO and MDA in hippocampus under stress. The excessive production of free radicals induces the increase of SOD.
4. The scores of horizontal and vertical movement, the latency of maze and the average reaction time of shuttle box in the closed and open groups were significantly higher than those in the control group (p0.01). After the explosion, the differences of learning and memory abilities and behavior changes were mainly concentrated in the rats inside and outside the cabin. In open-field behavior test, the horizontal and vertical scores in the closed group were significantly higher than those in the open group at the first three days after explosion; Morris water maze escape latency was longer than that in the open group at 12 hours to 3 days after explosion; shuttle box test showed that the average reaction time in the closed group was significantly longer than that in the open group at 12 hours to 3 days after explosion (p0.01). The duration from 24 hours to 3 days after the blast was significantly longer than that in the open group (P0.01).
5. After the explosion of the same equivalent explosive in the cabin and open space, the dynamic changes of oxygen free radicals in the hippocampus of rats in the closed group and the open group were the same. NO and MDA increased significantly at 6 hours after injury (p0.05) and reached the peak at 24 hours (p0.01); SOD decreased significantly at 1 hour after injury (p0.01) and at 6 hours after the peak. After injury, the content of oxygen free radicals was significantly different between the closed group and the open group. The content of NO in the closed group was significantly higher than that in the open group at 12 hours and 3 days after injury (p0.01). SOD in the closed group was significantly higher than that in the open group at 12 hours after injury (p0.01). The change of MDA was not statistically significant (p0.05).
In a word, compartment explosion can affect the learning and memory ability of rats and the content of oxygen free radicals in hippocampus. Different equivalent explosives have different effects on the learning and memory ability of rats and the content of oxygen free radicals in hippocampus. Compared with the control group, the learning and memory abilities and oxygen free radicals in the hippocampus of rats were significantly changed at different time points after the explosion of the same equivalent explosive in the cabin and open space. From 12 hours to 3 days after injury, especially 24 hours after injury, this conclusion is of great significance to the treatment of war wounds in cabins and the timing of intervention.
【學位授予單位】：第三軍醫(yī)大學
【學位級別】：碩士
【學位授予年份】：2008
【分類號】：R82
[Abstract]:Armored vehicles, tanks, ships and other weapons with compartments play an important role in modern warfare. Indoor explosion has a serious impact on the physiology and psychology of the fighters. Its injury type and severity are different from other explosive injuries. The changes of learning memory and behavior and oxygen free radicals in brain caused by explosion injury have not been reported. Therefore, exploring the physiological and psychological mechanism of explosion injury in the cabin has certain military theoretical value and practical significance for the treatment of war wounds in airtight cabin and the reduction of health personnel in wartime.
In this study, a rat model of explosive blast injury was constructed by simulating the closed-loop environment of an armored personnel carrier cabin in the same ratio of human to rat. After injury, the learning and memory of rats were observed at different time points, and the behavior changes were detected. Nitric oxide, malondialdehyde and superoxide dismutase (SOD) in hippocampus were detected. The study was divided into two parts: (1) To investigate the changes of learning and memory and hippocampal oxygen free radicals in rats exposed to cabin explosion, and to establish a stress rat model of cabin explosion injury; (2) To observe the learning and memory of rats exposed to cabin explosion and open explosion at different time points on the basis of successful establishment of animal models. The similarities and differences between behavior and oxygen free radicals in hippocampus were studied.
1. The rat model of explosive blast injury in cabin was established. The open field experiment and Morris water maze experiment showed that the model could simulate the behavioral changes of explosive stress in cabin well, and the injury conditions were easy to control, the replication was simple and the model was stable, which laid a foundation for the related research.
2. After explosion in different equivalent explosive chamber, the escape latency, open field horizontal and vertical movement scores of Morris water maze in 600 mg group and 400 mg group were significantly higher than those before injury (p0.01); the escape latency of water maze in 200 mg group was lower than that before injury, but there was no significant difference (p0.05). The score of horizontal movement and vertical movement was not significantly different from that before injury (P0.05).
3. After explosion in different equivalent explosive chamber, SOD content in hippocampus of rats in 200 mg group increased significantly (p0.01), but NO and MDA content did not change significantly (p0.05); NO, MDA and SOD content in hippocampus of rats in 600 mg and 400 mg group increased significantly (p0.01). The results showed that the increase of SOD in hippocampus of rats exposed to explosive chamber might be due to oxygen such as NO and MDA in hippocampus under stress. The excessive production of free radicals induces the increase of SOD.
4. The scores of horizontal and vertical movement, the latency of maze and the average reaction time of shuttle box in the closed and open groups were significantly higher than those in the control group (p0.01). After the explosion, the differences of learning and memory abilities and behavior changes were mainly concentrated in the rats inside and outside the cabin. In open-field behavior test, the horizontal and vertical scores in the closed group were significantly higher than those in the open group at the first three days after explosion; Morris water maze escape latency was longer than that in the open group at 12 hours to 3 days after explosion; shuttle box test showed that the average reaction time in the closed group was significantly longer than that in the open group at 12 hours to 3 days after explosion (p0.01). The duration from 24 hours to 3 days after the blast was significantly longer than that in the open group (P0.01).
5. After the explosion of the same equivalent explosive in the cabin and open space, the dynamic changes of oxygen free radicals in the hippocampus of rats in the closed group and the open group were the same. NO and MDA increased significantly at 6 hours after injury (p0.05) and reached the peak at 24 hours (p0.01); SOD decreased significantly at 1 hour after injury (p0.01) and at 6 hours after the peak. After injury, the content of oxygen free radicals was significantly different between the closed group and the open group. The content of NO in the closed group was significantly higher than that in the open group at 12 hours and 3 days after injury (p0.01). SOD in the closed group was significantly higher than that in the open group at 12 hours after injury (p0.01). The change of MDA was not statistically significant (p0.05).
In a word, compartment explosion can affect the learning and memory ability of rats and the content of oxygen free radicals in hippocampus. Different equivalent explosives have different effects on the learning and memory ability of rats and the content of oxygen free radicals in hippocampus. Compared with the control group, the learning and memory abilities and oxygen free radicals in the hippocampus of rats were significantly changed at different time points after the explosion of the same equivalent explosive in the cabin and open space. From 12 hours to 3 days after injury, especially 24 hours after injury, this conclusion is of great significance to the treatment of war wounds in cabins and the timing of intervention.
【學位授予單位】：第三軍醫(yī)大學
【學位級別】：碩士
【學位授予年份】：2008
【分類號】：R82
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