本文選題：肝炎 + 戊型��； 參考：《中華傳染病雜志》2006年02期

【摘要】：目的了解廣州某部新兵連戊型病毒性肝炎(戊型肝炎)暴發(fā)流行的分子病毒學(xué)特征,并與當?shù)厣l(fā)毒株比較,以查找病原可能來源。方法用逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(RT-PCR)法,對抗HEV-IgM陽性的34例暴發(fā)性戊型肝炎及46例散發(fā)性戊型肝炎患者的血清和糞便標本進行HEVRNA檢測,并對HEVRNA陽性標本的基因開放讀碼框(ORF)2部分片段進行克隆測序分析。結(jié)果34例暴發(fā)流行病例標本中檢測出12株病毒,46例散發(fā)病例標本中檢到2株。經(jīng)克隆測序分析,各暴發(fā)毒株的核苷酸同源性為95.3%~100%;氨基酸同源性為94.0%~100%。且暴發(fā)毒株和散發(fā)毒株的核苷酸及氨基酸的同源性也較高,分別為95.3%~99.3%和94.0%~100%;暴發(fā)毒株和散發(fā)毒株與各型中的標準株相比,與Jap1株同源性最高,其核苷酸同源性為92.0%~95.3%,氨基酸同源性為96.0%~100.0%。進化樹分析提示本次戊型肝炎暴發(fā)流行病毒株與戊型肝炎病毒基因Ⅳ型距離最近。結(jié)論本次戊型肝炎暴發(fā)流行的病原可能來于廣州本地;廣州地區(qū)戊型肝炎流行毒株屬戊型肝炎基因型Ⅳ型。
[Abstract]:Objective to investigate the molecular virological characteristics of the outbreak of hepatitis E (hepatitis E) in recruits in Guangzhou, and to find out the possible source of the pathogen. Methods the serum and fecal samples of 34 fulminant hepatitis E patients and 46 sporadic hepatitis E patients with HEV-IgM positive were detected by reverse transcription-polymerase chain reaction (RT-PCR). The open reading frame (ORF) 2 fragments of HEV RNA positive samples were cloned and sequenced. Results 12 strains of virus were detected in 34 outbreak cases and 2 of 46 sporadic cases were detected. The nucleotide homology of each outbreak strain was 95.3% and the amino acid homology was 94.0%. The nucleotide and amino acid homology of outbreak strain and sporadic strain were 95.3% and 94.0%, respectively. The nucleotide homology and amino acid homology were 92.0 and 95.3, respectively. The phylogenetic tree analysis indicated that the distance between the outbreak of hepatitis E virus strain and the hepatitis E virus gene type 鈪，

本文編號：2096586