發(fā)布時間：2018-03-21 00:44

  本文選題：硝化菌　切入點：PCR　出處：《解放軍預(yù)防醫(yī)學(xué)雜志》2002年03期 　論文類型：期刊論文

【摘要】：目的 :篩選、分離能夠用于治理氨氮污染和軍事坑道生活污物、污水的硝化菌。方法 :通過富集培養(yǎng)從土壤中篩選的硝化菌 ,在進(jìn)行形態(tài)學(xué)檢驗和硝化速率測定后 ,設(shè)計引物擴(kuò)增硝化菌的特征性基因NorB ,隨機(jī)選擇一個PCR產(chǎn)物進(jìn)行測序 ,并與Genbank上的序列比較同源性。結(jié)果 :篩選到 7株能夠降解亞硝酸鹽的細(xì)菌 ,經(jīng)革蘭氏染色后發(fā)現(xiàn)全部為陰性 ,其菌落形態(tài)以小、圓、淡黃色為主 ,細(xì)菌形態(tài)多為短桿狀 ;各菌株的硝化速率在 4 .35～ 4 .80 (mg·L 1·d 1)之間。用PCR均能擴(kuò)增出預(yù)期大小片段。對其中的某一個PCR產(chǎn)物直接測序 ,與Genbank中的序列比較 ,發(fā)現(xiàn)該基因與硝化菌N .hamburgensis的Norb基因同源性高達(dá) 99%。結(jié)論 :可以初步斷定 ,我們所篩選到的菌為硝化菌
[Abstract]:Objective: to screen and isolate nitrifying bacteria which can be used to control ammonia nitrogen pollution, military tunnel sewage and sewage. Methods: after morphological examination and nitrification rate determination, the nitrifying bacteria selected from soil were enriched and cultured. Primers were designed to amplify the characteristic gene NorB of nitrifying bacteria, and a PCR product was randomly selected for sequencing. Results: seven strains of bacteria capable of degrading nitrite were screened. It was found that all of them were negative by Gram staining. The colony morphology was small, round and light yellow, and most of the bacteria were short rod shape. The nitrification rate of each strain ranged from 4.35 to 4.80 mg 路L 1 路d 1. The expected size fragments could be amplified by PCR. One of the PCR products was sequenced directly and compared with the sequence in Genbank. The homology of this gene with the Norb gene of nitrifying bacteria N. hamburgensis is as high as 99%. Conclusion: it can be preliminarily concluded that the bacteria we screened are nitrifying bacteria.
【作者單位】： 軍事醫(yī)學(xué)科學(xué)院衛(wèi)生學(xué)環(huán)境醫(yī)學(xué)研究所 軍事醫(yī)學(xué)科學(xué)院衛(wèi)生學(xué)環(huán)境醫(yī)學(xué)研究所 軍事醫(yī)學(xué)科學(xué)院衛(wèi)生學(xué)環(huán)境醫(yī)學(xué)研究所 軍事醫(yī)學(xué)科學(xué)院衛(wèi)生學(xué)環(huán)境醫(yī)學(xué)研究所 軍事醫(yī)學(xué)科學(xué)院衛(wèi)生學(xué)環(huán)境醫(yī)學(xué)研究所 軍事醫(yī)學(xué)科學(xué)院衛(wèi)生學(xué)環(huán)境醫(yī)學(xué)研究所 軍事醫(yī)學(xué)科學(xué)院衛(wèi)生學(xué)環(huán)境醫(yī)學(xué)研究所
【分類號】：R82

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 金志剛,屈計寧,何群彪;硝化細(xì)菌富集技術(shù)分析及方法研究[J];上海環(huán)境科學(xué);1998年08期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 李谷,黃正,范瑋,劉紅艷;硝化細(xì)菌富集方法的研究[J];淡水漁業(yè);2000年09期

2 陳際達(dá),陳志勝,張光輝,唐昌敏;含氮廢水亞硝化型硝化的研究[J];重慶大學(xué)學(xué)報(自然科學(xué)版);2000年03期

3 陳際達(dá),曲中堂,鄧s，

本文編號：1641519